Hycult Biotech/gC1qR, Human, mAb 60.11/HM2014/100 µg
商品编号：
HM2014
品牌：
hycultbiotech
市场价：
¥6432.00
美元价：
3859.20
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:gC1qR,Human,mAb60.11
Themonoclonalantibody60.11recognizesacellmembraneC1qbindingmoleculethatrecognisestheglobularheadsofC1q.Itisalsopresentinplasmaandtheextracellularmatrix.Themoleculeisanunusuallyacidic,singlechainproteinwithanapparentmolecularweightof33kDa.ItdoesnotpossessaconventionalsequencemotifcompatIBLewithamembranespanningsegmentnoraconsensussiteforaGPIanchor.gC1q-Rmigratesasasinglechainunderbothreducingandnon-reducingconditions,butitbehavesasanoligomerongel-filtrationinnon-dissociatingconditions.ItsmultimerformationmaybeacriticalprocessbywhichthegC1q-RmoleculeincreasesitsaffinityformultivalentligandssuchasC1q.
gC1q-RhasbeenshowntoinhibitcomplementactivationbypreventingthebindingofC1qtoantibodies,suggestingthatthebindingsiteforgC1q-Randthebindingsiteforimmunecomplexes,whicharepresentontheC1qglobular‘heads’,maybelocatedatthesameposition.gC1q-Riscapableofinteractingwithseveralproteinsinvolvedinbloodclotting,namely,thrombin,prothrombin,theheparinbindingformofvitronectin,theternarycomplex,vitronectin-thrombin-antithrombin,aswellashigh-molecular-weightkininogenandHagemanfactor.Besidesitsroleinthecomplementpathway,gC1q-RparticipatesinenhancementofFc-receptorandCR1-mediatedphagocytosis,procoagulantactivityonplatelets,andchemotacticactivityonmastcells,eosinophils,neutrophils,andfibroblasts.gC1q-Risexpressedonawidevarietyofcellsandcanserveasabindingsiteforplasmaandmicrobialproteins.ItsantigenicsitesmaybecrypticoncellsinsUSPensionbutbecomeexposedwhenthecellsarefixedbybifunctionalcross-linkers.Probablyitisalsoexpressedonthecellmembraneasatetramer.CrosslinkingoractivationmaythusbringaboutatetramericassemblyofgC1q-Rfollowedbyaconformationalchangewithinthemolecule,therebyexposingepitopeswhichareotherwisehidden.AformofGC1q-Risalsofoundinsidethecell.IntracellulargC1q-Rhasbeenshowntobindthecytoplasmictailoftheα1B-adrenergicreceptorandtoPKCμ.
Themonoclonalantibody60.11isdirectedagainstepitopessituatedwithintheNH2-terminalstretchofgC1q-Rcorrespondingtoresidues76-93.Clone60.11recognizestheputativeC1qbindingsiteandreactswiththematureform,buthaspoorornoreactivitywiththetruncatedform,lackingresidues74-95.
gC1q-RhasbeenshowntoinhibitcomplementactivationbypreventingthebindingofC1qtoantibodies,suggestingthatthebindingsiteforgC1q-Randthebindingsiteforimmunecomplexes,whicharepresentontheC1qglobular‘heads’,maybelocatedatthesameposition.gC1q-Riscapableofinteractingwithseveralproteinsinvolvedinbloodclotting,namely,thrombin,prothrombin,theheparinbindingformofvitronectin,theternarycomplex,vitronectin-thrombin-antithrombin,aswellashigh-molecular-weightkininogenandHagemanfactor.Besidesitsroleinthecomplementpathway,gC1q-RparticipatesinenhancementofFc-receptorandCR1-mediatedphagocytosis,procoagulantactivityonplatelets,andchemotacticactivityonmastcells,eosinophils,neutrophils,andfibroblasts.gC1q-Risexpressedonawidevarietyofcellsandcanserveasabindingsiteforplasmaandmicrobialproteins.ItsantigenicsitesmaybecrypticoncellsinsUSPensionbutbecomeexposedwhenthecellsarefixedbybifunctionalcross-linkers.Probablyitisalsoexpressedonthecellmembraneasatetramer.CrosslinkingoractivationmaythusbringaboutatetramericassemblyofgC1q-Rfollowedbyaconformationalchangewithinthemolecule,therebyexposingepitopeswhichareotherwisehidden.AformofGC1q-Risalsofoundinsidethecell.IntracellulargC1q-Rhasbeenshowntobindthecytoplasmictailoftheα1B-adrenergicreceptorandtoPKCμ.
Themonoclonalantibody60.11isdirectedagainstepitopessituatedwithintheNH2-terminalstretchofgC1q-Rcorrespondingtoresidues76-93.Clone60.11recognizestheputativeC1qbindingsiteandreactswiththematureform,buthaspoorornoreactivitywiththetruncatedform,lackingresidues74-95.
Specifications:
| Catalognumber | HM2014 |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 100µg |
| Formulation | 1ml(100µg/ml)0.2µmfilteredantibodysolutioninPBS,containing0.1%bovineserumalbumin. |
| Application | Flowcytometry,Functionalstudies,Immunoassays,Immunoprecipitation,Paraffinsections,Westernblot |
| Use | ForflowcytometryandWesternblotting,dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.Forfunctionalstudies,invitrodilutionshavetobeoptimizedinuser"sexperimentalsetting. |
| Immunogen | Recombinant-gC1q-RcorrespondingtomaturegC1q-R(aminoacids74-282) |
| Isotype | MouseIgG1 |
| Species | Human |
| Crossreactivity | Rat-Yes,Syrianhamster-Yes |
| Alias | GlycoproteingC1qBP,GC1q-Rprotein,hyaluronan-bindingprotein1,mitochondrialmatrixproteinp32,p33 |
| References | 1.Ghebrehiwet,Betal;IdentificationoffunctionaldomainsongC1Q-R,acellsurfaceproteinthatbindstotheglobular"heads"ofC1Q,usingmonoclonalantibodiesandsyntheticpeptides.Hybridoma1996,5:333 2.Ghebrehiwet,Betal;EvidencethatthetwoC1qbindingmembraneproteins,gC1q-RandcC1q-R,associatetoformacomplex.JImmunol1997,159:1429 3.Ghebrehiwet,Betal;gC1q-R/p33,amemberofanewclassofmultifunctionalandmulticompartmentalcellularproteins,isinvolvedininflammationandinfection.ImmunolRev2001,180:65 4.Grace,Ketal;SurfaceexpressionofcomplementreceptorgC1q-R/p33isincreasedontheplasmamembraneofhumanspermatozoaaftercapacitation.BiolReprod2002,66:823 5.Peerschke,Eetal;gC1qR/p33blockadereducesStaphylococcusaureuscolonizationoftargettissuesinananimalmodelofinfectiveendocarditis.InfectImmun2006,74:4418 6.Sansonno,Detal;RoleofthereceptorfortheglobulardomainofC1qproteininthepathogenesisofhepatitisCvirau-relatedcryoglobulinvasculardamage.JImmunol2009,183:6013 |
| StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsibleforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
| Disease | Cardiologyandmetabolism,Tumorimmunology |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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