Hycult Biotech/TLR1，人，单克隆抗体GD2.F4，生物素化/HM2086/50µg
商品编号：
HM2086
品牌：
hycultbiotech
市场价：
¥4896.00
美元价：
2937.60
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:TLR1,Human,mAbGD2.F4,biotinylated
ThemonoclonalantibodyGD2.F4reactswithhumanTLR1.Toll-likereceptors(TLR)arehighlyconservedthroughoutevolutionandplayanessentialroleinrecognizingconservedmotifsfoundinvariouspathogensandinitiatinganappropriateinnateimmuneresponse.Inhuman,tenmembersoftheTLRfamilyhavebeenidentifiedastypeItransmembranesignalingreceptorscontainingmultiplecopiesofleucinerichrepeatsintheextracellulardomainandaninterleukin-1(IL-1)receptormotifinthecytoplasmicdomain.MammalianresponsivenesstomicrobialproductsmaybemediatedbycombinationsofTLRs,forexampleaco-operativeeffectisobservedbetweenTLR1andTLR2-inresponsetobacteriallipoproteins.Ontheotherhand,TLR1wasshowntohavethecapacitytoabrogateTLR4signaling.Ingeneral,TLR1isexpressedathigherlevelsascomparedtootherTLRs.ThehighestexpressionofTLR1isfoundinmonocytesbutitcanalsobeexpressedbymacrophages,dendriticcells,B,T,andNKcells.Inrecentstudies,severalhumanTLR1polymorphismshavebeenassociatedwithimpairedmycobacterialsignalingandsusceptibilitytotuberculosis.
Specifications:
| Catalognumber | HM2086 |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 50µg |
| Formulation | 0.5ml(100µg/ml)0.2µmfilteredantibodysolutioninPBS,containing0.1%bovineserumalbuminand0.02%sodiumazide. |
| Conjugate | Biotin |
| Application | Flowcytometry,Frozensections,Functionalstudies,Immunoassays,Immunofluorescence,Paraffinsections |
| ApplicationNotes | FC:AntibodyGD2.F4stainstheextracellulardomainofTLR1.MonocyteswereresUSPendedinPBS,0.1%BSA,0.02%NAN3containing15 µg/mlGD2.F4.AsnegativecontrolanIgG1isotypecontrolwasused(Ref.1). IHC-P:TissuesectionswerepretreatedwithTargetRetrievalsolution,1%TritonX-100toimprovemembranepermeABIlityand0.03%hydrogenperoxidetoquenchendogenousperoxidases.Tissuesectionswereblockedwith2%FCS(Ref.6). IHC-F:-Tissuesectionswereblockedwithnormalhorseserumpriortostaining(Ref.4). FS:AntibodyGD2.F4ewasusedtoinhibitcytokineproductionofstimulatedPBMCs(Ref.3). |
| Use | Forflowcytometryandimmunohistochemistrydilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.Forfunctionalstudies,dilutionshavetobeoptimizedinuser’sexperimentalsetting. |
| Immunogen | HeLacellstransfectedwithTLR1mRNA(Ref.1) |
| Isotype | MouseIgG1 |
| Species | Human |
| Alias | CD281,toll-likereceptor1,TLR1Fc |
| References | 1.Wyllie,Detal;EvidenceforanaccessoryproteinfunctionforToll-likereceptor1inanti-bacterialresponses.JImmunol2000,165:7125 2.Visintin,Aetal;RegulationofToll-likereceptorsinhumanmonocytesanddendriticcells.JImmunol2001,166:249 3.Sandor,Fetal;Importanceofextra-andintracellulardomainsofTLR1andTLR2inNFkBsignaling.JCB2003,162:1099 4.Ochoa,Metal;DistributionofToll-likereceptor1andToll-likereceptor2inhumanlymphoidtissue.Immunology2003,108:10 5.Ritter,Metal;CharacterizationofToll-likereceptorsinprimarylungepithelialcells:strongimpactoftheTLR3ligandpoly(I:C)ontheregulationofToll-likereceptors,adaptorproteinsandinflammatoryresponse.JInflamm2005,2:16 6.Månsson,Aetal;AdistinctToll-likereceptorrepertoireinhumantonsillarBcells,directlyactivatedbyPam3CSK4,R-837andCpG-2006stimulation.Immunology2006,118:539 7.Panda,Aetal;Age-associateddecreaseinTLRfunctioninprimaryhumandendriticcellspredictsinfluenzavaccineresponse.JImmunol2010,184:2518 |
| Storageandstability | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforatleastoneyear.Theexactexpirydateisindicatedonthelabel. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
| Disease | Infectiousdiseases |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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