Hycult Biotech/PAI-1, Human, mAb MA-33H1F7/HM2179/100 µg
商品编号：
HM2179
品牌：
hycultbiotech
市场价：
¥6432.00
美元价：
3859.20
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:PAI-1,Human,mAbMA-33H1F7
Plasminogenactivatorinhibitortype-1(PAI-1),amemberoftheserineproteaseinhibitor(serpin)superfamily,isanimportantproteinintheregulationoffibrinolysis.PAI-1isuniqueamongtheserpinsbecauseofitsfunctionalandconformationalflexibility.PAI-1isthemostimportantphysiologicalinhibitorofbothtissue-typeplasminogenactivator(t-PA)andurokinase-typeplasminogenactivator(u-PA).IncreasedPAI-1levelsareassociatedwiththromboticeventsandisanestablishedriskfactorforcardiovasculardiseases.TheactiveconformationPAI-1inhibitsitstargetproteinasesbytheformationofastable,inactivecomplex.AlthoughPAI-1issynthesizedasanactivemolecule,itconvertsspontaneouslytoaninactive,latentformthatcanbepartiallyreactivatedbydenaturingagents.Inaddition,athirdconformationreactingasanon-inhibitorysubstratetowardsvarioustargetproteinaseshasbeenidentified.
TheepitopeofmonoclonalantibodyMA-33H1F7ispredominantlycomposedofthreeresidues(Lys154/Glu130/Arg131),positionedvirtuallylinearlyinthethree-dimensionalstructure.Theepitopeoftheantibodydoesnotcoverthecompletealpha-helixFandturnconnectingalpha-helixFandbeta-strands3A,butisrestrictedtothehingeregionbetweenalpha-helixFandthemainpartofthePAI-1molecule.
ThemonoclonalantibodyMA-33H1F7isa‘switching’antibody,capableofinducinganon-inhibitorysubstrateformofPAI-1.ItwasshowntoinhibitPAI-1inadosedependentmanner.
TheepitopeofmonoclonalantibodyMA-33H1F7ispredominantlycomposedofthreeresidues(Lys154/Glu130/Arg131),positionedvirtuallylinearlyinthethree-dimensionalstructure.Theepitopeoftheantibodydoesnotcoverthecompletealpha-helixFandturnconnectingalpha-helixFandbeta-strands3A,butisrestrictedtothehingeregionbetweenalpha-helixFandthemainpartofthePAI-1molecule.
ThemonoclonalantibodyMA-33H1F7isa‘switching’antibody,capableofinducinganon-inhibitorysubstrateformofPAI-1.ItwasshowntoinhibitPAI-1inadosedependentmanner.
Specifications:
| Catalognumber | HM2179 |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 100µg |
| Formulation | 1ml(100µg/ml)0.2µmfilteredantibodysolutioninPBS,containing0.1%bovineserumalbumin. |
| Application | Functionalstudies,Immunoassays,Westernblot |
| ApplicationNotes | W:Anon-reducedsampletreatmentandSDS-Pagewasused.Thebandsizeis52kDa(Ref.5). FS:AntibodyMA-33H1F7functionsasanantagoNIST.Theantibodywas-incubatedwithactivePAI-1andresidualactivitywasmeasuredbyafunctionalassay(Ref.1). |
| Use | ForWesternblotting,dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.Forfunctionalstudies,dilutionshavetobeoptimizedinuser"sexperimentalsetting. |
| Immunogen | HumanPAI-1/t-PAcomplex |
| Isotype | MouseIgG1 |
| Species | Human |
| Crossreactivity | Mouse-Yes,Rabbit-Yes,Rat-Yes |
| Alias | PAI-1,endothelialplasminogenactivatorinhibitor,serpinE1 |
| References | 1.Debrock,Setal;Neutralizationofplasminogenactivatorinhibitor-1inhibitoryproperties:identificationoftwodifferentmechanisms.BiochimBiophysActa1997,1337:257 2.Berry,Cetal;Antithromboticactivityofamonoclonalantibodyinducingthesubstrateformofplasminogenactivatorinhibitortype1inratmodelsofvenousandarterialthrombosis.BrJPharm1998,125:29 3.Bijnens,Aetal;Importanceofthehingeregionbetweenalpha-helixFandthemainpartofserpins,baseduponidentificationoftheepitopeofplasminogenactivatorinhibitortype1neutralizingantibodies.JBiolChem2000,275:6375 4.Rupin,Aetal;Inactivationofplasminogenactivatorinhibitor-1acceleratesthrombolysisofaplatelet-richthrombusinratmesentericarterioles.ThrombHaemst2001,86:1528 5.Sironi,Letal;Effectofvalsartanonangiotensinll-inducedplasminogenactivatorinhibitor-1biosynthesisinarterialsmoothmusclecells.JAmHeartAss2001,37:961 6.Komissarov,Aetal;RedirectionofthereactionbetweenactivatedproteinCandaserpintothesubstratepathway.ThrombRes.2008,122:397 |
| StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforatleastoneyear.Theexactexpirydateisindicatedonthelabel. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
| Disease | Cardiologyandmetabolism,Tumorimmunology |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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