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当前位置: 首页 > 产品中心 > monoclonal_antibody > Hycult Biotech/Macrophages mature, Human, mAb 25F9, biotinylated/HM2158BT/50 µg
商品详细Hycult Biotech/Macrophages mature, Human, mAb 25F9, biotinylated/HM2158BT/50 µg
Hycult Biotech/Macrophages mature, Human, mAb 25F9, biotinylated/HM2158BT/50 µg
Hycult Biotech/Macrophages mature, Human, mAb 25F9, biotinylated/HM2158BT/50 µg
商品编号: HM2158BT
品牌: hycultbiotech
市场价: ¥4896.00
美元价: 2937.60
产地: 美国(厂家直采)
公司:
产品分类: 单克隆抗体
公司分类: monoclonal_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Description: Macrophages mature, Human, mAb 25F9, biotinylated

The monoclonal antibody 25F9 recognises a protein of 86 kD on the cell surface and within the cytoplasm of mature macrophages. The antibody is associated with fully differentiated tissue macrophages both in normal and in diseased tissues, independently of the presence or absence of inflammation. The antigen is absent on freshly isolated monocytes and other blood cells. After 6 to 7 days culture human monocytes become positive. Some melanoma and carcinoma cell lines are also positive. Furthermore the monoclonal antibody 25F9 cross reacts with a subpopulation of macrophages of rhesus monkey, pig alveolar macrophages and Kupffer cells. The monoclonal antibody 25F9 is very useful for macrophage phenotyping.

Specifications:

Catalog number HM2158BT
Product type Monoclonal antibodies
Quantity 50 µg
Formulation 0.5 ml (100 µg/ml) 0.2 µm filtered biotinylated antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Conjugate Biotin
Application Flow cytometry, Frozen sections, Paraffin sections, Western blot
Use For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Isotype Mouse IgG1
Species Human
Cross reactivity Rhesus monkey - Yes
References 1. Rosseau, S et al; Phenotypic characterization of alveolar monocyte recruitment in acute respiratory distress syndrome. Am J Physiol Lung Cell Mol Physiol 2000, 279: L25
2. Zwadlo, G et al; A monoclonal antibody to a differentiation antigen present on human macrophages and absent from monocytes. J Immunol 1985, 134: 1487
3. Kiefer, R et al; Macrophage differentiation antigens in acute and chronic autoimmune polyneuropathies. Brain 1998, 121: 469
4. Zwadlo, G et al; A monoclonal antibody to a novel differentiation antigen on human macrophages associated with the down-regulatory phase of the inflammatory process. Exp Cell Biol 1987, 55: 295
Storage and stability Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease Cardiology and metabolism, Infectious diseases
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登,从1994开始制造检测抗体和蛋白质,主要侧重与病理学诊断有关的全套产品研发,既有纯化的重组蛋白和多肽,也有单抗和多抗,是世界一流的先天免疫领域的研究试剂制造商。