Hycult Biotech/MCP-1，小鼠，单克隆抗体ECE.2/HM1019/100µg
商品编号：
HM1019
品牌：
hycultbiotech
市场价：
¥6192.00
美元价：
3715.20
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description: MCP-1, Mouse, mAb ECE.2
The monoclonal antibody ECE.2 recognizes mouse monocyte chemoattractant protein 1 (MCP-1).
The murine JE gene encodes the monocyte-specific cytokine monocyte chemotactic protein 1 (MCP-
1). MCP-1 is a CC chemokine of 76 amino acids (~11 kDa) and is chemotactic for monocytes and
basophils but not neutrophils and eosinophils. MCP-1 is expressed by smooth muscle cells (SMC),
macrophages, endothelial cells, keratinocytes and fibroblasts in response to inflammatory stimuli such
as interleukin 1β and tumor necrosis factor α. MCP-1 has been implicated in a variety of inflammatory
processes, including inflammatory bowel disease, rheumatoid arthritis, asthma, nephritis, and parasitic
and viral infections. MCP-1 antigen is not detected in the endothelium or SMC of normal arteries.
MCP-1 has also been shown to exhibit biological activities other than chemotaxis. It can induce the
proliferation and activation of killer cells known as CHAK (CC-Chemokine-activated killer)
MCP-1 signals via the CCR2 receptor, and is critical for aneurysm formation because of its stability to
recruit leukocytes. These leukocytes produce extracellular matrix-degrading MMPs, thereby inductin
aortic remodelling and dilatation. Interleukin-6 is also involved in this amplification loop accelerating
vascular inflammation. MCP-/- mice display significantly delayed wound re-epithelialization, and also
delayed wound angiogenesis.
Specifications:
| Catalog number | HM1019 |
|---|---|
| Product type | Monoclonal antibodies |
| Quantity | 100 µg |
| Formulation | 1 ml (100 μg/ml) 0.2 μm filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide. |
| Application | Frozen sections, Western blot |
| Application Notes | F: Sections (6 μm) were fixed with 4% PFA, blocked using 0.1% Triton-X and 5% serum for 15min at 37°C or o/n 4 °C and then incubated with 2 μg/ml antibody for 2h at 37°C. W: Samples electrophoresed on 15% SDS-PAGE were blotted on nitrocellulose and blocked with PBS/5% low fat dry milk. The blot was incubated with antibody (0.8 μg/ml) for 20 min at RT. |
| Use | Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. |
| Immunogen | Synthetic peptide corresponding to residues 102-130 of mouse MCP-1 |
| Isotype | Rat IgG1 |
| Species | Mouse |
| Cross reactivity | Human - No |
| Alias | C-C motif chemokine 2, Monocyte chemotactic and activating factor, Monocyte secretory protein JE, Small-inducible cytokine A2, Sigje Gene name: Ccl2 |
| References | 1. Zoja, C et al;- Renal expression of monocyte chemoattractant protein-1 in lupus autoimmune mice. J Am Soc Nephrol 1997, 8: 720 2. Kimura, Y et al; Facilitating action of asiaticoside at low doses on burn wound repair and its mechanism. Eur J Pharmacol 2008, 584: 415 3. Tieu, B et al. An adventitial IL-6/MCP1 amplification loop accelerates macrophage-mediated vascular inflammation leading to aortic dissection in mice. J Clin Invest 2009, 119: 3637 |
| Storage and stability | Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year. |
| Precautions | For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product. |
| Disease | Autoimmunity, Infectious diseases |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
联络我们
