Hycult Biotech/TLR4/MD-2，小鼠，单克隆抗体MTS510/HM1029/100µg
商品编号：
HM1029
品牌：
hycultbiotech
市场价：
¥6432.00
美元价：
3859.20
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:TLR4/MD-2,Mouse,mAbMTS510
ThemonoclonalantibodyMTS510reactswiththeToll-likereceptor4(TLR4,CD284)thatisassociatedwithMD2.TLRsareexpressedbyvariouscellsoftheimmunesystem,suchasmacrophagesanddendriticcells.TLRsareclassIreceptors,withasingleα-helixthatspansthecellmembrane.Theyrecognizeandrespondtomoleculesderivedfrombacterial,viralandfungalpathogens,suchaslipopolysaccharide(LPS)fromtheoutermembraneofGramnegativebacteria,peptidoglycanfragmentsfrombacterialcellwallsandsingle-strandedanddouble-strandedRNAfromviruses.Toll-likereceptor4(TLR4;CD284)hasbeenidentified,nexttoMD-2andCD14,asareceptorthatiscentraltotheinnateimmuneresponsetoLPSofGram-negativebacteria.TLR4isuniqueamongTLRsinitsABIlitytoactivatetwodistinctsignalingpathways;onepathwayisactivatedbytheadaptorsTIRAP(Toll/interleukin-1-receptor(TIR)-domain-containingadaptorprotein)andMyD88,whichleadstotheinductionofpro‑inflammatorycytokines.ThesecondpathwayisactivatedbytheadaptorsTRIF(TIR-domaincontainingadaptorproteininducinginterferon‑β)andTRAM(TRIFrelatedadaptormolecule),whichleadstotheinductionoftypeIinterferons.MD-2existsasacellsurfaceproteininassociationwithTLR4.ItalsoexistsassecretedformsconsistingofMD-2monomerandmultimers.CirculatingsMD-2ismainlypresentasadoubletof~20and25kD,representingdifferentiallyglycosylatedforms.UnlikeTLR4,sMD-2bindsdirectlyLPSwithouttheneedofsolubleCD14(sCD14).However,LPS-MD-2interactionsareincreasedwhenLPSispretreatedwithCD14.OnlymonomericsMD-2isBIOLOGicallyactiveandabletoassociatewithTLR4andLPS.sMD-2circulatesinplasmaofhealthyindividualsasanon-active,polymericprotein.Insepticplasma,thetotalamountofsMD-2wasstronglyelevatedandcontainedbothsMD-2polymersandmonomers.SolubleMD-2isproposedtobeanimportantmediatoroforganinflammationduringsepsis.Duringexperimentalhumanendotoxemia,themonomericandtotalsMD-2contentinplasmaincreasedwiththekineticsofanacutephaseprotein.ThisparallelsenhancedTLR4costimulatoryactivity.InvitrostudiesrevealedthatsMD-2releaseappearstoberestrictedtoendothelialanddendriticcells.ThemonoclonalantibodyMTS510reactspreferentially,especiallyinflowcytometry,withmouseTLR4thatisassociatedwithMD-2.MTS510isaTLR4function-blockingantibodythatisusefulforstudiesontheroleofTLR4asareceptorforLPSinducedcytokineproductionbyTLR4bearingcells.TheantibodywasshowntocoprecipitateMD-2(30kDa)withTLR4(100kDa).
Specifications:
| Catalognumber | HM1029 |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 100µg |
| Formulation | 1ml(100µg/ml)0.2µmfilteredantibodysolutioninPBSand0.1%bovineserumalbumin. |
| Application | Flowcytometry,Frozensections,Functionalstudies,Immunofluorescence,Immunoprecipitation |
| ApplicationNotes | F:6µmacetonefixedsectionsblockedwith0.3%hydrogenperoxidaseinmethanolandsubsequentlywithnormalserum.Sectionswereincubatedfor2hatRTat1/100dilutionofmAb Fc:Cellswereincubatedwith0.1 µmmAbfor30minutesat4 °C FS:Cellswerepre-incubatedwith10 µg/mlofantagoNISTicmAbMTS510 RAW264.7cells(105)werestainedwith4 µg/mlmAbfor1hat4 °C(black-isotypecontrol,red-irrelevantmAb,Blue-HM1029) |
| Use | Forimmunohistologyandflowcytometrydilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
| Immunogen | Ba/F3cellsexpressingmouseTLR4andMD-22 |
| Isotype | RatIgG2a |
| Species | Mouse |
| Alias | Toll-likereceptor4:-TLR4,CD284,ARMD10 MD2:ESOP-1;myeloiddifferentiationprotein-2;lymphocyteantigen96(Ly-96) |
| PositiveControl | RAW264.7cells |
| References | 1.Nomura,Fetal;Cuttingedge:Endotoxintoleranceinmouseperitonealmacrophagescorrelatewithdown-regulationofsurfacetoll-likereceptor4expression.JImmunol2000,164:3476 2.Akashi,Setal;Cuttingedge:Cellsurfaceexpressionandlipopolysaccharidesignallingviathetoll-likereceptor4-MD-2complexonmouseperitonealmacrophages.JImmunol2000,164:3471 3.Sato,Setal;Synergyandcross-tolerancebetweentoll-likereceptor(TLR)2-andTLR4-mediatedsignalingpathways.JImmunol2000,165:7096 4.Ortega-Cava,Cetal;Strategiccompartmentalizationoftoll-likereceptor4inthemousegut.JImmunol2003,170:3977 5.Tsujimoto,H.AcriticalroleofCpGmotifsinamurineperitonitismodelbytheirbindingtohighlyexpressedtoll-likereceptor-9onliverNTKcells.Jhepatology2006,45:836 6.Cook,Cetal.Lipopolysaccharide,TumorNecrosisFactoralpha,orInterleukin-1βtriggersreactivationoflatentCMVinimmunocompetentmice.Jvirology2006,80:9151 7.Tsukumo_Detal.Loss-of-functionmutationinToll-likereceptor4preventsdiet-inducedobesityandinsulinresistance.Diabetes2007,56:1986 8.Burton,Oetal.ImportanceofTLR2inthedirectresponseofTlymphocytestoSchistosomamansoniantigens.EurJImmunol2010,40:2221 |
| Storageandstability | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
| Disease | Infectiousdiseases |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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