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当前位置: 首页 > 产品中心 > monoclonal_antibody > Hycult Biotech/IP-10, Human, mAb 6D4, FITC/HM2030F/100 µg
商品详细Hycult Biotech/IP-10, Human, mAb 6D4, FITC/HM2030F/100 µg
Hycult Biotech/IP-10, Human, mAb 6D4, FITC/HM2030F/100 µg
Hycult Biotech/IP-10, Human, mAb 6D4, FITC/HM2030F/100 µg
商品编号: HM2030F
品牌: hycultbiotech
市场价: ¥5184.00
美元价: 3110.40
产地: 美国(厂家直采)
公司:
产品分类: 单克隆抗体
公司分类: monoclonal_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Description:IP-10,Human,mAb6D4,FITC

Themonoclonalantibody6D4recognizeshumanC-X-Cmotifchemokine10(IP-10),aproteinof98aminoacids.IP-10,alsoknownasCXCL10,functionsasligandfortheCXCR3receptor.IP-10belongstotheα-chemokine(C-X-C)family,whichcanbedividedintwosubfamilies:(1)potentchemoattractantsforneutrophils,likeIL-8and(2)potentchemoattractantsforlymphocytes,liketheIFNɣinducIBLeprotein(IP)-10.IP-10isproducedbyawidevarietyofcelltypesrangingfromneutrophils,dendriticcellsandmonocytestohepatocytes,endothelialcellsandkeratinocytes.ThecytokineisreportedtobeinvolvedinascalaofinflammatorypathologiessuchasHIV,encephalitis,cutaneousTcelllymphoma,chronichepatitis,psoriasisandacuteanterioruveitis.VariousobservationsstronglysuggestarolefortheC-X-CchemokinesIL-8andIP-10intheregulationofangiogenicactivityincancerandinidiopathicpulmonaryfibrosis.FurThermoreIP-10isassociatedwithacuterejectionprocessesestimatedbythepredictivepropertiesofurinaryIP-10expressionfortheshort-andlong-termgraftfunctionafterkidneytransplantation.

Specifications:

CatalognumberHM2030F
ProducttypeMonoclonalantibodies
Quantity100µg
Formulation1ml(100μg/ml)0.2μmfilteredproteinGpurifiedFITCconjugatedantibodysolutioninPBS,containing1.0%bovineserumalbuminand0.02%sodiumazide.
ConjugateFITC
ApplicationFlowcytometry,Frozensections,Immunoassays,Immunofluorescence,Westernblot
ApplicationNotesFC:Antibody6D4stainshumanCXCL10.Forintracellularstainingactivatedplasmacytoiddendriticcellswerefixedwith2%paraformaldehydeandpermeABIlizedwith0.5%saponin.Allstepswereperformedinpresenceof0.5MEDTAtoavoidcellaggregation(Ref.3)
W:TissuewashomogenizedinloADIngsamplebufferandsubjectedtoSDS-PAGE.Thebandsizeis~9kDa(Ref.4).
IHC-F:Tissuesectionswerefixed10minutesinacetoneandpretreatedwithavidin/biotinblockingkit.Aspositivecontrolchronicallyinflamedliverwasusedandasnegativecontrolnormalliverandisotypematchedantibody(Ref.4).
UseDilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.
ImmunogenRecombinanthumanIP-10
IsotypeMouseIgG2a
SpeciesHuman
Alias10kDainterferongamma-inducedprotein,CXCL10,Small-induciblecytokineB10
Genename:CXCL10
PositiveControlAcitvatedplasmacytoiddendriticcells
NegativeControlNormalliver
References1.Hamamdzic,D.etal;Reovirustriggerscelltype-specificproinflammatoryresponsesdependentontheautocrineactionofIFNβ.AmJPhysiolLungCellMolPhysiol2001,280:L18
2.Giustizieri,Metal;Nitricoxidedonorssuppresschemokineproductionbykeratinocytesinvitroandinvivo.AmJPath2002,161:14093.Bendriss-Vermare,Netal;VirusoverridesthepropensityofhumanCD40L-activatedplasmacytoiddendriticcellstoproduceTh4mediatorsthroughsynergisticinductionofIFN-gammaandTh1chemokineproduction.JofLeukocBiol.2005,78:954
4.Curbishley,Setal;CXCR3activationpromoteslymphocytetransendothelialmigrationacrosshumanhepaticendotheliumunderfluidflow.AmJPathol2005,167:8875.Wetzel,Metal;μ-OpioidInductionofMonocyteChemoattractantProtein-1,RANTES,andIFN-g-InducibleProtein-10ExpressioninHumanPeripheralBloodMononuclearCells.JImmunol2000,165:6519
StorageandstabilityProductshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforatleastoneyear.Theexactexpirydateisindicatedonthelabel.
PrecautionsForresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsibleforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct.
DiseaseInfectiousdiseases
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登,从1994开始制造检测抗体和蛋白质,主要侧重与病理学诊断有关的全套产品研发,既有纯化的重组蛋白和多肽,也有单抗和多抗,是世界一流的先天免疫领域的研究试剂制造商。