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4000-520-616
当前位置: 首页 > 产品中心 > Sandwich_method_ELISA > Hycult Biotech/Dx粪便钙卫蛋白IVD ELISA试剂盒/DH002/1 x 96检测。
商品详细Hycult Biotech/Dx粪便钙卫蛋白IVD ELISA试剂盒/DH002/1 x 96检测。
Hycult Biotech/Dx粪便钙卫蛋白IVD ELISA试剂盒/DH002/1 x 96检测。
Hycult Biotech/Dx粪便钙卫蛋白IVD ELISA试剂盒/DH002/1 x 96检测。
商品编号: DH002
品牌: hycultbiotech
市场价: ¥13944.00
美元价: 8366.40
产地: 美国(厂家直采)
公司:
产品分类: 夹心法ELISA
公司分类: Sandwich_method_ELISA
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Description:DxFecalCalprotectinIVDELISAkit

Calprotectin,alsoknownasS100A8/A9heterocomplex,isformedoutofthecalcium-binding,migratingproteinsS100A8andS100A9.Calprotectinisacalcium-andzinc-bindingproteincomplexcomposedof8and14kDsubunitsandcomplexformationiscalciumdependent.Neutrophilsarethemainproducersofcalprotectin,butexpressionhasalsobeenfoundinmonocytes,macrophagesandilealtissueeosinophils.Altogether,S100A8andS100A9proteinscontributetoapproximately40–60%ofthesoluble,cytosoliccontentofgranulocytes.Calprotectinhasantibacterial,antifungal,immunomodulatingandantiproliferativeeffects.Elevatedconcentrationsofcalprotectincanbemeasuredamongothersinplasma,synovialfluid,urineandfeces.Thepresenceofcalprotectininfecesquantitativelyrelatestoneutrophilmigrationtowardsthegastrointestinaltract.CalprotectinisthereforeconsideredareliablebioMarkerforintestinalinflammationthroughoutthegastrointestinaltract.Calprotectininfecesisstableforseveraldaysandevenlongerat4°C.ConsistentlyhigherfecalcalprotectinlevelshavebeenreportedinbothadultandpediatricpatientswithIBDcomparedtoIBSorhealthycontrols.Raisedlevelsoftheproteinindicatepresenceofluminalwhitecells.Suchfindingindicatesthattheintestineisinflamed,althoughthetypeofinflammationisnotdefined.Nowadays,itiscommonlythoughtthatconcentrationsof<50=""μg/g=""represent=""normal,=""50-150=""μg/g=""represent=""a=""weakly=""positive=""test=""and=""above=""these=""values=""indicate=""a=""positive=""test.=""however,=""these=""values=""are=""still=""under=""debate=""and=""might=""be=""laboratory=""dependent.=""in=""most=""cases,=""a=""negative=""calprotectin=""test=""rules=""out=""ibd,=""thereby=""sparing=""most=""people=""with=""ibs=""from=""having=""an=""endoscopic=""procedure.=""the=""elisa=""is=""a=""single=""incubation=""step=""reaction.=""after=""this=""incubation=""the=""wells=""are=""filled=""with=""substrate.=""the=""obtained=""color=""intensity=""is=""proportional=""to=""the=""amount=""of=""calprotectin=""in=""your=""sample.=""the=""amount=""of=""calprotectin=""can=""be=""calculated=""based=""on=""the=""standard=""curve=""of=""calibrated=""native=""calprotectin.=""the=""test=""performance=""is=""assured=""by=""the=""addition=""of=""a=""high=""control=""and=""a=""low=""control.="">

Specifications:

CatalognumberDH002
ProducttypeAssays
Quantity1x96det.
StandardrangeMeasurableconcentrationrangeof16-625μg/g(RangeI;50xsampledilution)or48-1,875μg/g(RangeII;150xsampledilution).
DetectionlevelTheminimumconcentrationwhichcanbemeasuredis16μg/g.
Workingvolume100µl/well
SpeciesHuman
AliasS100A8/S100A9,MRP8/MRP14
ApplicationTheDxFecalCalprotectinassayisasandwichELISAspecificallydevelopedforquickandreliableinvitroquantificationoffecalcalprotectininstoolsamples.Thisquantificationenablestodiscriminatebetweeninflammatoryboweldisease(IBD)andirritablebowelsyndrome(IBS)andalsoallowsmonitoringofdiseasestateoractivityinIBDpatients.Theresultsofthekitaretobeusedincombinationwithotherlaboratoryandclinicalfindingsforthepurposeofprovidinginformationaboutapathologicalstate.Theanalysisshouldbeperformedbytrainedlaboratoryprofessionals.
DiseaseGastroenterology
PrincipleTheDxFecalCalprotectinELISAisaready-to-usesolid-phaseenzyme-linkedimmunoassaywithaworkingtimeof1hourand30minutes.Theefficientformatof1platewithtwelvedisposable8-wellstripsallowsfreechoiceofbatchsizefortheassay.BiotinylatedcaptureantibodyandHRPconjugateddetectionantibodywillbindtocalprotectinpresentinstandardandsamplesinthemixture.Biotinylatedcaptureantibodywillbindtothestreptavidincoatedplates,incomplexwiththestandardorsamplesandtheHRPconjugateddetectionantibody.HRPconjugateddetectionantibodywillreactwiththesubstrate,tetramethylbenzidine(TMB).Theenzymereactionisstoppedbytheadditionofoxalicacid.Theabsorbanceat450nmismeasuredwithaspectrophotometer.Astandardcurveisobtainedbyplottingtheabsorbance(linear)versusthecorrespondingconcentrationsofthecalprotectinstandards(log).Thecalprotectinconcentrationofsamplesandcontrols,whicharerunconcurrentlywiththestandards,canbedeterminedfromthestandardcurve.
RecoveryRecoverywasdeterminedbymixingfecalextractionsampleswithaknowncalprotectinconcentrationindifferentratios.Therecoveryrangedbetween84.9%and116.2%.
AutomatedELISAperformanceTheassaycanbeperformedonanautomatedELISAplatformwhichiscapabletoshaketheplateduringincubation.Pleasenotethatshakingthedevicebelowtherecommendedspeedof500rpmwillresultinalowerabsorbance.AssaywastestedonaDynexDS2toevaluatetestperformance.Programforthisplatformisavailableonrequest.
PrecisionandReproducibilityTheintra-assayprecisionandreproducibilitywastestedwithextractsoffourfecalsamplesusingtwentyidenticalaliquotsincludingthehighcontrolandlowcontrol.TheCVrangedbetween3.9and5.5%.Todeterminetheinter-assayvariation,fivesamplesweretestedbytwooperatorsbothmanuallyandautomatically(DynexDS2)onthreeseparatedays.TheCVinthissituationranged2.8and9.2%
StorageandstABIlityProductshouldbestoredat4°C.Underrecommendedstorageconditions,productisstableforoneyear.
References1.Sipponen,Tetal;Fecalcalprotectinindiagnosisandclinicalassessmentofinflammatoryboweldisease.ScandJGastroenterol2015,50:1
2.Henderson,Petal;ThediagnosticaccuracyoffecalcalprotectinduringtheinvestigationofsUSPectedpediatricinflammatoryboweldisease:asystematicreviewandmetaanalysis.AmJGastroenterol2014,109:5
3.Aggio,Retal;Futuremethodsforthediagnosisofinflammatoryboweldisease.DigDis2014,32:4
4.Fengming,Yetal;Biomarkersofinflammatoryboweldisease.DisMarkers2014
5.Stragier,Eetal;TheuseoffecalcalprotectinandlactoferrininpatientswithIBD.Review.ActaGastroenterolBelg2013,76:3
6.Montalto,Metal;Roleoffecalcalprotectiningastrointestinaldisorders.EurRevMedPharmacolSci2013,17:12
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登,从1994开始制造检测抗体和蛋白质,主要侧重与病理学诊断有关的全套产品研发,既有纯化的重组蛋白和多肽,也有单抗和多抗,是世界一流的先天免疫领域的研究试剂制造商。