Hycult Biotech/CD46, Human, mAb M177, FITC/HM2103F/100 µg
商品编号：
HM2103F
品牌：
hycultbiotech
市场价：
¥10320.00
美元价：
6192.00
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description: CD46, Human, mAb M177, FITC
The monoclonal antibody M177 recognizes CD46, also designated membrane cofactor protein (MCP). CD46 is a 45-70 kDa protein with genetic and tissue-specific heterogeneity.- It is expressed on every cell and tissue, with the exception of erythrocytes. CD46 serves to inhibit complement activation on host tissue. It performs this function by serving as a cofactor which binds to C3b and C4b. This binding is permitted by factor I, a serine protease of plasma, to degrade C3b and C4b and serves to protect the host cell against autologous attack. It also serves as a receptor for measles virus. Four isoforms of CD46 predominate and arise by alternative splicing of a single CD46 gene. CD46 cDNA encodes a signal sequence followed by four complement control protein domains (also called short consensus repeats (SCR)). The monoclonal antibody M177 reacts with the SCR2 domain.
Specifications:
| Catalog number | HM2103F |
|---|---|
| Product type | Monoclonal antibodies |
| Quantity | 100 µg |
| Formulation | 1 ml (100 μg/ml) 0.2 μm protein G purified FITC conjugated antibody solution in PBS, containing 1% bovine serum albumin and 0.02% sodium azide. |
| Conjugate | FITC |
| Application | Flow cytometry, Frozen sections, Functional studies, Immuno assays, Immuno precipitation, Western blot |
| Application Notes | FC: Antibody M177 stains the extracellular domain of CD46. IP: 107 cells were lysed and immunoprecipitated with 25 µg M177 antibody and 25 µg protein G-sepharose W: A non-reduced sample treatment and SDS-page was used. The band size is 45-70 kDa (Ref 1 and 2). |
| Use | Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting. |
| Immunogen | Purified MCP |
| Isotype | Mouse IgG1 |
| Species | Human |
| Alias | Membrane cofactor protein, TLX, trophoblast leucocyte common antigen Gene name: CD46 |
| Positive Control | CD46 is expressed on every cell and tissue |
| Negative Control | Erythrocytes |
| References | 1. Seya, T et al; Quantitative analysis of membrane cofactor protein (MCP) of complement. High expression of MCP on human leukemia cell lines, which is down-regulated during cell differentiation. J Immunol 1990, 145: 238 2. Iwata, K et al; Diversity of sites for measles virus binding and for inactivation of complement C3b and C4b on membrane cofactor protein CD46. J Biol Chem 1995, 270: 15148 3. Kurita-Taniguchi, M et al; Functional modulation of human macrophages through CD46 (Measles virus receptor): production of IL-12 p40 and nitric oxide in association with recruitment of protein-tyrosine phosphatase SHP-1 to CD46. J Immunol 2000, 165: 5143 4. Kurita-Taniguchi, M et al; Molecular assembly of CD46 with CD9, alpha3-beta1 integrin and protein tyrosine phosphatase SHP-1 in human macrophages through differentiation by GM-CSF. Mol Immunol 2001, 38: 689 5. Loré, K et al; Myeloid and plasmacytoid dentritic cells are susceptible to recombinant adenovirus vectors and stimulate polyfunctional memory T cell responses. J Immunol 2007, 179: 1721 6. Adams, W et al; Attenuation of CD4+ T-cell function by human adenovirus type 35 is mediated by the knob protein. J Gen Virol 2012, 93:1339 7. Johnson, M et al; Type I IFN Induced by Adenovirus Serotypes 28 and 35 Has Multiple Effects on T Cell Immunogenicity. J Immunol 2012, 188:6109 8. Teigler, J et al; Vaccination with Adenovirus Serotypes 35, 26, and 48 Elicits Higher Levels of Innate Cytokine Responses than Adenovirus Serotype 5 in Rhesus Monkeys. J Virol 2012, 86:9590 9. Abbink, P et al; Construction and Evaluation of Novel Rhesus Monkey Adenovirus Vaccine Vectors. J Virol 2015, 89:1512 10. Fujiyuki, T et al; A measles virus selectively blind to signaling lymphocytic activation molecule shows anti-tumor activity against lung cancer cells. Oncotarget 2015, 6:24895 |
| Storage and stability | Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year. |
| Precautions | For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product. |
| Disease | Infectious diseases, Tumor immunology |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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