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Hycult Biotech/Mannose receptor, Mouse, mAb MR5D3/HM1049/100 µg
Hycult Biotech/Mannose receptor, Mouse, mAb MR5D3/HM1049/100 µg
商品编号：
HM1049
品牌：
hycultbiotech
市场价：
¥9288.00
美元价：
5572.80
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:Mannosereceptor,Mouse,mAbMR5D3
ThemonoclonalantibodyMR5D3recognizestheMannoseReceptor(MR,alsoknownasCD206),amemberofthevertebrateC-typelectinfamily.MRisapatternrecognitionreceptorthatisinvolvedinbothinnateandadaptiveimmunity.The175kDatransmembraneproteinconsistsof5domains:anamino-terminalcysteine-richregion,afibronectintypeIIrepeat,aseriesofeighttandemlectin-likecarbohydraterecognitiondomains(responsIBLefortherecognitionofmannoseandfucose),atransmembranedomainandanintracellularcarboxy-terminaltail.Thestructureissharedbythefamilyofmultilectinmannosereceptors:thephospholipaseA2-receptor,DEC205andthenovelC-typelectinreceptor(mannosereceptorX).TheMRrecognisesawiderangeofgrampositiveandgramnegativebacteria,yeasts,parasitesandmycobacteria.Moreover,theMRhasalsobeenshowntobindandinternalizetissue-typeplasminogenactivator.
MR’sarepresentonmonocytesanddendriticcells(DC)andarepresumedtoplayaroleininnateandadaptiveimmunity,thelatterviaprocessingbyDC.TheexpressionofMRasobservedinimmunohistologyispresentontissuemacrophages,dendriticcells,asubpopulationofendothelialcells,Kupffercellsandspermcells.TheexpressionofMRonmonocytesincreasesduringcultureandcanbeenhancedbycytokinessuchasIL-4.LabellingofMRexpressingmonocytes/macrophagesincreaseswithprolongedincubationtimeprobablyduetointernalizationoftheMR-antibody-complex.DetectionoftheMRwithanti-MRmonoclonalantibodyMR5D3cansubstitutestainingformannosecontainingprobesaslabeledmannosylatedBSA,atechniquewhichismorecumbersomeandlessspecific.
MR’sarepresentonmonocytesanddendriticcells(DC)andarepresumedtoplayaroleininnateandadaptiveimmunity,thelatterviaprocessingbyDC.TheexpressionofMRasobservedinimmunohistologyispresentontissuemacrophages,dendriticcells,asubpopulationofendothelialcells,Kupffercellsandspermcells.TheexpressionofMRonmonocytesincreasesduringcultureandcanbeenhancedbycytokinessuchasIL-4.LabellingofMRexpressingmonocytes/macrophagesincreaseswithprolongedincubationtimeprobablyduetointernalizationoftheMR-antibody-complex.DetectionoftheMRwithanti-MRmonoclonalantibodyMR5D3cansubstitutestainingformannosecontainingprobesaslabeledmannosylatedBSA,atechniquewhichismorecumbersomeandlessspecific.
Specifications:
| Catalognumber | HM1049 |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 100µg |
| Formulation | 1ml(100µg/ml)0.2µmfilteredantibodysolutioninPBS,containing0.1%bovineserumalbumin |
| Application | Flowcytometry,Frozensections,Functionalstudies,Immunoassays,Immunofluorescence,Immunoprecipitation,Westernblot |
| ApplicationNotes | FC:2%paraformaldehydefixedcellswerelabeledwith10 µg/mlofmAb. IHC-F:Sectionsfixedinethanolor2%paraformaldehydewerepermeABIlizedwithPBS/0.1%tritonX100.AfterquenchingandblockingmAbwasadded(10 µg/ml)andincubatedfor1h IA:mAbwasusedasdetectorat10 µg/mlfor2h. IP:MRwasimmunoprecipiatedusingantibodyMR5D3(10 µg/ml)andGammaBindPlussepharose(Ref.2) W:Clearedlysateswererunon6%SDS-PAGEundernonreducingconditions.AftertransferblotwasstainedwithmAb(2 µm/ml). FS:AntibodyMR5D3wasusedasaMRligandtotargetMR+dendtriticcellsresultinginanti-ratIgGproduction(Ref.6). |
| Use | Forimmunohistochemistry,flowcytometryandWesternblotting,dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.Forfunctionalstudies,dilutionshavetobeoptimizedinuser’sexperimentalsetting. |
| Immunogen | ChimaericCRD4-7-Fcprotein |
| Isotype | RatIgG2a |
| Species | Mouse |
| Alias | CD206,MMR,cLEC13D,Macrophagemannosereceptor1 |
| References | 1.Zamze,Setal;Recognitionofbacterialcapsularpolysaccharidesandlipopolysaccharidesbythemacrophagemannosereceptor.JBiolChem2002,277:41613 2.Martinez-Pomares,Letal.AnalysisofmannosereceptorregulationbyIL4,IL10anproteolyticprocessingusingnovelmonoclonalantibodies.J.ofLeukBiol2003,73:604 3.Hassan,Metal.Theschistosoamansonihepaticegggranulomaprovidesafavourablemicroenvironmentforsustainedgrowthofleishmaniadonovani.AmJPath2006,169:943 4.DUSPasquir,Metal.ThedermalmicroenvironmentinducestheexpressionofthealternativeactivationMarkerCD301/mGLinmononuclearphagocytes,independentofIL4/IL13signalling.JLeukBiol2006,80:838 5.Boskovic,Jetal.Structuralmodelforthemannosereceptorfamilyuncoveredbyelectronmicroscopyofendo180andthemannosereceptor.JBiolChem2006,281:8780 6.McKenzie,Eetal.Mannosereceptorexpressionandfunctiondefineanewpopulationofmurinedendriticcells.Jimmunol2007,178:4975 7.Miller,Jetal.Themannosereceptormediatesdenguevirusinfectionofmicrophages.Plospathogens2008,4:0001 8.Segura,Eetal.Differentcross-presentationpathwaysinsteady-stateandinflammatorydendriticcells.PNAS2009Dec1;106:20377 9.-Hardison,Setal.Interleukin-17isnotrequiredforclassicalmacrophageactivationinapulmonarymousemodelofCryptococcusneoformansinfection.Infect&immune2010,78:5341 10.-Geier,Hetal.Phagocyticreceptorsdictatephagosomalescapeandintracellularproliferationoffrancisellatularensis.InfectImmun2011,march21 |
| Storageandstability | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforatleastoneyear.Theexactexpirydateisindicatedonthelabel. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsibleforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
| Disease | Cardiologyandmetabolism,Infectiousdiseases |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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