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当前位置: 首页 > 产品中心 > monoclonal_antibody > Hycult Biotech/巨噬细胞F4/80,小鼠,单克隆抗体BM8/HM1066/100µg
商品详细Hycult Biotech/巨噬细胞F4/80,小鼠,单克隆抗体BM8/HM1066/100µg
Hycult Biotech/巨噬细胞F4/80,小鼠,单克隆抗体BM8/HM1066/100µg
Hycult Biotech/巨噬细胞F4/80,小鼠,单克隆抗体BM8/HM1066/100µg
商品编号: HM1066
品牌: hycultbiotech
市场价: ¥6432.00
美元价: 3859.20
产地: 美国(厂家直采)
公司:
产品分类: 单克隆抗体
公司分类: monoclonal_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Description: Macrophages F4/80, Mouse, mAb BM8

The monoclonal antibody BM8 recognises a 125 kD extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. This murine F4/80 glycoprotein contains seven-transmembrane (TM7) regions, which anchor the protein in the cell membrane, and thereby shows similarity in this region to G-protein-coupled receptors. The F4/80 molecule shares overall structural homology to other members of the epidermal growth factor (EGF)-TM7 family. The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. It is absent on granulocytes, lymphocytes and trombocytes. The expression of F4/80 increases upon maturation of macrophage precursors in bone marrow and blood as well as in ontogeny.
The monoclonal antibody BM8 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas. Furthermore it is a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model. The monoclonal antibody BM8 cross reacts with human heart macrophages.

Specifications:

Catalog number HM1066
Product type Monoclonal antibodies
Quantity 100 µg
Formulation 1 ml (100 μg/ml) 0.2 μm filtered protein G purified FITC conjugated antibody solution in PBS, containing 1.0% bovine serum albumin and 0.02% sodium azide.
Application Flow cytometry, Frozen sections, Paraffin sections, Western blot
Application Notes W: Mouse bone-marrow derived macrophages; non-reduced; ~125 kDa (Ref 1); reduction with 2-mercaptoethanol destroys BM8 antigen.
F: tissue embedded in OCT Tissue Tec; fixed with acetone for 10 min at RT; incubation with 0.02 M sodium azide in PBS containing 0.1 % H2O2 for 10 min at RT to destroy endogenous peroxidase; spleen as positive control.
P: fixation in 10% neutral buffered formalin for 24 h; blocking with non-immunized goat serum; microwaved for 6 min in citrate buffer; splenic macrophages as positive control (Ref 3).
FC: fixed with 1 % paraformaldehyde (Ref 1).
Use Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Immunogen BALB/c macrophages obtained from 14-day-old bone marrow cell cultures
Isotype Rat IgG2a
Species Mouse
Positive Control Mouse macrophages
Negative Control Mouse fibroblasts or granulocytes
References 1. Malorny, U et al; A monoclonal antibody against an antigen present on mouse macrophages and absent from monocytes. Cell Tissue Res 1986, 243: 421
2. Leenen, P et al; Markers of mouse macrophage development detected by monoclonal antibodies. J Immunol Methods 1994, 174: 5
3. Mackler, A et al; Macrophage trafficking in the uterus and cervix precedes parturition in the mouse. Biol Reprod 1999, 61: 879
4. Schaller, E et al; Inactivation of the F4/80 glycoprotein in the mouse germ line. Mol Cell Biol 2002, 22: 8035
Storage and stability Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease Cardiology and metabolism, Infectious diseases
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登,从1994开始制造检测抗体和蛋白质,主要侧重与病理学诊断有关的全套产品研发,既有纯化的重组蛋白和多肽,也有单抗和多抗,是世界一流的先天免疫领域的研究试剂制造商。