Hycult Biotech/Properdin，小鼠，单克隆抗体H4/HM1139/100µg
商品编号：
HM1139
品牌：
hycultbiotech
市场价：
¥8736.00
美元价：
5241.60
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:Properdin,Mouse,mAbH4
MonoclonalantibodyH4recognizesmouseproperdin.Thecomplementsystemisthefirstlineofdefenseagainstpathogensandfacilitateseliminationofapoptoticanddamagedcells.Positiveregulatorplasmaproteinproperdiniscriticalforthealternativepathwayofcomplement.Itisasingle-chainglycoprotein(ca53kDa)consistingofsixTSRsequences.Intheblooditexistsasamixtureofpreferablyhead-to-tailtrimers,butalsodimersandtetramers.Theproteinisproducedbyleukocytes,likeactivatedneutrophilsmonocytesandT-lymphocytes,butalsobyeg.stressedendothelialcells.
ProperdincanbothinitiateandpositivelyregulatethealternativepathwayactivitytogetherwithC3andfactorsB,D,IandH.ItbindstoC3bwhereItstABIlizesthelabileC3bBbconvertasewhichisdepositedonimmunecomplexesorforeignsurfaces.TherebyenhancingtheAPbystimulationofamplificationofC3bBb-convertaseformationincompetitionwithcatabolismofC3bbyfactorI,whichusesfactorHasacofactor.ThelocalamplificationprocessleadstothecreationofthealternativepathwayC5convertase,C3bBb3b,andinitiatestheterminalpathwayofcomplementactivation.Thealternativepathwaymayaccountforca80%oftheterminalpathwayactivity.ProperdinhasalsobeenshowntodirectlylimitfactorHactivity.
Recentstudiesshowthatproperdinisalsoapattern-recognitionreceptor(PRR)abletobinddirectlytomicrobialsurfaces,apoptoticandnecroticcells(dangerousnonselfandalteredself).Inappropriateactivationordysregulationofthealternativepathwayisacriticalfactorindevelopmentofseveralautoimmuneconditions.Targetsopsonizedwithproperdinarelabeledforclearancebyscavengercells,evenwithoutcomplement.Thismakesitapotentialtherapeutictargetindiseases.Recentstudieshasshownrenewedinterestintheevaluatingroleofproperdinindiseasepathogenesis,likeAsthma,arthritis,septicshock,AMDandC3glomerulopathy.
ProperdincanbothinitiateandpositivelyregulatethealternativepathwayactivitytogetherwithC3andfactorsB,D,IandH.ItbindstoC3bwhereItstABIlizesthelabileC3bBbconvertasewhichisdepositedonimmunecomplexesorforeignsurfaces.TherebyenhancingtheAPbystimulationofamplificationofC3bBb-convertaseformationincompetitionwithcatabolismofC3bbyfactorI,whichusesfactorHasacofactor.ThelocalamplificationprocessleadstothecreationofthealternativepathwayC5convertase,C3bBb3b,andinitiatestheterminalpathwayofcomplementactivation.Thealternativepathwaymayaccountforca80%oftheterminalpathwayactivity.ProperdinhasalsobeenshowntodirectlylimitfactorHactivity.
Recentstudiesshowthatproperdinisalsoapattern-recognitionreceptor(PRR)abletobinddirectlytomicrobialsurfaces,apoptoticandnecroticcells(dangerousnonselfandalteredself).Inappropriateactivationordysregulationofthealternativepathwayisacriticalfactorindevelopmentofseveralautoimmuneconditions.Targetsopsonizedwithproperdinarelabeledforclearancebyscavengercells,evenwithoutcomplement.Thismakesitapotentialtherapeutictargetindiseases.Recentstudieshasshownrenewedinterestintheevaluatingroleofproperdinindiseasepathogenesis,likeAsthma,arthritis,septicshock,AMDandC3glomerulopathy.
Specifications:
| Catalognumber | HM1139 |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 100µg |
| Formulation | 1ml(100μg/ml)0.2μmproteinGpurifiedantibodysolutioninPBS. |
| Application | Functionalstudies,Immunoassays,Immunofluorescence,Westernblot |
| ApplicationNotes | W:AreducedsampletreatmentandSDS-Pagewasused.Thebandsizefornativeproperdinis~52kDaandtheantibodyrecognizesrecombinantTSR5/6antigenwhichhasabandsizeof~18kDa(Ref.2). |
| Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
| Immunogen | Recombinanthis-taggedmTSR5/6isolatedfromE.coli(refolded) |
| Isotype | ArmenianhamsterIgG |
| Species | Mouse |
| Alias | ComplementfactorP Genename:Cfp,Pfc |
| References | 1.ZhouHetal;Afibrinogen-specificantibodyinducesaBDominalaorticaneurysminmicethroughcomplementlectinpathwayactivation.Proc.Natl.Acad.Sci.USA2013,110:E4335 2.Bertram,Petal;Anti-MouseProperdinTSR5/6MonoclonalAntibodiesBlockComplementAlternativePathway-dependentPathogenesis.MonoclonAntibImmunodiagnImmunother2015,34:1. |
| Storageandstability | Productshouldbestoredat4°C.Underrecommendedstorageconditions,productisstableforatleastoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
| Disease | Autoimmunity,Infectiousdiseases,Nephrology |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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