4000-520-616
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当前位置: 首页 > 产品中心 > monoclonal_antibody > Hycult Biotech/TLR9,小鼠,单克隆抗体5G5,FITC/HM1042F/100µg
商品详细Hycult Biotech/TLR9,小鼠,单克隆抗体5G5,FITC/HM1042F/100µg
Hycult Biotech/TLR9,小鼠,单克隆抗体5G5,FITC/HM1042F/100µg
Hycult Biotech/TLR9,小鼠,单克隆抗体5G5,FITC/HM1042F/100µg
商品编号: HM1042F
品牌: hycultbiotech
市场价: ¥8736.00
美元价: 5241.60
产地: 美国(厂家直采)
公司:
产品分类: 单克隆抗体
公司分类: monoclonal_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Description:TLR9,Mouse,mAb5G5,FITC

Themonoclonalantibody5G5reactswiththeToll-likereceptor9(TLR9,CD289).TLRsarehighlyconservedthroughoutevolutionandhavebeenimplicatedintheinnatedefencetomanypathogens.InDrosophila,tollisrequiredfortheanti-fungalresponse,whiletherelated18-wheelerisinvolvedinantibacterialdefences.Inmammals,TLRsidentifiedastypeItransmembranesignallingreceptorswithpatternrecognitioncapABIlities,havebeenimplicatedintheinnatehostdefencetopathogens.AsinvestigatedsofarallfunctionalcharacterizedTLRsignalviatheTLR/IL-1receptor(IL-1R)pathwaywhererecruitmentofMyD88seemstobeessential.Incontrasttocell-wallcomponents,bacterialDNAisprobablyinvisIBLeforimmunecellsuntilDNAisliberatedduringprocessestakingplaceintheendosomal/lysosomalcompartmentwhereintracellularTLR9recruitsMyD88toinitiatesignaltransduction.UnmethylatedCpG-dinucleotide-containingsequencesarefoundmuchmorefrequentlyinbacterialgenomesthaninvertebratesgenomes,whereasthefrequencyofCpGdinucleotidesaresuppressedandusuallymethylated.TheregionsadjacenttotheCpGdinucleotidesalsoaffecttheimmunostimulatoryactivity.Theoptimalsequencedifferssignificantlybetweenmammalianspecies.MethylatedCpGdinucleotideslackimmunostimulatoryactivities.CellularactivationinresponsetobacterialDNAandsyntheticdinucleotidescontainingunmethylatedCpG-dinucleotidesismediatedbyTLR9.Themonoclonalantibody5G5reactswithRAWmacrophagesandTLR9transfectedHEK293cells,anditiscrossreactivewithcanineTLR9.

Specifications:

CatalognumberHM1042F
ProducttypeMonoclonalantibodies
Quantity100µg
Formulation1ml(100µg/ml)0.2µmfilteredFITCconjugatedantibodysolutioninPBS,containing1.0%bovineserumalbuminand0.02%sodiumazide.
ConjugateFITC
ApplicationFlowcytometry,Frozensections,Immunoassays,Paraffinsections,Westernblot
ApplicationNotesThemonoclonalantibody5G5canbeusedforflowcytometry,Westernblottingandimmunoassaysasdetectionantibody.FurThermore,themonoclonalantibody5G5isusefulforimmunohistologyonfrozenandparaffinsections.Themonoclonalantibody5G5stainsRAWmacrophagesandTLR9(CD289)transfectedHEK293cells.TheantibodyisweaklycrossreactivewithhumanTLR9.
UseForimmunohistology,flowcytometryandWesternblottingdilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.
IsotypeMouseIgG2a
SpeciesMouse
CrossreactivityCanine-Yes,Human-Yes
References1.Ahmad-Nejad,Petal;BacterialCpG-DNAandlipopolysaccharidesactivateToll-likereceptorsatdistinctcellularcompartments.EurJImmunol2002,32:1958
2.Rutz,Metal;Toll-likereceptor9bindssingle-strandedCpG-DNAinasequence-andpH-dependentmanner.EurJImmunol2004,34:2541
3.Burgener,Ietal;AntibodiesspecificforhumanormurineToll-likereceptorsdetectcanineleukocytesbyflowcytometry.VetImmunolImmunopathol2008,124;184
StorageandstabilityProductshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear.
PrecautionsForresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsibleforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct.
DiseaseInfectiousdiseases
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登,从1994开始制造检测抗体和蛋白质,主要侧重与病理学诊断有关的全套产品研发,既有纯化的重组蛋白和多肽,也有单抗和多抗,是世界一流的先天免疫领域的研究试剂制造商。