Hycult Biotech/Beta1 Integrin, Human, mAb BV7/HM2033/100 µg
商品编号：
HM2033
品牌：
hycultbiotech
市场价：
¥7752.00
美元价：
4651.20
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:Beta1Integrin,Human,mAbBV7
ThemonoclonalantibodyBV7recognizeshumanß1-integrin.Beta-1integrinisaubiquitouslyexpressed~89kDatypeItransmembraneproteinfunctioningasreceptorwhenheterodimerizedwithonealphasubunit.Itbelongstotheintegrinbetachainfamilyconsistingoffourdifferentgenes,encodingmultipleβ-integrinsviaalternativesplicing.Ligand-recognitiondependsonthecompositionoftheheterodimer:eithercollagen,fibronectin,VCAM1,laminin,cytotactin,osteopontin,epiligrin,thrombospondinandCSPG4canbindtotheintegrin-complex.Beta-1integrinsrecognizethesequenceR-G-Dinawidearrayofligands.Isoformbeta-1Binterfereswithisoformbeta-1Aresultinginadominantnegativeeffectoncelladhesionandmigration(invitro).IncaseofHIV-1infection,theinteractionwithextracellularviralTatproteinseemstoenhanceangiogenesisinKaposi"ssarcomalesions.Whenassociatedwithα7,β1-integrinregulatescelladhesionandlamininmatrixdeposition.BV7isactiveonHT-29coloncarcinomacellsandonHCCP-2998tumorcells.Itisinvolvedinpromotingendothelialcellmotilityandangiogenesis.FurThermore,β1-integrinplaysamechaNISTicadhesiveroleduringtelophase,andisrequiredforthesuccessfulcompletionofcytokinesis.Uponactivationintegrinsingeneral,includingβ1-integrin,areknowntoexhibitglobalstructuralrearrangementsandexposureofligandbindingsites.β1-integrinmodulationisofimportanceintissuerepairandregeneration.Inculturedprimaryhippocampalneurons,astrocytesandtissues,cellsurfaceexpressionofamyloidbetafibrils(keyhallmarkofAlzheimer’sdisease)selectivelyco-localizedwithβ1-integrin.Preincubationofcellswithantibodiesagainstβ1-integrin,aswellasα1-integrin,greatlyenhancedamyloidbeta-inducedapoptosis,indicatingaprotectiveroleforintegrinsinapoptosis.ThemonoclonalantibodyBV7doesnotrecognizeα5β1complexandnotthecytoplasmicpartoftheβ1-subunit.MonoclonalantibodyBV7isactiveonHT-29coloncarcinomacellsandonHCCP-2998tumorcells.BV7bindstoseveralothertumorcells(MG3osteosarcoma,A375melanoma,MHCC-1410andLovocoloncarcinoma)butdoesnotaffectadhesiontoendothelialcells.
Specifications:
| Catalognumber | HM2033 |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 100µg |
| Formulation | 1ml(100µg/ml)0.2µmfilteredantibodysolutioninPBS,containing0.1%bovineserumalbumin. |
| Application | Flowcytometry,Functionalstudies,Immunoassays,Immunoprecipitation,Westernblot |
| ApplicationNotes | FC:AntibodyBV7stainstheextracellulardomainofbeta-1integrin.HUVECcellswerefixedin4%paraformaldehydebeforestaining.MouseIgG1isotypewasusedasnegative. FS:AntibodyBV7functionsasaninhibitingantibody.Theantibodywasfunctionallytestedbyadhesionassay(ref1),chemotaxis(ref2),patchclampanalysis(ref3)andneurotoxicityinducedapoptosis(ref4). |
| Use | ForflowcytometryandWesternblottingdilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.ForneutralizationofBIOLOGicalactivityinvitrodilutionshavetobemadeaccordingtotheamountsofbeta1integrintobeinactivated. |
| Immunogen | HumanumbilicalveinEC |
| Isotype | MouseIgG1 |
| Species | Human |
| Alias | CD29,fibronectinreceptorsubunitbeta,-integrinVLA-4subunitbeta |
| PositiveControl | HT-29coloncarcinoma,Endothelialcells |
| References | 1.Martin-Padura,Ietal;Anovelmechanismofcoloncarcinomacelladhesiontotheendotheliumtriggeredbyß1integrinchain.JBiolChem1994,269:6124 2.Palmieri,Detal;TrimercarboxylpropeptideofcollagenIproducedbymatureosteoblastsischemotacticforendothelialcells.JBiolChem2000,275:32658 3.Martel,Vetal;Talincontrolsexitoftheintegrinα5β1fromanearlycompartmentofthesecretorypathway.JCellSci2000,113:1951 4.Hofmann,Getal;HERGK+channelsactivationduringβ1integrin-mediatedadhesiontofibronectininducesanup-regulationofαvβ3integrininthepreosteoclasticlekemiacelllineFLG29.1.JBiolChem2001,276:4923 5.Bozzo,Cetal;Involvementofβ1integrininβAP-inducedapoptosisinhumanneuroblastomacells.MolCellNeurosci2004,25:1 |
| StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
| Disease | Autoimmunity,Neurologicaldisorders |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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