小鼠功能性抗体，HyFs/R2，人功能性抗体，培养基为1055 mg/hmFs
商品编号：
HM1054-FS
品牌：
hycultbiotech
市场价：
¥20832.00
美元价：
12499.20
产品分类：
标签抗体
公司分类：
Tag_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:TLR2,Mouse/Human,mAbT2.5,functionalstudyantibody,lowendotoxin
ThemonoclonalantibodyT2.5recognizeshumanToll-likereceptor2(TLR2).Toll-likereceptors(TLR)arehighlyconservedthroughoutevolutionandhavebeenimplicatedintheinnatedefensetomanypathogens.Atpresent,ligandsforseveraloftheTLR"s,suchasTLR2-6,9,havebeenidentified,confirmingtheirroleinfirstlinedefenseagainstinvADIngmicroorganism.Inmammals,TLRsareidentifiedastypeItransmembranesignalingreceptorswithanextracellularportioncontainingleucine-richrepeatswithpatternrecognitioncapABIlities.PathogenrecognitionbyTLRsprovokesrapidactivationofinnateimmunitybyinducingproliferationofproinflammatorycytokinesandupregulationofcostimulatorymoleculesandeventuallytoinitiationofadaptiveimmunity.TLR2hasbeenidentifiedasareceptorthatiscentraltotheinnateimmuneresponsetolipoproteinsofGram-negativebacteria,severalwholeGram-positivebacteria,aswellasareceptorforpeptidoglycanandlipoteichoicacidandotherbacterialcellmembraneproducts.ItissuggestedthatTLR2isabletorecognizesuchawidevarietyofPAMPs(pathogen-specificmolecularpatterns)byformingheterodimerswithotherTLRslikee.g.TLR6..TLR2isessentialforrecognizinglipopeptidesandlipoproteinsfromseveralmicroorganismsandalsopeptidoglycansderivedfromgram-positivebacteria.Bacterialspeciesasdiverseasmycobacteria,spiRochetes,mycoplasma,Staphylococcusaureus,andStreptococcuspneumoniaehaveallbeenshowntomediatecellularactivationviaTLR2.
Specifications:
| Catalognumber | HM1054-FS |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 0.5mg |
| Formulation | 0.5mgof0.2μmfilteredproteinGpurifiedantibodysolutioninPBSwithaconcentrationofatleast0.5mg/ml(exactconcentrationisindicatedonthelabel).Theendotoxinconcentrationis<24EU/mg,determinedwithHIT302LALAssay. |
| Application | Flowcytometry,Frozensections,Functionalstudies,Immunoassays,Immunofluorescence,Immunoprecipitation |
| ApplicationNotes | IHC-F:6 µm;msectionswerefixedwithaceton.Sectionswereblockedwithgoatserumandexposedo/nwithT2.5. FC:4*104leukocytes/mlwerestainedfor30minutesat4 °C. FS:micewereinjectedi.p.with1mgT2.5,after1hincubationmicewerechallenged;T2.55 µg/mlwasaddedtocellculture. IP:40 µmclearedproteinwasincubatedwith2 µmT2.5for1hat4 °C. IA:T2.5asadetector. |
| Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.Forfunctionalstudies,invitrodilutionshavetobeoptimizedinuser’sexperimentalsetting. |
| Immunogen | MouseTLR2peptide |
| Isotype | MouseIgG1 |
| Species | Human/Mouse |
| Crossreactivity | Human-Yes |
| Alias | TLR2,CD282,TIL4 Genename:Tlr2 |
| PositiveControl | RAW264.7cells |
| NegativeControl | CHOcells |
| References | 1.Meng,Getal;AntagoNISTicantibodypreventstoll-likereceptor2-drivenlethalshock-likesyndromes.JClinInvest2004,113:1473 2.Roura-Mir,Cetal;MycobacteriumtuberculosisregulatesCD1antigenpresentationpathwaysthroughTLR-2.JImmunol2005,175:17583.Leemans,Jetal;Renal-associatedTLR2mediatesischemia/reperfusioninjuryinthekidney.JClinInvest2005115:2894 4.Spiller,Setal;TLR4-inducedIFN-γproductionincreasesTLR2sensitivityanddrivesGram-negativesepsisinmice.JEM2008,205:17475.Sutmuller,Retal;Toll-likereceptor2controlsexpansionandfunctionofregulatoryT-cells.JClinInvest2006,116:485 6.Brull,Fetal;TLR2activationisessentialtoinduceaTh1shiftinhumanperipheralbloodmononuclearcellsbyplantstanolsandplantsterols.JBiolChem2010,285:29517.Guan,Yetal;HumanTLRs10and1sharecommonmechanismofinnateimmunesensingbutnotsignaling.JImmunol2010,184:5094 8.Stribos,Eetal;RenalexpressionofToll-likereceptor2and4:Dynamicsinhumanallograftinjuryandcomparisontorodents.MolecularImmunology2015,64:829.Mason,Letal;BorreliaburgdorferiInducesTLR2-MediatedMigrationofActivatedDendriticCellsinanExVivoHumanSkinModel.PLoSONE2016,11:e0164040 10.Stoppelaar,Setal;Toll-LikeReceptorSignallingIsNotInvolvedinPlateletResponsetoStreptococcuspneumoniaeInVitroorInVivo.PLoSONE2016,11:e0156977 |
| Storageandstability | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
| Disease | Infectiousdiseases |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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