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Hycult Biotech/VE-Cadherin, Human, mAb BV9, FITC/HM2032F/100 µg
Hycult Biotech/VE-Cadherin, Human, mAb BV9, FITC/HM2032F/100 µg
商品编号：
HM2032F
品牌：
hycultbiotech
市场价：
¥10320.00
美元价：
6192.00
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:VE-Cadherin,Human,mAbBV9,FITC
ThemonoclonalantibodyBV9bindstotheextracellulardomain(EC3-EC4)ofhumanVE-cadherin(vascularendothelialcadherin).Endothelialcellscontrolthepassageofplasmaconstituentsandcirculatingcellsfrombloodtotheunderlyingtissues.VE-cadherinisofvitalimportanceforthemaintenanceandcontrolofendothelialcellcontacts.MechanismsthatregulateVE-cadherinmediatedadhesionareimportantforthecontrolofvascularpermeABIlityandleukocyteextravasation.VE-cadherinregulatesvariouscellularprocessessuchascellproliferationandapoptosisandmodulatesvascularendothelialgrowthfactorreceptorfunctions.Therefore,VE-cadherinisalsoessentialduringembryonicangiogenesis.ThespecializedfunctionofVE-cadherinislostorimpairedinseveralpathologicalconditions-includinginflammation,sepsis,ischemiaanddiabetes-whichleadstosevere,andsometimesfatal,organdysfunction.FurThermore,abnormalincreaseinvascularpermeabilityisoftenobservedinpathologicalconditions,suchastumor-inducedangiogenesis,maculardegeneration,allergy,andbrainstroke.
Endothelialpermeabilityisregulatedinpartbythedynamicopeningandclosureofcell-celladherentjunctions.Invascularendothelium,adherentjunctionsaremainlycomposedofVE-cadherin,anadhesivereceptorthatisabletoself-associateatendothelialcellcellcontacts.VE-cadherinlinksendothelialcellstogetherbyhomophilicinteractionsmediatedbyitsextracellularpartandassociatesintracellularlywiththeactinCytoskeletonviacatenins.VE-cadherinbelongstothecadherinsuper-familyofcellcelladhesionmolecules,whichareencodedbymorethan200genesinthehumangenome.ClassicalcadherinsareCa2+-dependent,homophilic,celltocelladhesionmoleculesexpressedinnearlyallcellswithinsolidtissues.Cadherinsformacoreadhesioncomplexthatconsistsofacadherindimer,bindingthroughitsextracellularregiontoanotherdimerofcadherinsexpressedinadjacentcells,whileitsintracellularregionisanchoredtotheplasmamembraneandlinkedtothecytoskeleton.TheVE-cadherinextracellulardomainconsistsoffivecadherin-typerepeats,calledEC(extracellularcadherin)domainsthatareboundtogetherbycalciumionsinarod-likestructure.
Endothelialpermeabilityisregulatedinpartbythedynamicopeningandclosureofcell-celladherentjunctions.Invascularendothelium,adherentjunctionsaremainlycomposedofVE-cadherin,anadhesivereceptorthatisabletoself-associateatendothelialcellcellcontacts.VE-cadherinlinksendothelialcellstogetherbyhomophilicinteractionsmediatedbyitsextracellularpartandassociatesintracellularlywiththeactinCytoskeletonviacatenins.VE-cadherinbelongstothecadherinsuper-familyofcellcelladhesionmolecules,whichareencodedbymorethan200genesinthehumangenome.ClassicalcadherinsareCa2+-dependent,homophilic,celltocelladhesionmoleculesexpressedinnearlyallcellswithinsolidtissues.Cadherinsformacoreadhesioncomplexthatconsistsofacadherindimer,bindingthroughitsextracellularregiontoanotherdimerofcadherinsexpressedinadjacentcells,whileitsintracellularregionisanchoredtotheplasmamembraneandlinkedtothecytoskeleton.TheVE-cadherinextracellulardomainconsistsoffivecadherin-typerepeats,calledEC(extracellularcadherin)domainsthatareboundtogetherbycalciumionsinarod-likestructure.
Specifications:
| Catalognumber | HM2032F |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 100µg |
| Formulation | 1ml(100μg/ml)0.2μmfilteredproteinGpurifiedFITCconjugatedantibodysolutioninPBS,containing1.0%bovineserumalbuminand0.02%sodiumazide. |
| Conjugate | FITC |
| Application | Flowcytometry,Frozensections,Functionalstudies,Immunoassays,Immunofluorescence,Immunoprecipitation,Westernblot |
| ApplicationNotes | F:AcetonefixedsectionswereblockedwithhorseserumandincubatedwithantibodyBV9for30minutes(Ref.2). FC:AntibodyBV9stainstheextracellulardomainofVE-cadherin.AsnegativecontrolanIgGisotypecontrolwasused(Ref.4) FS:AntibodyBV9functionsasanantagoNIST.Theantibodywasfunctionallytestedbyadding10-50 µg/mlantibodyBV9tocellculture.TheantibodyblocksVE-cadherincausingaredistributionofVE-cadherinawayfromintracellularjunctions(Ref.5,6). IA:AntibodyBV9canfunctionascoatanddetector. IF:Cellsoncoverslipswerefixedwith3%paraformaldehydeandpermeabilizedwith0.5%TritonX-100beforeincubationwithantibodyBV9(Ref.5,8). W:Areducedsampletreatmentand7.5%SDS-Pagewasused.Thebandsizeis130-140kDa(Ref.3). |
| Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
| Isotype | MouseIgG2a |
| Species | Human |
| Alias | vascularendothelialcadherin,7B4antigen,Cadherin-5,CD144 Genename:CDH5 |
| PositiveControl | HUVECsgrownoncoverslips |
| References | 1.Navarro,Petal;Catenin-dependentand-independentfunctionsofvascularendothelialcadherin.JBiolChem1995,270:30965 2.Martin-Padura,Ietal;-ExpressionofVE(vascularendothelial)-cadherinandotherendothelial-specificMarkersinhaomangiomas.Jpath1995,175:51 3.Breviario,Fetal;Functionalpropertiesofhumanvascularendothelialcadherin(7B4/Cadherin-5),anendothelium-specificcadherin.ArteriosclerThromb1995,15:1229- 4.Gill,Metal.VasculartraumainducesrapidbuttransientmobilizationofVEGFR2+AC133+endothelialprecursorcells.CircRes2001,88:167 5.Spagnuolo,Retal.Gas1isinducedbyVE-cadherinandvascularendothelialgrowthfactorandinhibitsendothelialcellapoptosis.Blood2004,103:3005 6.Kuchler,Aetal.Nuclearil-33isgenerallyexpressedinrestingendotheliumbutrapidlylostuponangiogenicorproinflammatoryactivation.AMJpath.2008,173:1229 7.Abraham,Setal.VE-cadherinmediatedcell-cellinteractionsuppressessproutingviasignallingtoMLC2phosphorylation.2009,19:668 8.Vallon,Metal.Tumorendothelialmarker5expressioninendothelialcellsduringcapillarymarphogenesisisinducesbythesmallGTPaseRacandmediatescontactinhibitionofcellproliferation.ExpCellres.2010,316:412 |
| Storageandstability | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
| Disease | Cardiologyandmetabolism,Tumorimmunology |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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