Hycult Biotech/巨噬细胞F4/80，小鼠，单克隆抗体BM8，FITC/HM1066F/100µg
商品编号：
HM1066F
品牌：
hycultbiotech
市场价：
¥7752.00
美元价：
4651.20
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:MacrophagesF4/80,Mouse,mAbBM8,FITC
ThemonoclonalantibodyBM8recognisesa125kDextracellularmacrophagemembranemolecule,highlyrestrictedtomaturemacrophagesubpopulationsresidingintissue.ThismurineF4/80glycoproteincontainsseven-transmembrane(TM7)regions,whichanchortheproteininthecellmembrane,andtherebyshowssimilarityinthisregiontoG-protein-coupledreceptors.TheF4/80moleculesharesoverallstructuralhomologytoothermembersoftheepidermalgrowthfactor(EGF)-TM7family.Theantigenisdetectedontissuefixedmacrophagesinallorganstestedsofar(spleen,lymphnodes,thymus,liver,skin).ItisalsopresentonLangerhanscellsintheskinandKupffercellsintheliver.Itisabsentongranulocytes,lymphocytesandtrombocytes.TheexpressionofF4/80increasesuponmaturationofmacrophageprecursorsinbonemarrowandbloodaswellasinontogeny.
ThemonoclonalantibodyBM8istheonlymacrophageMarkerthatisabletodistinguishnon-destructivefromdestructiveinflammationprocessesinthepancreas.FurThermoreitisauniquehistologicalmarkeroftheprogressionfromperi-insulitistobeta-celldestructionanddiabetesinamousediabetesmodel.ThemonoclonalantibodyBM8crossreactswithhumanheartmacrophages.
ThemonoclonalantibodyBM8istheonlymacrophageMarkerthatisabletodistinguishnon-destructivefromdestructiveinflammationprocessesinthepancreas.FurThermoreitisauniquehistologicalmarkeroftheprogressionfromperi-insulitistobeta-celldestructionanddiabetesinamousediabetesmodel.ThemonoclonalantibodyBM8crossreactswithhumanheartmacrophages.
Specifications:
| Catalognumber | HM1066F |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 100µg |
| Formulation | 1ml(100μg/ml)0.2μmfilteredproteinGpurifiedFITCconjugatedantibodysolutioninPBS,containing1.0%bovineserumalbuminand0.02%sodiumazide. |
| Conjugate | FITC |
| Application | Flowcytometry,Frozensections,Paraffinsections,Westernblot |
| ApplicationNotes | W:Mousebone-marrowderivedmacrophages;non-reduced;~125kDa(Ref1);reductionwith2-mercaptoethanoldestroysBM8antigen. F:tissueembeddedinOCTTissueTec;fixedwithacetonefor10minatRT;incubationwith0.02MsodiumazideinPBScontaining0.1%H2O2for10minatRTtodestroyendogenousperoxidase;spleenaspositivecontrol.P:fixationin10%neutralbufferedformalinfor24h;blockingwithnon-immunizedgoatserum;microwavedfor6minincitratebuffer;splenicmacrophagesaspositivecontrol(Ref3). FC:fixedwith1%paraformaldehyde(Ref1). |
| Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
| Immunogen | BALB/cmacrophagesobtainedfrom14-day-oldbonemarrowcellcultures |
| Isotype | RatIgG2a |
| Species | Mouse |
| PositiveControl | Mousemacrophages |
| NegativeControl | Mousefibroblastsorgranulocytes |
| References | 1.Malorny,Uetal;Amonoclonalantibodyagainstanantigenpresentonmousemacrophagesandabsentfrommonocytes.CellTissueRes1986,243:421 2.Leenen,Petal;Markersofmousemacrophagedevelopmentdetectedbymonoclonalantibodies.JImmunolMethods1994,174:5 3.Mackler,Aetal;Macrophagetraffickingintheuterusandcervixprecedesparturitioninthemouse.BiolReprod1999,61:879 4.Schaller,Eetal;InactivationoftheF4/80glycoproteininthemousegermline.MolCellBiol2002,22:8035 |
| StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
| Disease | Cardiologyandmetabolism,Infectiousdiseases |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
联络我们
