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Hycult Biotech/Arginase I, Human, ELISA kit/HK322-02/2 x 96 det.
Hycult Biotech/Arginase I, Human, ELISA kit/HK322-02/2 x 96 det.
商品编号：
HK322-02
品牌：
hycultbiotech
市场价：
¥25728.00
美元价：
15436.80
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:ArginaseI,Human,ELISAkit
Arginase-I(liver-typearginase,L-arginineureahydrolase,orL-arginineamidinohydrolase)isanhydrolyticenzymethatconvertsargininetoornithineandurea.Ornithineistheprecursorforpreline,anessentialaminoacidforcollagensynthesisandforpoly-amines,keycomponentsincellgrowthanddifferentiation.
Arginaseexistsastwoformsdesignatedarginase-Iandarginase-IIwhichareencodedbydifferentgenes.Althougharginase-Iandarginase-IIhavesimilarenzymeactivities,theydifferinpIandimmunologicalreactivity.Humanarginase-I(liver-typearginase)isa35kDproteincirculatinginbloodprobablyasahomotrimer.Liver-typearginaseismostabundantexpressedinmammalianliver,butisalsofoundinnon-hepatictissues,forinstanceredbloodcells,lactatingmammalianglands,andthekidney.Inadditiontoitsinvolvementinammoniadetoxificationviatheureacycle,arginaseplaysaroleinotherprocesses,forinstancemacrophage-mediatedcytotoxicityduetoarginasereleaseandinhibitionoflymphocyteproliferationinvitro.Itshowshighactivityingrowingtissues,woundhealing,proliferatinglymphocytesandtumors.FurThermore,arginaseactsasamodulatoroftheimmuneresponse.Besidesthisarginaseplaysaroleinallergenchallengedlungs,inautoimmuneinflammationinthecentralnervoussystemandinacuteliverinjury.
Inplasmaofhealthyindividualsarginase-Iispresentinlevelsof1.8-30ng/ml,whichincreasesapproximately10foldduringacutephaseresponses.
Arginaseexistsastwoformsdesignatedarginase-Iandarginase-IIwhichareencodedbydifferentgenes.Althougharginase-Iandarginase-IIhavesimilarenzymeactivities,theydifferinpIandimmunologicalreactivity.Humanarginase-I(liver-typearginase)isa35kDproteincirculatinginbloodprobablyasahomotrimer.Liver-typearginaseismostabundantexpressedinmammalianliver,butisalsofoundinnon-hepatictissues,forinstanceredbloodcells,lactatingmammalianglands,andthekidney.Inadditiontoitsinvolvementinammoniadetoxificationviatheureacycle,arginaseplaysaroleinotherprocesses,forinstancemacrophage-mediatedcytotoxicityduetoarginasereleaseandinhibitionoflymphocyteproliferationinvitro.Itshowshighactivityingrowingtissues,woundhealing,proliferatinglymphocytesandtumors.FurThermore,arginaseactsasamodulatoroftheimmuneresponse.Besidesthisarginaseplaysaroleinallergenchallengedlungs,inautoimmuneinflammationinthecentralnervoussystemandinacuteliverinjury.
Inplasmaofhealthyindividualsarginase-Iispresentinlevelsof1.8-30ng/ml,whichincreasesapproximately10foldduringacutephaseresponses.
Specifications:
| Catalognumber | HK322-02 |
|---|---|
| Producttype | Assays |
| Quantity | 2x96det. |
| Standardrange | 0.4-100ng/ml |
| Detectionlevel | 2.6ng/ml |
| Workingvolume | 100µl/well |
| Species | Human |
| Application | ThehumanArginase-IELISAkitistobeusedfortheinvitroquantitativedeterminationofnaturalandrecombinanthumanArginase-Iinserum,plasmaandcellculturesupernatantsamples. |
| Disease | Autoimmunity,Tumorimmunology |
| Principle | Thehumanarginase-IELISAisaready-to-usesolid-phaseenzyme-linkedimmunosorbentassaybasedonthesandwichprinciplewithaworkingtimeof3½hours.Theefficientformatofaplatewithtwelvedisposable8-wellstripsallowsfreechoiceofbatchsizefortheassay.Samplesandstandardsarecapturedbyasolidboundspecificantibody.Biotinylatedtracerantibodywillbindtocapturedhumanarginase-I.Streptavidin-peroxidaseconjugatewillbindtothebiotinylatedtracerantibody.Streptavidin-peroxidaseconjugatewillreactwiththesubstrate,tetramethylbenzidine(TMB).Theenzymereactionisstoppedbytheadditionofoxalicacid.Theabsorbanceat450nmismeasuredwithaspectrophotometer.Astandardcurveisobtainedbyplottingtheabsorbance(linear)versusthecorrespondingconcentrationsofthearginase-Istandards(log).Thehumanarginase-Iconcentrationofsamples,whicharerunconcurrentlywiththestandards,canbedeterminedfromthestandardcurve. |
| Linearity | Thelinearityoftheassaywasdeterminedbyseriallydilutingasamplecontaining335ng/mlhumanarginase-I.Thedilutedsamplesweremeasuredintheassay.Thelineobtainedaslopeof1.044andacorrelationcoefficientof0.995. |
| StorageandstABIlity | Productshouldbestoredat4°C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
| References | 1.Ikemoto,Metal;AusefulELISAsystemforhumanliver-typeArginase,anditsutilityindiagnosisofliverdiseases.ClinBiochem2001,34:455 2.Debats,Ietal;Infectedchronicwoundsshowdifferentlocalandsystemicarginineconversioncomparedwithacutewounds.JSurgRes2006,134:205 3.Weber,Wetal;Asyntheticmetabolite-basedmammalianinter-cellsignallingsystem.MolBioSyst2009 |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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