Hycult Biotech/IFN-α，小鼠，重组（CHO衍生）/HC1040-10/10E6单位
商品编号：
HC1040-10
品牌：
hycultbiotech
市场价：
¥9080.00
美元价：
5448.00
产品分类：
其它重组蛋白
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:IFN-alpha,Mouse,Recombinant(CHO-derived)
ThishighlyactiveinterferonisconstitutivelyproducedbyChineseHamsterOvary(CHO)cellswhichhavebeentransfectedwiththehybridinterferongenealpha14-16(alpha1AA1-22andalpha2AA23-166).Thehybridalpha1,alpha4geneproductdiffers6aminoacidsfromalpha4(AA5,7,10,17,19and20).TheAA10,17,19and20influencetheantiviralresponse.BIOLOGicalactivityhasbeendemonstratedinmouseaswellasinhamsterandratsystems.Thenaturalalpha4interferonexhibitsantiviralactivityinmousecellswhichisapproximatelysixtimeshigherthanthatexhibitedbynaturalalpha1interferon.Theactivityoftherecombinantproductisincreasedtoalevelwhichisapproximately100timesthatofnaturalalpha1interferonand15timesthatofnaturalalpha4interferon.Thesituationissomewhatdifferentinhamstercells.Here,thenaturalalpha1interferonisabout100timesmoreactivethanthenaturalalpha4interferon.Therecombinantproductisasactiveasthealpha1interferon.
Theabovementionedfeaturesaremostprobablyduetothefactthatatleasttwodifferentreceptorsexistforalphainterferonsinthesesystems.Onereceptorappearstobespecificfortheaminoacids10-20whereastheotherisspecificfortheaminoacids55-67.Therecombinantproductexposesoraccessesbothsitesbetter,oratleastaswellastheparentproteins.Inadditiontotheinterferonalphaproteins"ABIlitytoprotectcellsagainstviralinfection,theycanalsoinhibitcellgrowthandpossessimmunomodulatoryactivities.Theseeffectsaremediatedthroughbindingtoaspecificcellsurfacereceptor.MostoftheinterferonalphaspeciesarenotglycosylatedandarefurthercharacterizedbytheiracidstabilityatpH2.
Oneunitofmousealphainterferonistheamountofalphainterferonwhichprotects50%oftheindicatorcellpopulationfromviraldestruction.OneunitofHycultBiotechMouseAlphaInterferonapproximatesthebioactivitycontainedinoneunitofthemousealphainterferonstandardpreparedbyNIH(Ga02-901-511).
Theabovementionedfeaturesaremostprobablyduetothefactthatatleasttwodifferentreceptorsexistforalphainterferonsinthesesystems.Onereceptorappearstobespecificfortheaminoacids10-20whereastheotherisspecificfortheaminoacids55-67.Therecombinantproductexposesoraccessesbothsitesbetter,oratleastaswellastheparentproteins.Inadditiontotheinterferonalphaproteins"ABIlitytoprotectcellsagainstviralinfection,theycanalsoinhibitcellgrowthandpossessimmunomodulatoryactivities.Theseeffectsaremediatedthroughbindingtoaspecificcellsurfacereceptor.MostoftheinterferonalphaspeciesarenotglycosylatedandarefurthercharacterizedbytheiracidstabilityatpH2.
Oneunitofmousealphainterferonistheamountofalphainterferonwhichprotects50%oftheindicatorcellpopulationfromviraldestruction.OneunitofHycultBiotechMouseAlphaInterferonapproximatesthebioactivitycontainedinoneunitofthemousealphainterferonstandardpreparedbyNIH(Ga02-901-511).
Specifications:
| Catalognumber | HC1040-10 |
|---|---|
| Producttype | Proteins |
| Quantity | 10E6units |
| Species | Mouse |
| Alias | Interferon-alpha,IFN-a |
| Formulation | LyophilizedproductinPBS,100μgmousealbuminand0.1%gelatincontaining1.0x106Units.Reconstitutethevialbypipetting1mldistilledorde-ionizedwater(Caution:vialisundervacuum). |
| Use | Fordilutionsuseproteinstabilizedphosphatebufferedsaline,pH8(donotusefreshserum). |
| Application | Functionalstudies,Immunoassays |
| Storageandstability | Lyophilizedproductshouldbestoredat4°C.Storestocksolutionafterreconstitutioninaliquotsat-20°C.Repeatedfreezeandthawcyclesandprolongedexposuretolightwillcauselossofactivity.Underrecommendedstorageconditions,productisstableforoneyear. |
| References | 1.Katahira,Metal;CytokineregulationoftheratproopiomelanocortingeneexpressioninAtT-20cells.Endocrinology1998,139:2414 2.Fink,Ketal;EarlytypeIinterferon-mediatedsignalsonBcellsspecificallyenhanceantiviralhumoralresponses.EurJImmunol2006,36:2094 3.Hinson,Eetal;ViperinIsHighlyInducedinNeutrophilsandMacrophagesduringAcuteandChronicLymphocyticChoriomeningitisVirusInfection.JImmunol2010,184:5723 4.Rudd,Petal;InterferonResponseFactors3and7ProtectagainstChikungunyaVirusHemorrhagicFeverandShock.JVirol2012,86:9888 5.Schuessler,Aetal;WestNileVirusNoncodingSubgenomicRNAContributestoViralEvasionoftheTypeIInterferon-MediatedAntiviralResponse.JVirol2012,86:5708 6.Sriram,Uetal;MyeloidDendriticCellsfromB6.NZMSle1/Sle2/Sle3Lupus-ProneMiceExpressanIFNSignatureThatPrecedesDiseaseOnset.Jimmunol2012,189:80 |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
| Disease | Infectiousdiseases,Tumorimmunology |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
联络我们
