Hycult Biotech/Elastase, Human, mAb 265-3K1/HM2174/100 µg
商品编号：
HM2174
品牌：
hycultbiotech
市场价：
¥7752.00
美元价：
4651.20
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:Elastase,Human,mAb265-3K1
Themonoclonalantibody265-3K1recognizeshumanleukocyteelastase.Leukocyteelastase,amajorserineproteinaseinman,ispredominantlypresentintheazurophilicgranulesofneutrophilsandmonocytes.Elastasehasabroadrangeofextracellularmatrixsubstratesincludingelastin,proteoglycans,collagenandfibronectin.Theactionofelastaseiscontrolledbyserineproteinaseinhibitors.Elastase,whenreleasedduringinflammation,israpidlyboundbyitstwomaininhibitors,alpha1-PIandalpha2-macroglobulinetoformelastase-inhibitorcomplexes.Inadditionmucosasecretionsmaycontainthelocallysecretedelastaseinhibitorselafin/SKALPandSLPI.Whensecretedatsitesofinflammationelastasecancauseseveretissuedamage.Animportantrolehasbeensuggestedforhumanelastaseinvariousinflammatorydisorders,includingpulmonaryemphysema,sepsis,arthritis,nephritisandcertainskindiseases.ElastaseinducestheproductionofIL-8inhumanbronchialepithelial,aprocesthatoccursinpartthroughTLR4.
Specifications:
| Catalognumber | HM2174 |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 100µg |
| Formulation | 1ml(100µg/ml)0.2µmfilteredproteinGpurifiedantibodysolutioninPBS,containing0.02%sodiumazideand0.1%bovineserumalbumin. |
| Application | Flowcytometry,Immunoassays,Immunofluorescence,Paraffinsections,Westernblot |
| ApplicationNotes | IA:theantibodycanbeusedascaptureantibody.Antibody265-3K1wasuniqueinthatthelimitofdetectionasacaptureantibodyincreasedfrom625nM(18.8ng/ml)forfreeHNEto80nMforHNE–AATcomplex,indicatingthatcovalentcomplexformationbetweenHNEandAATincreasesantibodyaffinityorincreasesexposureofrecognisedepitopeinHNEfor265-3K1(Ref.1). FC:cellswerefixedwithADGfixationbufferAfor15minatroomtemperature.Cellswerewashedandincubatedwith10μgofmonoclonalanti-HNEantibodydilutedinADGpermeABIlisationbufferB(Ref.1). IF:tissueswerefixedinparaformaldehyde(PFA)3.7%,embeddedinparaffinandsectionedat6mm.Thesectionsweredeparaffinizedintolueneandhydratedingradedseriesofethanol(Ref.2). |
| Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
| Isotype | MouseIgG1 |
| Species | Human |
| References | 1.Davies,Petal;Monoclonalanti-neutrophilelastaseantibodycharacterisation:abilitytoblockfunction,detectfreeversusserpin-complexedenzymeandstainintracellulargranules.JImmunol.Meth.2008,336:175 2.Delbosc,Setal;PorphyromonasgingivalisParticipatesinPathogenesisofHumanABDominalAorticAneurysmbyNeutrophilActivation.ProofofConceptinRats.PLosONE2011,6:e18679 3.Novak,Tetal;OralUlcerationinBehçet"sDisease:AnInvestigationofNeutrophilElastaseandItsInhibitors.Thesis2013 |
| Storageandstability | Productshouldbestoredat4°C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
| Disease | Autoimmunity,Pulmonology |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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