4000-520-616
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当前位置: 首页 > 产品中心 > monoclonal_antibody > Hycult生物技术/中性粒细胞,小鼠,单克隆抗体NIMP-R14,FITC/HM1039F/100µg
商品详细Hycult生物技术/中性粒细胞,小鼠,单克隆抗体NIMP-R14,FITC/HM1039F/100µg
Hycult生物技术/中性粒细胞,小鼠,单克隆抗体NIMP-R14,FITC/HM1039F/100µg
Hycult生物技术/中性粒细胞,小鼠,单克隆抗体NIMP-R14,FITC/HM1039F/100µg
商品编号: HM1039F
品牌: hycultbiotech
市场价: ¥7752.00
美元价: 4651.20
产地: 美国(厂家直采)
公司:
产品分类: 单克隆抗体
公司分类: monoclonal_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Description:Neutrophils,Mouse,mAbNIMP-R14,FITC

ThemonoclonalantibodyNIMP-R14ishighlyspecificformurineLy-6GandLy-6C.TheLy-6G/-6ClocusencodesafamilyofLy-6proteinsincludingLy-6GandLy-6C.Ly-6antigenshaveamolecularweightbetween15,000and18,000.Ly6GistogetherwithLy6cacomponentofthemyeloiddifferentiationantigenGr-1.Ly6GaGPI-anchoredproteinandisagoodMarkerofperipheralneutrophils.Althoughpredominantlypresentsonneutrophils,itisalsoexpressedonasubsetofeosinophils,differentiatingpre-monocytesandplasmacytoiddendriticcells.Ly6Cisamonocyte/macrophageandendothelialcelldifferentiationantigenregulatedbyinterferongamma,andmayplayaroleinthedevelopmentandmaturationoflymphocytes.Itisexpressedonbonemarrowcells,monocytes/macrophages,neutrophils,endothelialcells,andTcellsubsets.ExpressionofGr-1inbonemarrowcorrelateswithgranulocytedifferentiationandmaturation.However,thephysiologicalroleofLy6Galoneremainsstillunclear.ThemonoclonalantibodyNIMP-R14hasbeensuccessfullyusedtostainpolymorphonuclear(PMN)cellsandmonocytesforfluorescentactivatedcellsortingandinfrozenandparaffinsections.Treatmentwithantibodiesinvivoleadstoneutropeniaandhasinhibitoryeffectonlocalimmuneresponses.FurThermore,ithasbeenshowntobeusefulfordepletionofneutrophilsinmice.Itdepletesneutrophilsassoonas6hoursafterinjectionandupto6days.

Specifications:

CatalognumberHM1039F
ProducttypeMonoclonalantibodies
Quantity100µg
Formulation1ml(100μg/ml)0.2μmfilteredproteinGpurifiedFITCconjugatedantibodysolutioninPBS,containing1.0%BSAand0.02%sodiumazide.
ConjugateFITC
ApplicationFlowcytometry,Frozensections,Functionalstudies,Paraffinsections
ApplicationNotesIHC-F:Tissuewasfixedwithacetone(Ref6)
IHC-P:Blockingwith20%normalrabbitserum(Ref4)FC:5x105cellswereincubatedwith10μg/mlantibody(Ref3)
FS:Neutrophildepletion.MiceweretreatedwithNIMP-R14givenintraperitoneallyatadoseof1mg,6hbeforeinfection(Ref5)
UseDilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.
ImmunogenPurifiedBALB/cmouseneutrophils
IsotypeRatIgG2b
SpeciesMouse
AliasLymphocyteantigen6complexlocusproteinG6c,myeloiddifferentiationantigenGr1
Genename:Ly6g
PositiveControlMouseneutrophils
NegativeControlMousethymocytes
References1.Lopez,Aetal;Differentiationantigensonmouseeosinophilsandneutrophilsidentifiedbymonoclonalantibodies,BritJHaematology1984,57:489
2.McLaren,Detal;Schistosomamansoni:evidencethatimmunityinvaccinatedandchronicallyinfectedCBA/Camiceissensitivetotreatmentwithamonoclonalantibodythatdepletescutaneouseffectorcells.ParasiteImmunol1987,9:6673.VanLent,Petal;Monocytes/macrophagesratherthanPMNareinvolvedinearlycartilagedegradationincationicimmunecomplexarthrititsinmice.JLeukocBiol1997,61:267
4.Lubberts,Eetal;Adenoviralvector-mediatedoverexpressionofIL-4inthekneejointofmicewithcollagen-inducedarthritispreventscartilagedestruction.JImmunol1999,163:45465.Tacchini-Cottier,Fetal;AnimmunomodulatoryfunctionforneutrophilsduringtheinductionofaCD4+Th4responseinBALB/cmiceinfectedwithLeishmaniamajor.JImmunol2000,165:628
6.Vriesde,Betal;ComplementfactorC5amediatesrenalischemia-reperfusioninjuryindependentfromneutrophils.JImmunol2003,170:38837.Nagendra,Setal;Absenceofcross-reactivitybetweenmurineLy-6CandLy-6G.CytometryA2004,58:195
8.Navarini,Aetal;InnateImmune-induceddepletionofbonemarrowneutrophilsaggravatessystemicbacterialinfections.PNAS2009,106:71079.Paust,Hetal;Chemokinesplayacriticalroleinthecross-regulationofTh1andTh17immuneresponsesinmurinecrescenticglomerulonephritis.KidneyInternational,2012.
10.Gao,Netal;MatrixMetalloproteinase-13asaTargetforSuppressingCornealUlcerationCausedbyPseudomonasaeruginosaInfection.JInfectDis.2015212:116
StorageandstABIlityProductshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear.
PrecautionsForresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct.
DiseaseAutoimmunity,Infectiousdiseases
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登,从1994开始制造检测抗体和蛋白质,主要侧重与病理学诊断有关的全套产品研发,既有纯化的重组蛋白和多肽,也有单抗和多抗,是世界一流的先天免疫领域的研究试剂制造商。