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Hycult Biotech/C4d, Human, mAb 12D11, immuno assay antibody/HM2229-IA/0.5 mg
Hycult Biotech/C4d, Human, mAb 12D11, immuno assay antibody/HM2229-IA/0.5 mg
商品编号：
HM2229-IA
品牌：
hycultbiotech
市场价：
¥21740.00
美元价：
13044.00
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:C4d,Human,mAb12D11,immunoassayantibody
Themonoclonalantibody12D11(alsoknownascloneM4d3)recognizesanepitopeonthealpha2chainofhumanC4d.ComplementfactorC4(MW41kDa),formerlyknownasGgprotein,consistsofanalpha-,beta-andgamma-chain.Theclassicalpathwayofcomplementandthemannosebindinglectin(MBL)activationpathwayconvergeatC4.ActivatedC1,MASP-1andMASP-2cleaveC4resultingintheformationofC4aandC4b.ThelattercanbecleavedbyfactorIresultinginC4candC4d,inwhichstepallfunctionalsitesarelost.TheC4dactivationfragmentofC4isanexcellentMarkerforclassicalcomplementpathwayandMBLpathwayactivation.ThethioesterformedbetweenthesidechainsofCys1010andGln1013withintheC4dregionoftheα-chainmediatescovalentattachmenttothetargetsurfacebearingactivatedformsofC1sorMASP.
FurThermore,C4dishighlyhomologoustoC3dwithover35%sharedaminoacidsequence.Inanumberofdiseasessuchasrheumatoidarthritis(RA),hereditaryangioedema(HAE),systemiclupuserythematosus(SLE)andchronicurticariawithhypercomplementemialevelsofC4daresignificantlyelevatedinserumorplasma.C4dlevelsmayalsobeelevatedinplasmafrompatientswithavarietyofhumoralautoimmunediseasesinwhichcomplementactivationisknowntooccur.DepositionofC4dinperitubularcapillarieshasbeenshowntobeasensitivemarkerforantibody-mediated(humoral)rejectioninrenaltransplantbiopsies.Themonoclonalantibody12D11recognizesC4,C4bandC4d.Antibody12D11crossreactswithpig,bovine,baboonandrhesusmonkey.
FurThermore,C4dishighlyhomologoustoC3dwithover35%sharedaminoacidsequence.Inanumberofdiseasessuchasrheumatoidarthritis(RA),hereditaryangioedema(HAE),systemiclupuserythematosus(SLE)andchronicurticariawithhypercomplementemialevelsofC4daresignificantlyelevatedinserumorplasma.C4dlevelsmayalsobeelevatedinplasmafrompatientswithavarietyofhumoralautoimmunediseasesinwhichcomplementactivationisknowntooccur.DepositionofC4dinperitubularcapillarieshasbeenshowntobeasensitivemarkerforantibody-mediated(humoral)rejectioninrenaltransplantbiopsies.Themonoclonalantibody12D11recognizesC4,C4bandC4d.Antibody12D11crossreactswithpig,bovine,baboonandrhesusmonkey.
Specifications:
| Catalognumber | HM2229-IA |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 0.5mg |
| Formulation | 0.5mgof0.2μmfilteredproteinGpurifiedantibodysolutioninPBS(exactconcentrationisindicatedonthelabel). |
| Application | Flowcytometry,Frozensections,Immunoassays,Westernblot |
| ApplicationNotes | F:PermeABIlizationwasdoneincoldacetonewith0.5%hydrogenperoxidasefor10min,afterdryingandwashing,antibodieswereincubatedfor30minutes. FC:Pneumococciwereincubatedwith25μlofa1:10diluted12D11,incubatedfor30minutesandwashed(2x),followedby30minutesincubationoniceofa1:100dilutedgoat-anti-mouseIgGPEoniceinthedarkandwashed(2x)(Ref.5). |
| Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
| Isotype | MouseIgG1 |
| Species | Human |
| Crossreactivity | Baboon-Yes,Bovine-Yes,Rhesusmonkey-Yes,Swine-Yes |
| References | 1.Zwirner,Jetal;Classicalpathwayofcomplementactivationinnormalanddiseasedhumanglomeruli.KidneyInt1989,36:1069. 2.Zwirner,Jetal;Classicalpathwayofcomplementactivationinmammaliankidneys.Immunology1993,80:162. 3.Feucht,Hetal;CapillarydepositionofC4dcomplementfragmentandearlyrenalgraftloss.KidneyInt1993,43:1333. 4.Rensen,Setal;ActivationoftheComplementSysteminHumanNonalcoholicFattyLiverDisease.Hepatology2009,50:1809 5.Meijvis,Setal;Strategiestoimproveclinicalmanagementofcommunity-acquiredpneumonia.Thesis2012. |
| Storageandstability | Productshouldbestoredat4°C.Underrecommendedstorageconditions,productisstableforatleastoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
| Disease | Autoimmunity,Infectiousdiseases,Nephrology |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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