Hycult Biotech/MPO，小鼠，单克隆抗体8F4，生物素化/HM1051BT/50µg
商品编号：
HM1051BT
品牌：
hycultbiotech
市场价：
¥6960.00
美元价：
4176.00
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:MPO,Mouse,mAb8F4,biotinylated
Themonoclonalantibody8F4recognizesmousemyeloperoxidase(MPO).MPOisaglycoproteinproducedasasingleprecursor,whichissubsequentlycleavedintoaalphaandbetachain.InhumantheBIOLOGicallyactiveMPOisa150kDatetramercomposedof2glycosylatedalfachainsof59-64kDaand2betachainsof14kDa.MPOisstoredinazurophilicgranulesofpolymorphonuclearleukocytesandisrapidlyreleasedintothephagosomeandextracellularspaceduringinflammatoryconditions.Theenzymecatalyzestheconversionofchlorideandhydrogenperoxidetohypochlorite,apotentoxidant,whichfunctionsinhostdefenseagainstmicroorganisms.
InvolvementofMPOhasbeendescribedinseveralhumandiseases,suchascardiovasculardisease,airwayinflammation,lungcancer,Alzheimer"sdiseaseandmultiplesclerosis.ApositivecorrelationbetweenelevatedMPOlevelsinserumandcardiovasculardiseasesuggestaninterestingroleforMPOasandiagnosticMarker,makingitpossIBLetoidentifypatientsatriskforfuturecardiacevents.FurThermore,therearesomeautoimmunediseases,inwhichMPOistargetedbyantineutrophilcytoplasmantibodies.StudieswithMPO-knockoutmicehaveshownanincreasedsusceptibilitytopneumoniafollowingintratrachealinfections.Moreover,MPOdeficientmicearemoresusceptibletoexperimentalautoimmuneencephalitis,aTcell-dependentneuronaldisease,andhaveanincreasedexpressionofarterioscleroticplaquescomparedtowild-typemice.Theanti-mouseMPOmonoclonalantibody8F4recognizesnaturalMPOinbiologicalsolutionsbyELISA,infrozentissuesectionsfixedwithacetoneandinflowcytometryusingacellpermeABIlizationmethod.
InvolvementofMPOhasbeendescribedinseveralhumandiseases,suchascardiovasculardisease,airwayinflammation,lungcancer,Alzheimer"sdiseaseandmultiplesclerosis.ApositivecorrelationbetweenelevatedMPOlevelsinserumandcardiovasculardiseasesuggestaninterestingroleforMPOasandiagnosticMarker,makingitpossIBLetoidentifypatientsatriskforfuturecardiacevents.FurThermore,therearesomeautoimmunediseases,inwhichMPOistargetedbyantineutrophilcytoplasmantibodies.StudieswithMPO-knockoutmicehaveshownanincreasedsusceptibilitytopneumoniafollowingintratrachealinfections.Moreover,MPOdeficientmicearemoresusceptibletoexperimentalautoimmuneencephalitis,aTcell-dependentneuronaldisease,andhaveanincreasedexpressionofarterioscleroticplaquescomparedtowild-typemice.Theanti-mouseMPOmonoclonalantibody8F4recognizesnaturalMPOinbiologicalsolutionsbyELISA,infrozentissuesectionsfixedwithacetoneandinflowcytometryusingacellpermeABIlizationmethod.
Specifications:
| Catalognumber | HM1051BT |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 50µg |
| Formulation | 0.5ml(100μg/ml)0.2μmfilteredproteinGpurifiedbiotinylatedantibodysolutioninPBS,containing0.1%bovineserumalbuminand0.02%sodiumazide. |
| Conjugate | Biotin |
| Application | Flowcytometry,Frozensections,Immunoassays,Immunofluorescence |
| ApplicationNotes | IA:theantibodycanbeusedasadetectionantibody. FC:Cellsarefixedwith4%paraformaldehydeandpermeabilizedwith0.1%saponin.IA:HM1051wasusedasacaptureantibody.TheexperimentperformedwasaspecificityexperimentwithdifferentconcentrationsofHM1051. |
| Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
| Immunogen | PurifiedmouseMPOfromWEHI-3cells |
| Isotype | MouseIgG1 |
| Species | Mouse |
| Alias | Myeloperoxidase Genename:Mpo |
| PositiveControl | Neutrophilsisolatedfromdigestedinfarcts |
| NegativeControl | Lymphocytesisolatedfromdigestedinfarcts |
| References | 1.Huugen,Detal;Aggravationofanti-myeloperoxidaseantibody-inducedglomerulonephritisbybacteriallipopolysaccharide:roleoftumornecrosisfactor-alpha.AmJPathol2005,167:47 2.Wang,Detal;C/EBPalphadirectsmonocyticcommitmentofprimarymyeloidProgenitors.Blood2006,108:12233.Matthijsen,Metal;Myeloperoxidaseiscriticallyinvolvedintheinductionoforgandamageafterrenalischemiareperfusion.AmJPathol2007,171:1743 4.Leeuwenvan,Metal;Accumulationofmyeloperoxidase-positiveneutrophilsinatheroscleroticlesionsinLDLR-/-mice.ArteriosclerThrombVascBiol2008,28:845.Nahrendorf,Metal;AnactivatableMRimagingagentreportsmyeloperoxidaseactivityinhealinginfarctsanddetectstheanti-inflammatoryeffectsofatorvastatinonischemia-reperfusioninjurynon-invasively.Circulation2008,117:1153 6.Jiang,Detal.RegulationofGranulocyteandMacrophagePopulationsofMurineBoneMarrowCellsbyG-CSFandCD137Protein.PLoSONE2010,5:e155657.Romero,C.Detal.TheToll-LikeReceptor4AgoNISTMonophosphorylLipidAAugmentsInnateHostResistancetoSystemicBacterialInfection.Infect.Immun.2011,79(9):3576 8.Yamada,Metal.Interferon-gProductionbyNeutrophilsduringBacterialPneumoniainMice.AmJRespirCritCareMed2011,183:1391 |
| Storageandstability | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsibleforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
| Disease | Cardiologyandmetabolism,Pulmonology |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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