Hycult生物技术/gC1q-R，人类，单克隆抗体74.5.2，功能研究抗体，低内毒素/HM2015-FS/0.5 mg
商品编号：
HM2015-FS
品牌：
hycultbiotech
市场价：
¥20832.00
美元价：
12499.20
产品分类：
标签抗体
公司分类：
Tag_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:gC1q-R,Human,mAb74.5.2,functionalstudyantibody,lowendotoxin
Themonoclonalantibody74.5.2recognizesacellmembraneC1qbindingmoleculethatrecognisestheglobularheadsofC1q.Itisalsopresentinplasmaandtheextracellularmatrix.Themoleculeisanunusuallyacidic,singlechainproteinwithanapparentmolecularweightof33kDa.ItdoesnotpossessaconventionalsequencemotifcompatIBLewithamembranespanningsegmentnoraconsensussiteforaGPIanchor.gC1q-Rmigratesasasinglechainunderbothreducingandnon-reducingconditions,butitbehavesasanoligomerongel-filtrationinnon-dissociatingconditions.ItsmultimerformationmaybeacriticalprocessbywhichthegC1q-RmoleculeincreasesitsaffinityformultivalentligandssuchasC1q.gC1q-RhasbeenshowntoinhibitcomplementactivationbypreventingthebindingofC1qtoantibodies,suggestingthatthebindingsiteforgC1q-Randthebindingsiteforimmunecomplexes,whicharepresentontheC1qglobular"heads",maybelocatedatthesameposition.gC1q-Riscapableofinteractingwithseveralproteinsinvolvedinbloodclotting,namely,thrombin,prothrombin,theheparinbindingformofvitronectin,theternarycomplex,vitronectin-thrombin-antithrombin,aswellashigh-molecular-weightkininogenandHagemanfactor.Besidesitsroleinthecomplementpathway,gC1q-RparticipatesinenhancementofFc-receptorandCR1-mediatedphagocytosis,procoagulantactivityonplatelets,andchemotacticactivityonmastcells,eosinophils,neutrophils,andfibroblasts.gC1q-Risexpressedonawidevarietyofcellsandcanserveasabindingsiteforplasmaandmicrobialproteins.ItsantigenicsitesmaybecrypticoncellsinsUSPensionbutbecomeexposedwhenthecellsarefixedbybifunctionalcross-linkers.Probablyitisalsoexpressedonthecellmembraneasatetramer.CrosslinkingoractivationmaythusbringaboutatetramericassemblyofgC1q-Rfollowedbyaconformationalchangewithinthemolecule,therebyexposingepitopeswhichareotherwisehidden.AformofGC1q-Risalsofoundinsidethecell.IntracellulargC1q-Rhasbeenshowntobindthecytoplasmictailofthe;1B-adrenergicreceptorandtoPKCµ.Themonoclonalantibody74.5.2isdirectedagainstepitopessituatedwithintheNH2-terminalstretchofgC1q-Rcorrespondingtoresidues76-93.Clone60.11recognizestheputativeC1qbindingsiteandreactswiththematureform,buthaspoorornoreactivitywiththetruncatedform,lackingresidues74-95.
Specifications:
| Catalognumber | HM2015-FS |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 0.5mg |
| Formulation | 0.5mg(100µg/ml)0.2µmfilteredantibodysolutioninPBS-(exactconcentrationisindicatedonthelabel) |
| Application | Flowcytometry,Functionalstudies,Immunoassays,Immunoprecipitation,Westernblot |
| Use | ForflowcytometryandWesternblotting,dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.Forfunctionalstudies,invitrodilutionshavetobeoptimizedinuser"sexperimentalsetting. |
| Immunogen | Recombinant-gC1q-RcorrespondingtomaturegC1q-R(aminoacids74-282) |
| Isotype | MouseIgG1 |
| Species | Human |
| Crossreactivity | Rat-Yes |
| Alias | gC1qreceptor,glycoproteingC1qBP,GC1q-Rprotein,hyaluronan-bindingprotein1,mitochondrialmatrixproteinp32,p33 |
| References | 1.Ghebrehiwet,Betal;IdentificationoffunctionaldomainsongC1Q-R,acellsurfaceproteinthatbindstotheglobular"heads"ofC1Q,usingmonoclonalantibodiesandsyntheticpeptides.Hybridoma1996,5:333 2.Ghebrehiwet,Betal;gC1q-R/p33,amemberofanewclassofmultifunctionalandmulticompartmentalcellularproteins,isinvolvedininflammationandinfection.ImmunolRev2001,180:65 3.Peerschke,Eetal;gC1qR/p33blockadereducesStaphylococcusaureuscolonizationoftargettissuesinananimalmodelofinfectiveendocarditis.InfectImmun2006,74:4418 4.Sansonno,Detal;RoleofthereceptorfortheglobulardomainofC1qproteininthepathogenesisofhepatitisCvirau-relatedcryoglobulinvasculardamage.JImmunol2009,183:6013 |
| StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsibleforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
| Disease | Cardiologyandmetabolism,Tumorimmunology |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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