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Hycult Biotech/TLR9, Human, mAb 5G5, biotinylated/HM2088/50 µg
Hycult Biotech/TLR9, Human, mAb 5G5, biotinylated/HM2088/50 µg
商品编号：
HM2088
品牌：
hycultbiotech
市场价：
¥5184.00
美元价：
3110.40
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:TLR9,Human,mAb5G5,biotinylated
Themonoclonalantibody5G5recognizeshumanToll-likereceptor9.Toll-likereceptors(TLRs)arehighlyconservedfromDrosophilatohumansandsharestructuralandfunctionalsimilarities.TLRsconstituteofafamilyofpatternrecognitionreceptors(PRRs)thatmediatecellularresponsestoalargevarietyofpathogens(viruses,bacteria,andparasites)byspecificrecognitionofso-called‘pathogen-associatedmolecularpatterns’.ActivationofTLRs,afamilyofatleast11differentmembersthatfunctioneitherashomo-orheterodimers,leadstoactivationofNFκB-dependentandIFNregulatoryfactor-dependentsignalingpathways.TLRshaveacentralroleininnateimmunityandarealsorequiredforthedevelopmentofanadaptiveimmuneresponse.TLRsareexpressedbyvariouscellsoftheimmunesystem,suchasmacrophagesanddendriticcells.Theyrecognizeandrespondtomoleculesderivedfrombacterial,viralandfungalpathogens.
WhereasmostTLRsareexpressedonthecellsurface,TLR9isexpressedintracellularlywithinoneormoreendosomalcompartmentsandrecognizesnucleicacids.TLR9detectsarathersubtledifferenceintheDNAofvertebratescomparedwiththatofpathogens.VertebrategenomicDNAshavemostlymethylatedCpGdinucleotideswherebacterialandviralDNAshaveunmethylatedCpGdinucleotides.TLR9undergoesrelocationfromendoplasmicreticulumtoCpG-ODN-containingendosomes.IntheseendosomesTLR9becomesafunctionalreceptorafterproteolyticcleavage.TLR9existsasapreformedhomodimerandCpG-ODNbindingpromotesitsconformationalchange,bringingthecytoplasmicTIR-likedomainsclosetoeachother.ThisallowsarecruitmentofthekeyadapterproteinMyD88whichinitiatesasignallingcascade.TheonlyhumanimmunecelltypesknowntoconstitutivelyexpressTLR9andtobeactivatedbyCpGODNarepDCsandBcells.TLR9triggeringinducesanactivationphenotypeintheBcellsandpDCs,characterizedbytheexpressionofcostimulatorymolecules,resistancetoapoptosis,andinducesTh1-typeimmuneresponseprofiles.
WhereasmostTLRsareexpressedonthecellsurface,TLR9isexpressedintracellularlywithinoneormoreendosomalcompartmentsandrecognizesnucleicacids.TLR9detectsarathersubtledifferenceintheDNAofvertebratescomparedwiththatofpathogens.VertebrategenomicDNAshavemostlymethylatedCpGdinucleotideswherebacterialandviralDNAshaveunmethylatedCpGdinucleotides.TLR9undergoesrelocationfromendoplasmicreticulumtoCpG-ODN-containingendosomes.IntheseendosomesTLR9becomesafunctionalreceptorafterproteolyticcleavage.TLR9existsasapreformedhomodimerandCpG-ODNbindingpromotesitsconformationalchange,bringingthecytoplasmicTIR-likedomainsclosetoeachother.ThisallowsarecruitmentofthekeyadapterproteinMyD88whichinitiatesasignallingcascade.TheonlyhumanimmunecelltypesknowntoconstitutivelyexpressTLR9andtobeactivatedbyCpGODNarepDCsandBcells.TLR9triggeringinducesanactivationphenotypeintheBcellsandpDCs,characterizedbytheexpressionofcostimulatorymolecules,resistancetoapoptosis,andinducesTh1-typeimmuneresponseprofiles.
Specifications:
| Catalognumber | HM2088 |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 50µg |
| Formulation | 0.5ml(100µg/ml)0.2µmfilteredantibodysolutioninPBS,containing0.02%sodiumazideand0.1%bovineserumalbumin. |
| Conjugate | Biotin |
| Application | Flowcytometry,Frozensections,Immunoassays,Immunofluorescence,Paraffinsections,Westernblot |
| ApplicationNotes | F:10 µmsectionswerefixedwithacetonefor10minutes.PBSwashedsectionswereincubatedwith5G51:100in1%BSAfor30minutesatRT.(Ref.7) IF:cellswerefixedwith2%formalinfor15minutesatRTandpermeABIlizedwithamAb(4 µg/400 µl)containingbuffer(PBS,0.2%BSA,0.2%saponin)-for1hour.(Ref.1) FC:RAW264.7cellswerefixedfor15minuteswith4%formalinandpermeabilized(PBS,0.5%BSA,0,5%saponin)atRT.(Ref.1) P:paraffinembeddedtissues5 µm;msectionsweremade.Afterantigenretrieval(0.01mol/l,pH6sodiumcitrate)andquenchingofendogenousperoxidase,sectionswereblockedwith0.5%ovalbuminand0.1%gelatinfor20minutesatRT.Sectionswereincubatedwith5G5for1hourat37 °C.(Ref.3) W:reducedlysateswereresolvedby10%SDS-PAGEandblottedonnitrocellulose.Afterblockingwith5%skimmedmilkTLR9wasdetectedwith2 µg/ml5G5.(Ref.1) |
| Use | Forimmunohistology,flowcytometryandWesternblottingdilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
| Immunogen | Purified-fusionproteinofextracellulardomainofhumanTLR9(AA1-815)andhumanIgGFc |
| Isotype | MouseIgG2a |
| Species | Human |
| Crossreactivity | Canine-Yes,Mouse-Yes |
| Alias | CD289,TLR9 |
| References | 1.Ahmad-Nejad,Petal;BacterialCpG-DNAandlipopolysaccharidesactivateToll-likereceptorsatdistinctcellularcompartments.EurJImmunol2002,32:1958 2.Rutz,Metal;Toll-likereceptor9bindssingle-strandedCpG-DNAinasequence-andpHdependentmanner.EurJImmunol2004,34:2541 3.RumioCetal;DegranulationofPanethcellsviaToll-likereceptor9.AmJPathol2004,165:373 4.Pratesi,Getal;TherapeuticsynergismofgemcitabineandCpG-oligodeoxynucleotidesinanorthotopichumanpancreaticcarcinomaxenograft.Cancerres2005,65:6388 5.Tokumasa,Netal;ExpressionofTyk2indendriticcellsisrequiredforIl-2,Il-23,andIFNgproductionandtheinductionofTh1celldifferentiation.Blood2007,110:553 6.Burgener,Ietal;AntibodiesspecificforhumanormurineToll-likereceptorsdetectcanineleukocytesbyflowcytometry.VetImmunolImmunopathol2008,124;184 7.Machida,Hetal;ExpressionofToll-likereceptor9inrenalpodocytesinchildhood-onsetactiveandinactivelupusnephritis.NephrolDialTransplant2010,25:2530 8.Zheng,Wetal.Distincthost-relateddendriticcellresponsesduringtheearlystageofPlasmodiumyoeliiinfectioninsusceptIBLeandresistantmice.Parasiteimmunology2010,32:324 |
| Storageandstability | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsibleforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
| Disease | Infectiousdiseases |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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