Hycult Biotech/JAM-A, Mouse, mAb BV12, FITC/HM1050F/100 µg
商品编号：
HM1050F
品牌：
hycultbiotech
市场价：
¥10320.00
美元价：
6192.00
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:JAM-A,Mouse,mAbBV12,FITC
ThemonoclonalantibodyBV12recognizesjunctionaladhesionmolecule-A(JAM-A),alsoknownasJAM-1andthemouseplateletF11-Receptor(F11R),isacelladhesionmolecule(CAM).JAM-Aisamemberoftheimmunoglobulinsuperfamilyfoundonthesurfaceofmouseplateletsandatintercellularjunctionsofendothelialcellsandepithelialcells.JAM-AbelongstogetherwithJAM-B(VE-JAMorJAM-3)andJAM-C(JAM-2)toafamilyofadhesionproteinswithaV-C2immunoglobulindomainorganization.JAMplaysanimportantroleintightjunctionswhereitisinvolvedincell-to-celladhesionthroughhomophilicinteraction.ItcodistributeswithothertightjunctioncomponentsasZO-1,7H6antigen,cingulinandoccludin.InhumanJAM-Aplaysaroleinplateletaggregation,secretion,adhesionandspreADIng.
Specifications:
| Catalognumber | HM1050F |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 100µg |
| Formulation | 1ml(100μg/ml)0.2μmproteinGpurifiedFITCconjugatedfilteredantibodysolutioninPBS,containing1%bovineserumalbuminand0.02%sodiumazide. |
| Conjugate | FITC |
| Application | Flowcytometry,Immunoassays,Immunofluorescence,Immunoprecipitation,Westernblot |
| ApplicationNotes | IHC-F:sectionswereair-driedovernight,fixedinacetonefor10minutes,andincubatedwith10%normalrabbitserum.SectionswerethenincubatedwithdifferentprimaryAb’s(Ref.3). IP:ConfluentmonolayersofH5VcellswerewashedtwicewithPBSandthenlysedfor30minwithlysisbuffer.Insomeexperiments,beforecelllysis,cellswereincubatedfor2hatroomtemperatureinPBSwith5mMbis(sulfosuccinimidyl)suberate(Ref.4). |
| Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
| Immunogen | H5Vendothelialcells(Ref.1) |
| Isotype | RatIgG2a |
| Species | Mouse |
| Crossreactivity | Human-No |
| Alias | JunctionaladhesionmoleculeA,Junctionaladhesionmolecule1,JAM-1,CD321 Genename:F11r,Jam1,Jcam,Jcam1 |
| References | 1.Martìn-Padura,Ietal;JunctionalAdhesionMolecule,aNovelMemberoftheImmunoglobulinSuperfamilyThatDistributesatIntercellularJunctionsandModulatesMonocyteTransmigration.JCellBiol1998,142:117 2.Bazzoni,Getal;InteractionofjunctionaladhesionmoleculewiththetightjunctioncomponentsZO-1,cingulin,andoccludin.JBiolChem2000,275:20520 3.Cera,Metal;IncreasedDCtraffickingtolymphnodesandcontacthypersensitivityinjunctionaladhesionmolecule-A–deficientmice.JClinInvest2004,114:729 4.Martinez-Estrada,Oetal;Oppositeeffectsoftumornecrosisfactorandsolublefibronectinonjunctionaladhesionmolecule-Ainendothelialcells.AJP-LungCellMolPhysiol2005,288:L1081 5.Baluk,Petal;Functionallyspecializedjunctionsbetweenendothelialcellsoflymphaticvessels.JEM2007,204:2349 6.Murakami,Metal;AbrogationofJunctionalAdhesionMolecule-AExpressionInducesCellApoptosisandReducesBreastCancerProgression.PLoSONE2011,6:e21242 |
| StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforatleastoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
| Disease | Cardiologyandmetabolism,Tumorimmunology |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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