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Hycult Biotech/TNF-RI, Human, mAb H398, biotinylated/HM2021/50 µg
Hycult Biotech/TNF-RI, Human, mAb H398, biotinylated/HM2021/50 µg
商品编号：
HM2021
品牌：
hycultbiotech
市场价：
¥6960.00
美元价：
4176.00
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:TNF-RI,Human,mAbH398,biotinylated
ThemonoclonalantibodyH398recognizestheextracellularpartoftheTumorNecrosisFactorReceptortypeI(TNF-RI)ofthemembrane-boundaswellasthesolublereceptor.TNF-RI(~55-60kDa)ispresentonmostcelltypesandisconsideredtoplayaprominentroleincellstimulationbyTNF-alpha.TNF-alphaactivatesinflammatoryresponses,inducesapoptosis,regulatescellularproliferation,andmayevenpromotecancerprogression.TheeffectsofTNF-alphaaremediatedbyTNF-RIandTNF-RII,whichhavebothdistinctandoverlappingdownstreamsignalingcascades.InductionofcytotoxicityandotherfunctionsaremediatedlargelyviaTNF-RI.TNF-RIisequallywellactivatedbyboththe17kDasolubleand26kDamembrane-boundform,whereasTNF-RIIisefficientlyactivatedonlybythemembraneboundformofTNF-alpha.
TNF-RIsignalingisinitiatedwhentrimericTNF-alphabindsTNF-RIreceptors.SubsequentTNF-RItrimerizationpromotestherecruitmentofaproximalsignalingcomplexcomposedofTNFReceptorAssociatedproteinwithaDeathDomain(TRADD),ReceptorInteractingProtein(RIP),cellularInhibitorofApoptosisProtein1(cIAP1),TNFReceptorAssociatedFactor2(TRAF2),andlikelyTRAF5.StudieswithTNF-RI-deficientmiceindicatethatTNF-RImediatesmostoftheproliferation,pro-inflammatory,andapoptosis-activatingpathways.
TNF-RIsignalingisinitiatedwhentrimericTNF-alphabindsTNF-RIreceptors.SubsequentTNF-RItrimerizationpromotestherecruitmentofaproximalsignalingcomplexcomposedofTNFReceptorAssociatedproteinwithaDeathDomain(TRADD),ReceptorInteractingProtein(RIP),cellularInhibitorofApoptosisProtein1(cIAP1),TNFReceptorAssociatedFactor2(TRAF2),andlikelyTRAF5.StudieswithTNF-RI-deficientmiceindicatethatTNF-RImediatesmostoftheproliferation,pro-inflammatory,andapoptosis-activatingpathways.
Specifications:
| Catalognumber | HM2021 |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 50µg |
| Formulation | 0.5ml(100µg/ml)0.2μmfilteredproteinGpurifiedbiotinylatedantibodysolutioninPBS,containing0.1%bovineserumalbuminand0.02%sodiumazide. |
| Conjugate | Biotin |
| Application | Flowcytometry,Functionalstudies,Immunoassays,Immunoprecipitation,Westernblot |
| ApplicationNotes | W:Reducedsampleconditionswereused.Abandsizeof~50-60kDaisexpected. FC:AntibodyH398canbeusedforextracellularstaining. |
| Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.TheantibodycompeteswithTNF-alpha. |
| Immunogen | Affinity-purifiedreceptormaterialfromHL60cellscontaining~9μgactivelybindingreceptor(Ref.1). |
| Isotype | MouseIgG2a |
| Species | Human |
| Crossreactivity | Rat-Yes |
| Alias | CD120a,Tumornecrosisfactorreceptorsuperfamilymember1A,Tumornecrosisfactorreceptor1,p55,p60,TNFR-1 Genename:TNFRSF1A,TNFAR,TNFR1 |
| PositiveControl | THP-1cells |
| References | 1.Thoma,Betal;Identificationofa60kDtumornecrosisfactor(TNF)receptorasthemajorsignaltransducingcomponentinTNFresponses.JExpMed1990,172:1019 2.Grell,Metal;TR60andTR80tumornecrosisfactor(TNF)-receptorscanindependentlymediatecytolysis.LymphokineCytokineRes1993,12:143 3.Scheurich,Petal;AgoNISTicandantagonisticantibodiesasatooltostudythefunctionalroleofhumantumornecrosisfactorreceptors.TumorNecrosisfactor1993,4:52 4.Grell,Metal;Thetype1receptor(CD120a)isthehigh-affinityreceptorforsolubletumornecrosisfactor.ProcNatlAcadSciUSA1998,95:570 5.Krippner-Heidenreich,Aetal;Single-chainTNF,aTNFderivativewithenhancedstABIlityandantitumoralactivity.JImmunol2008,180:8176 6.Kontermann,Retal;Ahumanizedtumornecrosisfactorreceptor1(TNFR1)-specificantagonisticAntibodyforselectiveinhibitionoftumornecrosisfactor(TNF)action.JImmunother2008,31:225 7.Kälble,Fetal;SelectiveBlockingofTNFReceptor1AttenuatesPeritonealDialysisFluidInducedinflammationofthePeritoneuminMice.PLoSONE2016,11:e0163314 |
| Storageandstability | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseorderivationofthisproduct. |
| Disease | Autoimmunity,Gastroenterology |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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