Hycult Biotech/TNF-α，人，ELISA试剂盒/HK307-01/1 x 96检测。
商品编号：
HK307-01
品牌：
hycultbiotech
市场价：
¥7992.00
美元价：
4795.20
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:TNF-alpha,Human,ELISAkit
Tumornecrosisfactoralpha(TNF-alpha),ahomotrimeric17KDprotein,isapotentmediatorofinflammatoryandmetabolicfunctions.TNF-alphawasoriginallydetectedasahighlycytotoxiccytokinefortumorcells,itcausestumornecrosisinvivoandshowscytolyticactivityagainsttumorcellsinvitro.FurThermore,TNF-alphahasbeenimpliedascentralmediatorinshockinducedbygramnegativemicro-organisms.ThecytokineTNFisfoundtobeacentralmediatorinmanyinflammatoryandimmunologicalprocesses:itcanbeinducedbyvariousproductsofmicro-organismsandbyvariouscytokinesbutitalsoinducesonitsturntheproductionofmanycytokines.SignaltransductionoccursviatwotypesofTNF-receptors,thep55(RI)andp75(RII)TNF-receptors.Thereceptorsdifferstronglyintheirintra-cellularsignalingpathways.TheTNF-alphatrimerinteractswitheitherofthetwotypesofTNF-RleADIngtoreceptorcross-linking.
Specifications:
| Catalognumber | HK307-01 |
|---|---|
| Producttype | Assays |
| Quantity | 1x96det. |
| Standardrange | 8-2,000pg/ml |
| Detectionlevel | 10pg/ml |
| Workingvolume | 100µl/well |
| Species | Human |
| Application | ThehumanTNF-αELISAkitistobeusedfortheinvitroquantitativedeterminationofhumanTNF-αinserum,plasma,andcellculturesupernatantsamples. |
| Disease | Autoimmunity,Gastroenterology |
| Principle | ThehumanTNF-alphaELISAisaready-to-usesolid-phaseenzyme-linkedimmunosorbentassaybasedonthesandwichprinciplewithaworkingtimeof3½hours.Theefficientformatofaplatewithtwelvedisposable8-wellstripsallowsfreechoiceofbatchsizefortheassay.Samplesandstandardsarecapturedbyasolidboundspecificantibody.BiotinylatedtracerantibodywillbindtocapturedhumanTNF-alpha.Streptavidin-peroxidaseconjugatewillbindtothebiotinylatedtracerantibody.Streptavidin-peroxidaseconjugatewillreactwiththesubstrate,tetramethylbenzidine(TMB).Theenzymereactionisstoppedbytheadditionofcitricacid.Theabsorbanceat450nmismeasuredwithaspectrophotometer.Astandardcurveisobtainedbyplottingtheabsorbance(linear)versusthecorrespondingconcentrationsofthehumanTNF-alphastandards(log).ThehumanTNF-alphaconcentrationofsamples,whicharerunconcurrentlywiththestandards,canbedeterminedfromthestandardcurve. |
| StorageandstABIlity | Productshouldbestoredat4°C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
| References | 1.Molnar,Letal;DetectionofTNFalphaexpressioninthebonemarrowanddeterminationofTNFalphaproductionofperipheralbloodmononuclearcellsinmyelodysplasticsyndrome.PatholOncolRes2000,6:18 2.Younis,Aetal;Staphylococcusaureusleucocidin,avirulencefactorinbovinemastitis.JDairyResearch2005,72:188 |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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