Hycult Biotech/TNF-alpha, Human, mAb 52B83/HM2010/100 µg
商品编号：
HM2010
品牌：
hycultbiotech
市场价：
¥6960.00
美元价：
4176.00
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:TNF-alpha,Human,mAb52B83
Themonoclonalantibody52B83reactswithtumornecrosisfactoralpha(TNF-alpha).TNF-alphaisahomotrimeric17kDaprotein,thatinteractswitheitheroneofthetwotypesofTNF-receptors,termedIandII,leADIngtoreceptorcross-linkingandsignaltransduction.Thereceptorsdifferstronglyintheirintra-cellularsignalingpathways.
TNF-alphawasoriginallydescribedasahighlycytotoxiccytokinefortumorcells,itcausestumornecrosisinvivoandshowscytolyticactivityagainsttumorcellsinvitro.FurThermore,-TNF-alphaisfoundtobeacentralmediatorinmanyinflammatoryandimmunologicalprocesses.Itcanbeinducedbyvariousproductsofmicro-organismsandbyvariouscytokinesleadingtoexpressionofawidevarietyof-cytokines.Thepro-inflammatorypropertiesof-TNF-alphaplayacentralroleinseveralauto-immunediseasessuchasrheumatoidarthritisandinhibitionbyneutralizingmoleculeshavebeenshowntobebeneficialinpatients.
TNF-alphawasoriginallydescribedasahighlycytotoxiccytokinefortumorcells,itcausestumornecrosisinvivoandshowscytolyticactivityagainsttumorcellsinvitro.FurThermore,-TNF-alphaisfoundtobeacentralmediatorinmanyinflammatoryandimmunologicalprocesses.Itcanbeinducedbyvariousproductsofmicro-organismsandbyvariouscytokinesleadingtoexpressionofawidevarietyof-cytokines.Thepro-inflammatorypropertiesof-TNF-alphaplayacentralroleinseveralauto-immunediseasessuchasrheumatoidarthritisandinhibitionbyneutralizingmoleculeshavebeenshowntobebeneficialinpatients.
Specifications:
| Catalognumber | HM2010 |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 100µg |
| Formulation | 1ml(100μg/ml)0.2μmfilteredproteinGpurifiedantibodysolutioninPBS,containing0.1%bovineserumalbuminand0.02%sodiumazide. |
| Application | Flowcytometry,Frozensections,Immunoassays,Westernblot |
| ApplicationNotes | FC:Antibody52B83wasusedtostainsolubleTNFboundtoTNFreceptors.CellswerefixedinPBAcontaining0.2%formaldehyde(Ref.3) W:Areducedsampletreatmentand15%SDS-Pagewasused.Thebandsizeobservedis17kDa(Ref.4). IHC-F:6-μmtissuesectionswerefixedinacetonefor10minutes(Ref.4). |
| Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
| Isotype | MouseIgG1 |
| Species | Human |
| Crossreactivity | Guineapig-Yes,Mouse-Yes,Rhesusmonkey-Yes |
| Alias | TumorNecrosisFactor-alpha,TNF,TNF-SF2,cachectin |
| References | 1.Bradding,Petal;Interleukin-4,-5,and-6andTumorNecrosisFactor-alphainnormalandasthmaticairways:Evidenceforthehumanmastcellasasourceofthesecytokines.AmJRespirCellMolBiol1994,10:471 2.Bradding,Petal:TNFalphaislocalizedtonasalmucosalmastcellsandisreleasedinacuteallergicrhinitis.ClinExpAllergy1995,25:406 3.Gerspach,Jetal;Detectionofmembrane-boundTumorNecrosisFactor(TNF):ananalysisofTNF-specificreagents.MicroscResTech2000,50:243 4.Laanvander,Netal;Tumornecrosisfactoralpha(TNFalpha)inhumanskin:acomparisonofdifferentantibodiesforimmunohistochemistry.ArchDermatolRes2001,293:226 5.Lee,Eetal;Clinicalandimmunohistochemicalcharacteristicsofmucoceles.AnnDermatol2009,21:345 6.Brunner,Petal;InfliximabinducesdownregulationoftheIL-12/IL-23axisin6-sulfo-LacNac(slan)1dendriticcellsandmacrophages.JAllergyClinImmunol2013,132:1184 |
| StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
| Disease | Autoimmunity,Gastroenterology |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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