Hycult Biotech/MASP-3,人,ELISA试剂盒/HK339-02/2 x 96检测。
商品编号:
HK339-02
品牌:
hycultbiotech
市场价:
¥23664.00
美元价:
14198.40
产品分类:
夹心法ELISA
公司分类:
Sandwich_method_ELISA
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Description:MASP-3,Human,ELISAkit
Themannan-bindinglectin(MBL)pathwayofthecomplementsystemisinitiatedbythebindingofMBLorficolintospecificcarbohydratestructuresfoundonthesurfaceofavarietyofmicroorganisms.ThisbindingisfollowedbytheactivationofMBL-associatedserineproteases(MASPs).TherearefourknownMASPs:MASP-1,MASP-2,MASP-3andMap19(alsoknownassMAP,asmall,separatelysynthesizedfragmentofMASP-2).MASPsaresecretedassinglechainprecursorsandareprocessedintoaheavychain(A)andcatalyticlightchain(B)linkedbydisulfidebonds.AllMASPsformhomo-dimersandindividuallyassociatewithMBLandtheficolinsinaCa2+-dependentmanner.MASP-3isanalternativelysplicedproductfromtheMASP-1gene,containinganidenticalAchain,butanentirelydifferentBchain.MASP-3isanapproximately100kDaproteinandassociateswithbothMBLandL-ficolininthepresenceofCa2+.TheMASP3dimercanbedisruptedbyEDTA.MASP-3isexpressedmainlybutnotexclusivelyintheliver.LiverexpressedMASP-3issecretedintotheblood.UnlikeMASP-2,noproteolyticactivityoncomplementproteinssC2,C3andC4forMASP-3hasbeendetected.MASP-3exertsinhibitoryactivityonMASP-2function.However,nonaturalsubstratesforMASP-3areknown.MASP-3concentrationinhealthyhumanserumisnormallydistributedwithameanvalueof3796ng/ml.MASP-3ispresentatbirthatapproximately80%ofthelevelmeasured6,9and12monthsafterbirth.TheMASP-3levelisstablethroughouta1-yearperiod.ThehighexpressionofMASP-3inastrocytesindicatesthatMASP-3mightalsobeinvolvedinotherfunctionsthatcouldbeimportantinthebrain.Inmultiplesclerosispatients,highMASP-3levelsseemtohaveapositiveeffect(medianMASP-3levels:4539ng/ml).MASP-3deficienciesarecurrentlynotreported.
Specifications:
Catalognumber | HK339-02 |
---|---|
Producttype | Assays |
Quantity | 2x96det. |
Standardrange | 8–500ng/ml |
Detectionlevel | 8ng/ml |
Workingvolume | 100µl/well |
Species | Human |
Application | ThehumanMASP-3ELISAkitistobeusedfortheinvitroquantitativedeterminationofbothnaturalandrecombinanthumanMASP-3inserumandplasmasamples. |
Disease | Autoimmunity,Infectiousdiseases |
Principle | TheMASP-3ELISAisaready-to-usesolid-phaseenzyme-linkedimmunosorbentassaybasedonthesandwichprinciplewithaworkingtimeof4½hours.Theefficientformatof2plateswithtwelvedisposable8-wellstripsallowsfreechoiceofbatchsizefortheassay.Samplesandstandardsarecapturedbyasolidboundspecificantibody.BiotinylatedtracerantibodywillbindtocapturedMASP-3.Streptavidin-peroxidaseconjugatewillbindtothebiotinylatedtracerantibody.Streptavidin-peroxidaseconjugatewillreactwiththesubstrate,tetramethylbenzidine(TMB).Theenzymereactionisstoppedbytheadditionofoxalicacid.Theabsorbanceat450nmismeasuredwithaspectrophotometer.Astandardcurveisobtainedbyplottingtheabsorbance(linear)versusthecorrespondingconcentrationsoftheMASP-3standards(log).TheMASP-3concentrationofsamples,whicharerunconcurrentlywiththestandards,canbedeterminedfromthestandardcurve. |
Linearity | Thelinearityoftheassaywasdeterminedbyseriallydilutingasamplecontaining250ng/mlhumanMASP-3.Thedilutedsamplesweremeasuredintheassay.Thelineobtainedaslopeof0.970andacorrelationcoefficientof0.999. |
Recovery | Normalhumanbloodsamples,containingbaselinelevelsofMASP-3,werespikedwithrecombinantMASP-3inconcentrationsof100and10ng/ml.SampleswithandwithoutMASP-3wereincubatedfor30minutesat37°C.SamplesweremeasuredusingtheELISA.ValuesforMASP-3rangedbetween93%and116%(mean110%). |
StorageandstABIlity | Productshouldbestoredat4°C.Underrecommendedstorageconditions,productisstableforoneyear. |
Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
References | 1.Frauenknecht,Vetal;Plasmalevelsofmannan-bindinglectin(MBL)-associatedserineproteases(MASPs)andMBL-associatedproteinincardio-andcerebrovasculardiseases.Clinical&ExperimentalImmunology2013 |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登,从1994开始制造检测抗体和蛋白质,主要侧重与病理学诊断有关的全套产品研发,既有纯化的重组蛋白和多肽,也有单抗和多抗,是世界一流的先天免疫领域的研究试剂制造商。
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