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当前位置: 首页 > 产品中心 > Sandwich_method_ELISA > Hycult Biotech/RNase 7, Human, ELISA kit/HK371-01/1 x 96 det.
商品详细Hycult Biotech/RNase 7, Human, ELISA kit/HK371-01/1 x 96 det.
Hycult Biotech/RNase 7, Human, ELISA kit/HK371-01/1 x 96 det.
Hycult Biotech/RNase 7, Human, ELISA kit/HK371-01/1 x 96 det.
商品编号: HK371-01
品牌: hycultbiotech
市场价: ¥12660.00
美元价: 7596.00
产地: 美国(厂家直采)
公司:
产品分类: 夹心法ELISA
公司分类: Sandwich_method_ELISA
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Description:RNase7,Human,ELISAkit

Antimicrobialproteins(AMP)areafundamentalelementoftheprimaryresponseagainstpathogens.AMPsaresmallendogenouscationicmoleculesexpressedbyphagocyticandepithelialcells.TheantimicrobialactivityofAMPsisdirectedtowardsabroadspectrumofpathogens,likeGram-positive&negativebacteria,viruses,yeastandfungi.AMPsaidininnateandadaptiveimmunityviadirectinactivationandbyimmunomodulatoryactivitylikeleukocytemigration.RNase7isanproteinsecretedbyavarietyofepithelialtissuesandisamemberoftheRNaseAsuperfamily.Thisfamilysharessequenceandstructuralsimilaritiessuchasconservedcysteineresiduesaswellasconservedhistidinesandalysineintheactivecentercatalysingtheribonucleaseactivity.RNase7isa14.5KDaproteinwithdistinctribonucleaseactivity.However,theantimicrobialactivityisindependentformtheribonucleaseactivitywhichmightbeassociatedwithantiviralactivity.Onapermolarbasis,RNase7isoneofthemostpotentAMPs.RNase7contributestothesterilityinseveralsystems.Itisdescribedtobeimportantinsterilityofthekidneyandurinarytractaswellasitscontributiontotheskinbarrierprotection.Intheurinarysystemitisconstitutivelyexpressedbytheintercalatedcellsintherenalcollectingtubulesandispresentintheurineatsuchlevelstokillbacteriaatbaseline.Intheskinitcontributestocontrolthegrowthofmicroorganismsontheskinsurface,andtheexpressionlevelscanbefurtherinducedundercontrolofproinflammatorycytokines.ThebactericidalactivityofRNase7hasbeenassociatedwithitsABIlitytobindandpermeatethebacterialcellmembrane.Thisrequiresclusteringoflysineresidues.RNase7isabletobindLPSandpeptidoglycans.Inocularsurface,signaltransductionassociatedwithRNase7expressionismediatedviaMAPKsbutnotNF-κBsignallingpathways.IL1βleadstoanincreasedexpressionofRNase7.

Specifications:

CatalognumberHK371-01
ProducttypeAssays
Quantity1x96det.
Standardrange78.1to5000pg/ml
Detectionlevel78.1pg/ml
Workingvolume100µl/well
SpeciesHuman
AliasRNase7,Ribonuclease7,Skin-derivedantimicrobialprotein2
ApplicationThehumanRNase7ELISAkitistobeusedfortheinvitroquantitativedeterminationofhumanRNase7inserum,plasma,bronchoalveolarlavagefluid,urineandcellculturesupernatantsamples.
DiseaseInfectiousdiseases,Nephrology
PrincipleThehumanRNase7ELISAisaready-to-usesolid-phaseenzyme-linkedimmunosorbentassaybasedonthesandwichprinciplewithaworkingtimeof3½hours.Theefficientformatofaplatewithtwelvedisposable8-wellstripsallowsfreechoiceofbatchsizefortheassay.SamplesandstandardsareincubatedinmicrotiterwellscoatedwithantibodiesrecognizinghumanRNase7.BiotinylatedtracerantibodywillbindtothecapturedhumanRNase7.Streptavidin-peroxidaseconjugatewillbindtothebiotinylatedtracerantibody.Streptavidin-peroxidaseconjugatewillreactwiththesubstrate,tetramethylbenzidine(TMB).Theenzymereactionisstoppedbytheadditionofoxalicacid.Theabsorbanceat450nmismeasuredwithaspectrophotometer.Astandardcurveisobtainedbyplottingtheabsorbance(linear)versusthecorrespondingconcentrationsofthehumanRNase7standards(log).ThehumanRNase7concentrationofsamples,whicharerunconcurrentlywiththestandards,canbedeterminedfromthestandardcurve.
StorageandstabilityProductshouldbestoredat4°C.Underrecommendedstorageconditions,productisstableforoneyear.
PrecautionsForresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct.
References1.KötenB,SimanskiM,GläserR,PodschunR,SchröderJ-M,etal.(2009)RNase7ContributestotheCutaneousDefenseagainstEnterococcusfaecium.PLoSONE4(7):e6424
2.SchwadererA,WangH,BartzJ,KlineJ,EichlerT,DeSouzaK,Sims-LucasS,BakerP,D(2013)Ribonuclease7,anantimicrobialpeptideup-regulatedduringinfection,contributestomicrobialdefenseofthehumanurinarytract.KidneyInt.83(4):615–625.3.BecknellB,EichlerT,BeceiroS,LiB,EasterlingR,CarpenterA,JamesC,McHughK,HainsD,Partida-SanchezS,SpencerJ.(2014)Ribonucleases6and7haveantimicrobialfunctioninthehumanandmurineurinarytract.KidneyInt.2014Jul30
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登,从1994开始制造检测抗体和蛋白质,主要侧重与病理学诊断有关的全套产品研发,既有纯化的重组蛋白和多肽,也有单抗和多抗,是世界一流的先天免疫领域的研究试剂制造商。