Hycult Biotech/MARCO, Human, mAb PLK-1/HM2208/100 µg
商品编号：
HM2208
品牌：
hycultbiotech
市场价：
¥8660.00
美元价：
5196.00
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description: MARCO, Human, mAb PLK-1
The monoclonal antibody PLK-1 recognizes the macrophage receptor with collagenous structure (MARCO). The scavenger receptors (SRs) expressed by macrophages are thought to play an important role in the immune response against bacteria by mediating ligand binding and phagocytosis. SRs can be divided into three different classes based upon their structural properties, which are termed SR-A, SR-B and SR-C. SRs-A are homotrimeric glycoproteins composed of 77 kDa monomers subdivided into 3 types.. The molecular structure of MARCO resembles that of SR-A type I, containing a triple-helical collagenous domain and a scavenger receptor cysteine-rich (SRCR) domain at the C terminus. MARCO is only expressed in some subpopulations of macrophages, although its expression can be strongly upregulated during infection or LPS treatment. Furthermore, MARCO is, like sinusoidal endothelial cell markers DC-SIGNR, LYVE-1 and stabilin-2, expressed by sinusoidal endothelial cells in lymph node. MARCO expressed by alveolar macrophages seems to play an important role in response to inhaled particles and airborne pathogens. The monoclonal antibody PLK-1 binds specifically to MARCO, and has been shown to partially block ligand binding.
Specifications:
| Catalog number | HM2208 |
|---|---|
| Product type | Monoclonal antibodies |
| Quantity | 100 µg |
| Formulation | 1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin. |
| Application | Flow cytometry, Frozen sections, Functional studies, Immuno fluorescence, Immuno precipitation, Western blot |
| Application Notes | IHC-F:- Tissue sections can be- fixed in acetone or 2% paraformaldehyde. PLK-1 antibody was used at 5 µg/ml (Ref. 3, 4). FC: Antibody PLK-1 weakly stains alveolar macrophages by recognizing the extracellular domain of MARCO. Transfected COS cells were used as positive control (Ref. 1). FS: Antibody PLK-1 blocks human alveolar macrophages binding to unopsonized particles (Ref.1,3,5). IF: Aveolar macrophages and transfected CHO cells were stained for MARCO using 0.6 µg/ml PLK-1 antibody (Ref. 2, 5) IP: Antibody PLK-1 immunoprecipitates MARCO as 60 and 50 kDa protein from lysates obtained from COS cells transfected with human MARCO (Ref. 3). W: Antibody PLK-1 stained MARCO under non-reducing conditions (Ref. 6). |
| Use | For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For inhibition of biological activity in vitro dilutions have to be made according to the amounts of MARCO to be inactivated. |
| Immunogen | Human alveolar macrophages (recognizes domain V between residues 420 and 431) |
| Isotype | Mouse IgG3 |
| Species | Human |
| Cross reactivity | Bovine - Yes |
| Alias | SCARA2, scavenger receptor class A member 2, macrophage receptor with collagenous structure |
| Positive Control | Human alveolar macrophages |
| References | 1. Palecanda, A et al; Receptors for unopsonized particles: the role of alveolar macrophage scavenger receptors.. Curr Mol Med 2001, 1: 589 2. Bunn, H et al; MARCO expression on pediatric alveolar macrophages. Cytometry 2004, 60B: 54 3. Arredouani, M, et al; MARCO is the major binding receptor for unopsonized particles and bacteria on human alveolar macrophages. J Immunol 2005, 175: 6058 4. Martens, J-H et al; Differential expression of a gene signature for scavenger/lectin receptors by endothelial cells and macrophages in human lymph node sinuses, the primary sites of regional metastasis. J Pathol 2006, 208: 574 5. Hamilton, R et al; MARCO mediates silica uptake and toxicity in alveolar macrophages from C57BL/6 mice. J Biol Chem 2006, 281: 34218 6. Bowdish, D et al; MARCO, TLR2, and CD14 are required for macrophage cytokine responses to mycobacterial trehalose dimycolate and Mycobacterium tuberculosis. PloS Pathog 2009, 5: e1000474 |
| Storage and stability | Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year. |
| Precautions | For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use or derivation of this product. |
| Disease | Infectious diseases |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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