Hycult生物技术/脂磷壁酸，单克隆抗体55/HM2048/>200微克
商品编号：
HM2048
品牌：
hycultbiotech
市场价：
¥8232.00
美元价：
4939.20
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:Lipoteichoicacid,mAb55
Themonoclonalantibody55recognizeslipoteichoicacid(LTA).LTA,aglycerolphosphatesurfacepolymer,isacomponentoftheenvelopeofGram-positivebacteria.LTAisanchoredviaitsglycolipidstothemembraneandcarriesapolysaccharidechainextendingintothepeptidoglycanlayerofthecellwall.LTAisreleasedspontaneouslyintotheculturemediumduringgrowthofgram-positivebacteria.LTAfunctionsasanimmuneactivatorwithcharacteristicsverysimilartolipopolysaccharide(LPS)fromGram-negativebacteria.LTAbindstoCD14andtriggersactivationpredominantlyviaToll-likereceptor2.AlthoughLTAisinternalizedandtrafficstotheGolgi,thecellularactivationinresponsetoLTAoccursatthecellsurface.
Specifications:
| Catalognumber | HM2048 |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | >200µg |
| Formulation | 1ml(>200μg/ml)culturemediumwithalowendotoxinlevelcontaining0.02%sodiumazide. |
| Application | Flowcytometry,Frozensections,Immunoassays,Immunofluorescence,Westernblot |
| ApplicationNotes | IA:Fordetection,1.2μg/mlantibodyinPBSwasaddedfor1hrat37°ConLTAcoatedplates(Ref.2,3,8). IF:60’inPBS/0.02%BSA/0.02%saponin(ref4)W:Areducedornativesampletreatmentandrunon15%SDS-Page.Blotwasincubatedo/nat4°Cwitha1/1000dilution.Thebandsizeis~17kDa(Ref.6,7). |
| Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
| Immunogen | MicrobialmixtureofStreptococcussobrimsHG961,HG962,HG970,andHG977(Ref.1) |
| Isotype | MouseIgG3 |
| Species | N/A |
| Alias | LTA |
| PositiveControl | CulturemediumofGram-positivebacteria. |
| NegativeControl | Culturemediumofeukaryoticcells |
| References | 1.Hogg,Setal;Occurrenceoflipotechoicacidinoralstreptococci.Int.journalofsystematicbacteriology1997,47:62 2.Langevelde,Petal;Antibiotic-inducedreleaseoflipoteichoicacidandpeptidoglycanfromStaphylococcusaureus:QuantitativemeasurementsandBIOLOGicalreactivities.AntimicrobAgentsChemother1998,42:30733.Langevelde,Petal;Antibiotic-inducedcellwallfragmentsofStaphylococcusaureusincreaseendothelialchemokinesecretionandadhesivenessforgranulocytes.AntimicrobAgentsChemother1999,43:2984 4.Triantafilou,Metal;Lipoteichoicacidandtoll-likereceptor2internalizationandtargetingtothegolgiarelipidraft-dependent.JBiolChem2004,279:408825.Henneke,Petal;RoleoflipoteichoicacidinthephagocyteresponsetogroupBStreptococcus.JImmunol2005,174:6449 6.Grundling,Aetal.SynthesisofglycerolphosphatelipoteichoicacidinStaphylococcusaureus.PNAS2007,104:84787.Jimenez-Dalmaroni,Metal.SolubleCD36ectodomainbindsnegativelychargeddiacylglycerolligandsandactsasaco-receptorforTLR2.PlosOne2009,4:7411 8.Yajima,Aetal.ContributionofphosphoglucosaminemutasetotheresistanceofStreptococcusgordoniiDL1topolymorphonuclearleukocytekilling.FEMSMicrobiollett2009,297:1969.Hashimoto,Yetal.IdentificationofLTAasaligandfordraperinthephagocytosisofStaphylococcusaureusbyDrosophilahemocytes.I.Immunol2009,183:7451 10.Hirose,Yetal.Lipoteichoicacidsonlactobacillusplatarumcellsurfacescorrelatewithinductionofinterleukin-12p40production.Microbiolimmunol2010,54:14311.Wormann,M.E.etal.EnzymaticactivitiesandfunctionalinterdependenciesofBacillussubtilislipoteichoicacidsynthesisenzymes.MolecularMicrobiology2011,79:566 12.Palomino,M.M.etalOsmoticstressadaptationinLactobacilluscaseiBL23leadstostructuralchangesinthecellwallpolymerlipoteichoicacid.Microbiology2013,159:241613.Tadmor,Ketal.ListeriaMonocytogenesMDRtransportersareinvolvedinLTAsynthesisandtriggeringinnateimmunityduringinfection.Frontiersincellularandinfectionmicrobiology2014,4:16 |
| StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
| Disease | Infectiousdiseases |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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