Hycult Biotech/C3aR，小鼠，单克隆抗体14D4/HM1123/100µg
商品编号：
HM1123
品牌：
hycultbiotech
市场价：
¥6432.00
美元价：
3859.20
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:C3aR,Mouse,mAb14D4
Themonoclonalantibody14D4reactswithmouseC3aR,whichisamemberoftherhodopsinsuperfamilyof7-transmembraneGprotein-coupledreceptors,withamolecularweightofapproximately54kDa.ExpressionofC3aRhasbeendemonstratedonawidevarietyofimmunecells,includingmonocytes,macrophages,dendriticcells,neutrophils,basophils,eosinophils,mastcells,TlymphocytesandBlymphocytes.Inaddition,C3aRisfoundoncellsofthecentralnervoussystem,lungsandkidney.InthecourseofcomplementactivationC3aRfunctionsasthecellsurfacereceptorforC3a,whichistheC-terminal77aminoacidcleavageproductofC3.C3a,possessesbothanaphylatoxicandimmunoregulatoryproperties,suchassmoothmusclecontraction,histaminereleasefrommastcells,andenhancedvascularpermeABIlity.Inaddition,C3ainducesrespiratoryburstinneutrophilsandhaschemotacticpropertiesforeosinophilsandmastcells.Moreover,C3acausesreleaseofkeycytokinesfrommultiplecelltypes,includingIL-1β,TNF-α,IL-6andIL-8.AroleforC3aRhasbeenimplicatedinseveralmurinediseasemodels.ItwasshownthatC3aRinhibitionreducesneurodegenerationinexperimentallupus,whereasinamurinemodelofallergicairwaydisease,deletionoftheC3areceptorprotectsagainstthechangesinlungphysiologyseenafterallergenchallenge.Finally,itwasshownthatdeletionofC3aRisprotectiveinmyelinoligodendrocyteglycoprotein-inducedexperimentalautoimmuneencephalomyelitis.
Specifications:
| Catalognumber | HM1123 |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 100µg |
| Formulation | 1ml(100µg/ml)0.2µmfilteredantibodysolutioninPBS,containing0.1%bovineserumalbuminand0.02%sodiumazide. |
| Application | Flowcytometry,Frozensections |
| ApplicationNotes | FC:Antibody14D4stainstheextracellulardomainofC3aRwhenRBL-2H3transfectantsandhumanmonocytesareused.Primaryantibodyconcentrationswere5µg/ml.(Ref.1)IHC-F:Tissuesectionswerefixedinacetone.ToblockunspecificbindingofAbs,sectionswereincubatedwithPBSandheataggregatedhumanIgGat300µg/ml(Ref.1). |
| Use | Forimmunohistochemistryandflowcytometry,dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
| Immunogen | RBL-2H3transfectantsexpressingmouseC3aR |
| Isotype | RatIgG2a |
| Species | Mouse |
| References | KiafardZ,etal;Useofmonoclonalantibodiestoassessexpressionofanaphylatoxinreceptorsintubularepithelialcellsofhuman,murineandratkidneys.ImmunoBIOLOGy2007,212:129. |
| Storageandstability | Productshouldbestoredat4°C.Underrecommendedstorageconditions,productisstableforatleastoneyear.Theexactexpirydateisindicatedonthelabel. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
| Disease | Infectiousdiseases,Nephrology |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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