Hycult Biotech/LOX-1, Human, mAb 23C11/HM2138/100 µg
商品编号：
HM2138
品牌：
hycultbiotech
市场价：
¥9288.00
美元价：
5572.80
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:LOX-1,Human,mAb23C11
Themonoclonalantibody23C11recognizesoxidizedlow-densitylipoproteinreceptor-1(LOX-1).LOX-1isasingle-passtypeIImembraneprotein(~45kDa)andbelongstotheC-typelectin-likeproteinsuperfamily.LOX-1isexpressedathighlevelinendothelialcellsandvascular-richorganssuchasplacenta,lung,liver,brainaorticintima,bonemarrow,spinalcordandsubstantianigra.Itisalsoexpressedonthesurfaceofdendriticcells.ThisuniquescavengerreceptorLOX-1playsimportantrolesinatherogenesis.LOX-1mediatestherecognition,internalizationanddegradationofoxidativelymodifiedlowdensitylipoprotein(oxLDL)byvascularendothelialcells.OxLDLisaMarkerofatherosclerosis,inducingvascularendothelialcellactivationanddysfunction,resultinginpro-inflammatoryresponses,pro-oxidativeconditionsandapoptosis.LOX-1associateswithoxLDLinducingtheactivationofNF-kappa-Bthroughanincreasedproductionofintracellularreactiveoxygenandavarietyofpro-atherogeniccellularresponsesincludingareductionofnitricoxide(NO)release,monocyteadhesionandapoptosis.InadditiontobindingoxLDL,LOX-1actsasareceptorfortheHSP70proteininvolvedinantigencross-presentationtonaiveT-cellsindendriticcells,therebyparticipatingincell-mediatedantigencross-presentation.LOX-1isinvolvedintheinflammatoryprocess,byactingasaleukocyte-adhesionmoleculeatthevascularinterfaceinendotoxin-inducedinflammation.LOX-1alsoactsasareceptorforadvancedglycationend(AGE)products,activatedplatelets,monocytes,apoptoticcellsandbothGram-negativeandGram-positivebacteria.TheLOX-1geneisaso-calledimmediateearlygenethatisdynamicallymodulatedbyseveralfactorsinvitroandinvivo.LOX-1expressionisinducedbystimulisuchasinflammatorycytokines,OxLDL,TNF-alpha,TGF-beta,andANGIIinvitro,andseveralproatherogenicfactorsinvivo.Monoclonalantibody23C11neutralizesLOX-1andinhibitsHsp70bindingtodendriticcellsandHsp70-inducedantigencross-presentation.Invivo,targetingLOX-1withatumorantigenusinganti-LOX-1antibody23C11inducesanti-tumorimmunity.
Specifications:
| Catalognumber | HM2138 |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 100µg |
| Formulation | 1ml(100µg/ml)0.2µmfilteredantibodysolutioninPBS,containing0.1%bovineserumalbumin. |
| Application | Flowcytometry,Functionalstudies,Immunofluorescence |
| ApplicationNotes | FC:10 µg/mlantibodyusedonhumanperipheralbloodmyeloidDCandmacrophagesFS:antibodytotallypreventedHsp70bindingtoLOX-1-CHObutnotmock-transfectedCHOcells(Ref1) |
| Use | Forflowcytometrydilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.Forfunctionalstudies,invitrodilutionshavetobeoptimizedinuser"sexperimentalsettting. |
| Immunogen | Fusionproteinofextracellulardomain(aa71-273)ofhumanLOX-1withmurineFcγ1(LOX-1-muFc)producedinPEAKcells |
| Isotype | MouseIgG1 |
| Species | Human |
| Crossreactivity | Mouse-Yes |
| Alias | Lectin-likeoxidizedLDLreceptor1,Ox-LDLreceptor1,C-typelectindomainfamily8memberA |
| PositiveControl | HumanAPCs |
| References | 1.Delneste,Yetal;InvolvementofLOX-1indendriticcell-mediatedantigencross-presentation.Immunity2002,17:353 2.Jeannin,Petal;ComplexityandcomplementarityofoutermembraneproteinArecognitionbycellularandhumoralinnateimmunityreceptors.Immunity2005,22:551 3.Parlato,Setal;LOX-1asanaturalIFN-;-mediatedsignalforapoptoticcelluptakeandantigenpresentationinedndriticcells.Blood2010,115:1554 |
| StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseorderivationofthisproduct. |
| Disease | Cardiologyandmetabolism |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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