Hycult Biotech/VAP-1, Mouse, mAb 7-88/HM1094/100 µg
商品编号：
HM1094
品牌：
hycultbiotech
市场价：
¥10320.00
美元价：
6192.00
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:VAP-1,Mouse,mAb7-88
Themonoclonalantibody7-88recognizesmouseVascularAdhesionProtein-1(VAP-1)which-isaglycosylatedhomodimericmembraneproteinconsistingoftwo90kDasubunitsconnectedbydisulfidebonds.ItcontainsashortN-terminalcytoplasmictail,asinglemembrane-spanningdomainandalargeextracellularpart.AsolubleformofVAP-1(sVAP-1)hasbeendescribed,whichpresumablyresultsfromtheproteolyticcleavageofmembrane-boundVAP-1.StructurallyVAP-1belongstoenzymescalledsemicarbamizide-sensitiveamineoxidases,whichcontaincopperasacofactor.Theseenzymesdeaminateprimaryaminesinareactionproducinghydrogenperoxide,aldehyde,andammonia.
VAP-1isexpressedinendothelialcells,smoothmusclecells,ADIpocytes,andinfolliculardendriticcells.InendothelialcellsthemajorityofVAP-1isstoredwithinintracellulargranulesandtranslocatedtothesurfaceuponinflammationwhereitregulatesleukocytetissueinfiltration.FurThermore,theend-productsformedbyVAP-1canalsoregulateleukocytemigrationbysignalingeffects,haveinsulin-likeeffectsinenergymetabolism,andcancausevasculardamagebydirectcytotoxicity.
In-whiteadiposetissueofobeseanddiabeticdb-/-miceincreasedexpressionofVAP-1hasbeenobservedsuggestingthatitcontributes-tothearthrosclerosisandvasculardysfunctionobservedinthesediseases.Moreover,inhibitionofVAP-1reducedtheaccumulationofmyeloidcellsintotumorsandattenuatestumorgrowth.
Themonoclonalantibody7-88inhibitsmigrationofgranulocytesandmonocytesinacutemodelsofinflammation.
VAP-1isexpressedinendothelialcells,smoothmusclecells,ADIpocytes,andinfolliculardendriticcells.InendothelialcellsthemajorityofVAP-1isstoredwithinintracellulargranulesandtranslocatedtothesurfaceuponinflammationwhereitregulatesleukocytetissueinfiltration.FurThermore,theend-productsformedbyVAP-1canalsoregulateleukocytemigrationbysignalingeffects,haveinsulin-likeeffectsinenergymetabolism,andcancausevasculardamagebydirectcytotoxicity.
In-whiteadiposetissueofobeseanddiabeticdb-/-miceincreasedexpressionofVAP-1hasbeenobservedsuggestingthatitcontributes-tothearthrosclerosisandvasculardysfunctionobservedinthesediseases.Moreover,inhibitionofVAP-1reducedtheaccumulationofmyeloidcellsintotumorsandattenuatestumorgrowth.
Themonoclonalantibody7-88inhibitsmigrationofgranulocytesandmonocytesinacutemodelsofinflammation.
Specifications:
| Catalognumber | HM1094 |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 100µg |
| Formulation | 1ml(100µg/ml)0.2µmfilteredantibodysolutioninPBS,containing0.1%bovineserumalbumin |
| Application | Flowcytometry,Frozensections,Functionalstudies,Immunofluorescence,Immunoprecipitation |
| ApplicationNotes | FC:Antibody7-88stainstheextracellulardomainofmouseVAP-1inCHOcellstransfectedwithmouseVAP-1CDNA..Aspositivecontrol-anti-VAP-1clone7-106wasusedandasnegativecontrolanisotype-matchedcontrolantibody.(Ref.2) IHC-F:-Tissuesectionswerefixedinacetoneandincubatedwithantibody7-88for20minutesatroomtemperature..Asnegativecontrolanirrelevantisotype-matchedantibodywasused(Ref.2). FS:Antibody7-88(200 µm)wasintravenouslyinjectedwhichresultedintheinhibitionofleukocytetraffickingininflamedperitoneum.-(Ref.2). |
| Use | Forimmunohistochemistryandflowcytometry,dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.Forfunctionalstudies,dilutionshavetobeoptimizedinuser’sexperimentalsetting. |
| Immunogen | Vesselsfrommouselymphnodes |
| Isotype | RatIgG2b |
| Species | Mouse |
| Alias | Amineoxidasecoppercontaining3;AOC3;SSAO;-membranecopperamineoxidase |
| PositiveControl | MouseVAP-1-transfectedCHOcells(ref.2) |
| References | 1.Abella,Aetal;AdipocytesreleaseasolubleformofVAP-1/SSAObyametalloproteasedependentprocessandinaregulatedmanner.Diabetologia2004,47:429 2.Merinen,Metal;Vascularadhesionprotein-1isinvolvedinbothacuteandchronicinflammationinthemouse.AmJPath2005,166:793 3.Bonder,Cetal;RulesofrecruitmentforTh1andTh4lymphocytesininflamedliver:aroleforalpha-4integrinandvascularadhesionprotein-1.Immunity2005,23:153 4.Marttila-Ichihara,Fetal;Small-moleculeinhibitorsofvascularadhesionprotein-1reducetheaccumulationofmyeloidcellsintotumorsandattenuatetumorgrowthinmice.JImmunol2010,184:3164 5.Iffiú-Soltész,Zetal;Increasedprimaryamineoxidaseexpressionandactivityinwhiteadiposetissueofobeseanddiabeticdb-/-mice.JNeuralTransm2011,DOI:10.1007/s00702-011-0586-9 |
| StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforatleastoneyear.Theexactexpirydateisindicatedonthelabel. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
| Disease | Cardiologyandmetabolism,Nephrology |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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