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Hycult Biotech/LL-37/CAP-18, Human, mAb 1-1C12/HM2071/100 µg
Hycult Biotech/LL-37/CAP-18, Human, mAb 1-1C12/HM2071/100 µg
商品编号：
HM2071
品牌：
hycultbiotech
市场价：
¥10320.00
美元价：
6192.00
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:LL-37/CAP-18,Human,mAb1-1C12
Themonoclonalantibody1-1C12recognizesfreeLL-37aswellasitsprecursorhumanCAP18(hCAP18).hCAP18isacathelicidinproteinof~18kDa.ItbelongstothecathelicidinfamilyofantimicrobialpeptidesthatarecharacterizedbyaconservedN-terminalcathelindomainandavariableC-terminalantimicrobialdomain.Cathelicidinsarepredominantlyfoundintheperoxidase-negativegranulesofneutrophils.Duringbacterialinfections,thelifespanofneutrophilsisregulatedbyvariouspathogen-andhost-derivedsubstances.Withintheneutrophils,thecathelicidinsaresynthesizedaspreproproteins.Afterremovalofthesignalpeptide,theyarestoredingranulesasinactiveproforms.TheC-terminalantimicrobialpeptidesareactivatedwhencleavedfromtheproformsofthecathelicidinsbyserineproteasesfromazurophilgranules.
hCAP18isamajorproteininspecificgranulesofneutrophils,butitisalsopresentinsubpopulationsoflymphocytesandmonocytes,insquamousepithelia(ofthemouth,tongue,esophagus,cervix,andvagina),pulmonaryepithelium,keratinocytesininflammatoryskindiseasesandintheepididymus.TheantibacterialC-terminusofhCAP18,LL-37(37aminoacids),hasbeenshowntoexertbroadantimicrobialactivitytowardsgram-negativeaswellasgram-positivebacteriaandtohavesynergisticantibacterialeffectswiththedefensins.Forinstancedeficiencyinsaliva,LL-37accordswithoccurrenceofperiodontaldiseaseinpatientswithmorbusKostmann.LL-37doesnotonlykillbacteria,butcanalsomodulate(suppress)neutrophilapoptosisviatheactivationofFPRL1andP2X7inbacterialinfections.Suppressionofneutrophilapoptosisresultsintheprolongationoftheirlifespan,andmaybeadvantageousforhostdefenseagainstbacterialinvasion.Moreoveritfunctionsasachemotacticagentforneutrophils,monocytesandTcells.LL-37ismarkedlyresistanttoproteolyticdegradationandtoalimitedextentalsocytotoxictowardsmammaliancells.
hCAP18isamajorproteininspecificgranulesofneutrophils,butitisalsopresentinsubpopulationsoflymphocytesandmonocytes,insquamousepithelia(ofthemouth,tongue,esophagus,cervix,andvagina),pulmonaryepithelium,keratinocytesininflammatoryskindiseasesandintheepididymus.TheantibacterialC-terminusofhCAP18,LL-37(37aminoacids),hasbeenshowntoexertbroadantimicrobialactivitytowardsgram-negativeaswellasgram-positivebacteriaandtohavesynergisticantibacterialeffectswiththedefensins.Forinstancedeficiencyinsaliva,LL-37accordswithoccurrenceofperiodontaldiseaseinpatientswithmorbusKostmann.LL-37doesnotonlykillbacteria,butcanalsomodulate(suppress)neutrophilapoptosisviatheactivationofFPRL1andP2X7inbacterialinfections.Suppressionofneutrophilapoptosisresultsintheprolongationoftheirlifespan,andmaybeadvantageousforhostdefenseagainstbacterialinvasion.Moreoveritfunctionsasachemotacticagentforneutrophils,monocytesandTcells.LL-37ismarkedlyresistanttoproteolyticdegradationandtoalimitedextentalsocytotoxictowardsmammaliancells.
Specifications:
| Catalognumber | HM2071 |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 100µg |
| Formulation | 1ml(100µg/ml)0.2µmfilteredantibodysolutioninPBS,containing0.1%bovineserumalbuminand0.02%sodiumazide. |
| Application | Immunoassays,Immunofluorescence,Paraffinsections,Westernblot |
| ApplicationNotes | IA:1-1c12wasusedasdetectionantibody(1:120indilutionbuffer) IF:paraformaldehydefixedcellswereincubatedwith1-1c12(1:50diluted) P:SectionsweredeparaffinizedwithsodiumcitrateandstainedforLL-37(1:50diluted) W:-Anon-reducedsampletreatmentanddenaturingornativeSDS-PAGEwasapplied.Proteinsweretransferredtonitrocellulose,blockedwith5%drymilkandincubatedwith1-1c12,1:250-1000dilution.Thebandsizesare18and4.5kDaforhCAP-18andLL-37respectively(Ref.1-3) |
| Use | ForWesternblottingdilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
| Immunogen | mixtureofsyntheticnativeandglutaraldehydecross-linkedLL-37<sup>1sup> |
| Isotype | MouseIgG2a |
| Species | Human |
| Alias | AntibacterialproteinLL-37,CathelicidinantimicrobialpeptideCAP18;CRAMP;FALL-39;FALL39;-CAP-18;hCAP-18;HSD26 |
| PositiveControl | Humanneutrophils |
| References | 1.Felgentreff,Ketal;Theantimicrobialpeptidecathelicidininteractswithairwaymucus.Peptides2006,27:3100 2.Bucki,Retal;Salivarymucinsinhibitantibacterialactivityofthecathelicidin-derivedLL-37peptidebutnotthecationicsteroidCSA-13.JAntimicrobChemother2008,62:329 3.Dick,Eetal;Exvivo-expandedbonemarrowCD34+derivedneutrophilshavelimitedbactericidalABIlity.StemCells2008,26:2552 4.VonHaussen,Letal;ThehostdefencepeptideLL-37/hCAP18isagrowthfactorforlungcancercells.LungCanc2008,59:12 5.Kockritz-Blickwede,Metal.Phagocytosis-independentantimicrobialactivityofmastcellsbymeansofextracellulartrapformation.Blood2008.111(6):3070 6.Coffelt,SB.OvariancancersoverexpresstheantimicrobialproteinhCAP-18anditsderivativeLL-37increasesovariancancercellproliferationandinvasion.IntJCancer2008.122:1030-1039 |
| Storageandstability | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
| Disease | Autoimmunity,Infectiousdiseases |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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