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Hycult Biotech/TLR9, Mouse, mAb 5G5, biotinylated/HM1043/50 µg
Hycult Biotech/TLR9, Mouse, mAb 5G5, biotinylated/HM1043/50 µg
商品编号：
HM1043
品牌：
hycultbiotech
市场价：
¥5184.00
美元价：
3110.40
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:TLR9,Mouse,mAb5G5,biotinylated
Themonoclonalantibody5G5reactswiththeToll-likereceptor9(TLR9,CD289).TLRsarehighlyconservedthroughoutevolutionandhavebeenimplicatedintheinnatedefencetomanypathogens.InDrosophila,tollisrequiredfortheanti-fungalresponse,whiletherelated18-wheelerisinvolvedinantibacterialdefences.Inmammals,TLRsidentifiedastypeItransmembranesignallingreceptorswithpatternrecognitioncapABIlities,havebeenimplicatedintheinnatehostdefencetopathogens.AsinvestigatedsofarallfunctionalcharacterizedTLRsignalviatheTLR/IL-1receptor(IL-1R)pathwaywhererecruitmentofMyD88seemstobeessential.Incontrasttocell-wallcomponents,bacterialDNAisprobablyinvisIBLeforimmunecellsuntilDNAisliberatedduringprocessestakingplaceintheendosomal/lysosomalcompartmentwhereintracellularTLR9recruitsMyD88toinitiatesignaltransduction.UnmethylatedCpG-dinucleotide-containingsequencesarefoundmuchmorefrequentlyinbacterialgenomesthaninvertebratesgenomes,whereasthefrequencyofCpGdinucleotidesaresuppressedandusuallymethylated.TheregionsadjacenttotheCpGdinucleotidesalsoaffecttheimmunostimulatoryactivity.Theoptimalsequencedifferssignificantlybetweenmammalianspecies.MethylatedCpGdinucleotideslackimmunostimulatoryactivities.CellularactivationinresponsetobacterialDNAandsyntheticdinucleotidescontainingunmethylatedCpG-dinucleotidesismediatedbyTLR9.Themonoclonalantibody5G5reactswithRAWmacrophagesandTLR9transfectedHEK293cells,andiscrossreactivewithcanineTLR9.
Specifications:
| Catalognumber | HM1043 |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 50µg |
| Formulation | 0.5ml(100µg/ml)0.2µmfilteredbiotinylatedantibodysolutioninPBS,containing0.1%bovineserumalbuminand0.02%sodiumazide. |
| Conjugate | Biotin |
| Application | Immunoassays,Westernblot |
| ApplicationNotes | Themonoclonalantibody5G5canbeusedforflowcytometry,Westernblottingandimmunoassaysasdetectionantibody.FurThermore,themonoclonalantibody5G5isusefulforimmunohistologyonfrozenandparaffinsections.Themonoclonalantibody5G5stainsRAWmacrophagesandTLR9(CD289)transfectedHEK293cells.TheantibodyisweaklycrossreactivewithhumanTLR9. |
| Use | Forimmunohistology,flowcytometryandWesternblotting,dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
| Isotype | MouseIgG2a |
| Species | Mouse |
| Crossreactivity | Canine-Yes,Human-Yes |
| References | 1.Ahmad-Nejad,Petal;BacterialCpG-DNAandlipopolysaccharidesactivateToll-likereceptorsatdistinctcellularcompartments.EurJImmunol2002,32:1958 2.Rutz,Metal;Toll-likereceptor9bindssingle-strandedCpG-DNAinasequence-andpH-dependentmanner.EurJImmunol2004,34:2541 3.Burgener,Ietal;AntibodiesspecificforhumanormurineToll-likereceptorsdetectcanineleukocytesbyflowcytometry.VetImmunolImmunopathol2008,124;184 |
| Storageandstability | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsibleforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
| Disease | Infectiousdiseases |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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