Hycult Biotech/JAM-A, Human, mAb M.Ab.F11/HM2099/100 µg
商品编号：
HM2099
品牌：
hycultbiotech
市场价：
¥9288.00
美元价：
5572.80
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:JAM-A,Human,mAbM.Ab.F11
ThemonoclonalantibodyM.Ab.F11recognizesjunctionaladhesionmolecule-A(JAM-A)alsoknownasthehumanplateletF11-Receptor(F11R)orJAM-1.JAM-Aisasurfaceglycoproteinduplex(32and35kDa)belongingtotheimmunoglobulinsuperfamilyfoundonthesurfaceofhumanplateletsandatintercellularjunctionsofendothelialcellsandepithelialcells.JAM-AbelongstogetherwithJAM-C(JAM-2)andJAM-B(VE-JAMorJAM-3)toafamilyofadhesionproteinswithaV-C2immunoglobulindomainorganization.JAM-Aplaysanimportantroleintightjunctionswhereitisinvolvedincell-to-celladhesionthroughhomophilicinteractions.Itco-distributeswithothertightjunctioncomponentssuchasZO-1,7H6antigen,cingulinandoccludin.Moreover,JAM-Aplaysaroleinplateletaggregation,secretion,adhesion,spreADIng.
Intheplatelet,JAM-Aisamembraneproteininvolvedin2distinctprocessesinitiatedontheplateletsurface.Namely,,antibody-inducedplateletaggregationandsecretionbothdependentonFcgammaRIIandGPIIb/IIIaintegrin,aprocessthatmaybeinvolvedinpathophysiologicalprocessesassociatedwithcertainthrombocytopeniasandsecondly,antibodymediatedplateletadhesionindependentfromFcgammaRIIor-fibrinogenreceptorthatappearstoplayaroleinphysiologicalprocessesassociatedwithplateletadhesionandaggregation.AphysiologicalrolefortheJAM-Aproteinwasdemonstratedbyitsphosphorylationafterthestimulationofplateletsbythrombinandcollagen.ApathophysiologicalrolefortheJAM-AwasrevealedbydemonstratingthepresenceofJAM-Aantibodiesinpatientswiththrombocytopenia.AdhesionofplateletsthroughJAM-Aresultedineventscharacteristicoftheactionofcelladhesionmolecules.RecentdatasuggestsaroleforJAM-Aintheadhesionofplateletstocytokine-inflamedendothelialcellsandthusinthrombosisandatherosclerosisinducedinnon-denudedbloodvesselsbyinflammatoryprocesses.
Intheplatelet,JAM-Aisamembraneproteininvolvedin2distinctprocessesinitiatedontheplateletsurface.Namely,,antibody-inducedplateletaggregationandsecretionbothdependentonFcgammaRIIandGPIIb/IIIaintegrin,aprocessthatmaybeinvolvedinpathophysiologicalprocessesassociatedwithcertainthrombocytopeniasandsecondly,antibodymediatedplateletadhesionindependentfromFcgammaRIIor-fibrinogenreceptorthatappearstoplayaroleinphysiologicalprocessesassociatedwithplateletadhesionandaggregation.AphysiologicalrolefortheJAM-Aproteinwasdemonstratedbyitsphosphorylationafterthestimulationofplateletsbythrombinandcollagen.ApathophysiologicalrolefortheJAM-AwasrevealedbydemonstratingthepresenceofJAM-Aantibodiesinpatientswiththrombocytopenia.AdhesionofplateletsthroughJAM-Aresultedineventscharacteristicoftheactionofcelladhesionmolecules.RecentdatasuggestsaroleforJAM-Aintheadhesionofplateletstocytokine-inflamedendothelialcellsandthusinthrombosisandatherosclerosisinducedinnon-denudedbloodvesselsbyinflammatoryprocesses.
Specifications:
| Catalognumber | HM2099 |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 100µg |
| Formulation | 1ml(100µg/ml)0.2µmfilteredantibodysolutioninPBS,containing0.1%bovineserumalbumin. |
| Application | Flowcytometry,Functionalstudies,Immunoassays,Immunofluorescence,Immunoprecipitation,Paraffinsections,Westernblot |
| ApplicationNotes | FC:AntibodyM.Ab.F11stainstheextracellulardomainofJAM-Aproteinname.Cellswereincubatedwith5 µg/mlofmAbin0.1%BSA/PBS.Asnegativecontrolanisotype-matchedantibodywasused(Ref.4) W:Anon-reducedandreducedsampletreatmentandSDS-Pagewasused.Thebandsizes-are32and35kDa(Ref.1). FS:AntibodyM.Ab.F11functionsasanagoNISTresultinginplateletaggregationatconcentrationsofapproximately5 µg/ml(Ref.1). |
| Use | Forimmunohistochemistry,flowcytometryandWesternblotting,dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.Forfunctionalstudies,dilutionshavetobeoptimizedinuser"sexperimentalsetting. |
| Immunogen | Humanplateletmembranes |
| Isotype | MouseIgG1 |
| Species | Human |
| Alias | JAM-1,plateletF11receptor,F11R,plateletadhesionmolecule,CD321 |
| References | 1.Kornecki,Eetal;Activationofhumanplateletsbyastimulatorymonoclonalantibody.JBiolChem1990,265:10042 2.Naik,Uetal;Mechanismsofplateletactivationbyastimulatoryantibody:cross-linkingofanovelplateletreceptorformonoclonalantibodyF11withtheFcgammaRIIreceptor.BiochemJ1995,310:155 3.Wang,Fetal;Stimulatoryantibody-inducedactivationandselectivetranslocationofproteinkinaseCisoenzymesinhumanplatelets.BiochemJ1995,311:401 4.Sobocka,Metal;CloningofthehumanplateletF11receptor:acelladhesionmoleculememberoftheimmunoglobulinsuperfamilyinvolvedinplateletaggregation.Blood2000,95:2600 5.BABInska,Aetal;TworegionsofthehumanplateletF11-receptor(F11R)arecriticalforplateletaggregation,potentiationandadhesion.ThrombHaemost2002,87:712 6.Babinska,Aetal;TheF11receptor(F11R/JAM-A)inatherothrombosis:overexpressionofF11Rinatheroscleroticplaques.ThrombHaemost2007,97:272 7.Cavusoglu,Eetal;AssociationofplasmalevelsofF11receptor/junctionaladhesionmolecule-A(F11R/JAM-A)withhumanatherosclerosis.JACC2007,50:1768 8.Koenen,Retal;RegulatedreleaseandfunctionalmodulationofjunctionaladhesionmoleculeAbydisintegrinmetalloproteinases.Blood2009,113:4799 |
| Storageandstability | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforatleastoneyear.Theexactexpirydateisindicatedonthelabel. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
| Disease | Cardiologyandmetabolism,Tumorimmunology |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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