Hycult Biotech/TLR1, Human, mAb GD2.F4, FITC/HM2085F/100 µg
商品编号：
HM2085F
品牌：
hycultbiotech
市场价：
¥7752.00
美元价：
4651.20
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description: TLR1, Human, mAb GD2.F4, FITC
The monoclonal antibody GD2.F4 reacts with human TLR1. Toll-like receptors (TLR) are highly conserved throughout evolution and play an essential role in recognizing conserved motifs found in various pathogens and initiating an appropriate innate immune response. In human, ten members of the TLR family have been identified as type I transmembrane signaling receptors containing multiple copies of leucine rich repeats in the extracellular domain and an interleukin-1 (IL-1) receptor motif in the cytoplasmic domain. Mammalian responsiveness to microbial products may be mediated by combinations of TLRs, for example a co-operative effect is observed between TLR1 and TLR2- in response to bacterial lipoproteins. On the other hand, TLR 1 was shown to have the capacity to abrogate TLR4 signaling. In general, TLR1 is expressed at higher levels as compared to other TLRs. The highest expression of TLR1 is found in monocytes but it can also be expressed by macrophages, dendritic cells, B, T, and NK cells. In recent studies, several human TLR1 polymorphisms have been associated with impaired mycobacterial signaling and susceptibility to tuberculosis.
Specifications:
| Catalog number | HM2085F |
|---|---|
| Product type | Monoclonal antibodies |
| Quantity | 100 µg |
| Formulation | 1 ml (100 μg/ml) 0.2 μm filtered FITC conjugated antibody solution in PBS, containing 1% bovine serum albumin and 0.02% sodium azide. |
| Conjugate | FITC |
| Application | Flow cytometry, Frozen sections, Functional studies, Immuno assays, Immuno fluorescence, Paraffin sections |
| Application Notes | FC: Antibody GD2.F4 stains the extracellular domain of TLR1. Monocytes were resuspended in PBS, 0.1% BSA, 0.02% NAN3 containing 15 µg/ml GD2.F4. As negative control an IgG1 isotype control was used (Ref.1). IHC-P: Tissue sections were pretreated with Target Retrieval solution, 1% Triton X-100 to improve membrane permeability and 0.03% hydrogen peroxide to quench endogenous peroxidases . Tissue sections were blocked with 2% FCS (Ref.6). IHC-F:- Tissue sections were blocked with normal horse serum prior to staining (Ref.4). FS: Antibody GD2.F4e was used to inhibit cytokine production of stimulated PBMCs (Ref.3). |
| Use | For flow cytometry and immunohistochemistry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, dilutions have to be optimized in user’s experimental setting. |
| Immunogen | HeLa cells transfected with TLR1 mRNA (Ref.1) |
| Isotype | Mouse IgG1 |
| Species | Human |
| Alias | CD281, toll-like receptor 1, TLR1Fc |
| References | 1. Wyllie, D et al; Evidence for an accessory protein function for Toll-like receptor 1 in anti-bacterial
responses. J Immunol 2000, 165: 7125 2. Visintin, A et al; Regulation of Toll-like receptors in human monocytes and dendritic cells. J Immunol 2001, 166: 249 3. Sandor, F et al; Importance of extra- and intracellular domains of TLR1 and TLR2 in NFkB signaling. JCB 2003, 162: 1099 4. Ochoa, M et al; Distribution of Toll-like receptor 1 and Toll-like receptor 2 in human lymphoid tissue. Immunology 2003, 108: 10 5. Ritter, M et al; Characterization of Toll-like receptors in primary lung epithelial cells: strong impact of the TLR3 ligand poly(I:C) on the regulation of Toll-like receptors, adaptor proteins and inflammatory response. J Inflamm 2005, 2: 16 6. Månsson, A et al; A distinct Toll-like receptor repertoire in human tonsillar B cells, directly activated by Pam3CSK4, R-837 and CpG-2006 stimulation. Immunology 2006, 118: 539 7. Panda, A et al; Age-associated decrease in TLR function in primary human dendritic cells predicts influenza vaccine response. J Immunol 2010, 184: 2518 |
| Storage and stability | Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label. |
| Precautions | For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product. |
| Disease | Infectious diseases |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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