Hycult生物技术/PECAM-1，小鼠，单克隆抗体MEC7.46，生物素化/HM1014/50µg
商品编号：
HM1014
品牌：
hycultbiotech
市场价：
¥4392.00
美元价：
2635.20
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:PECAM-1,Mouse,mAbMEC7.46,biotinylated
ThemonoclonalantibodyMEC7.46recognizesthemouseformoftheplatelet-endothelialcelladhesionmolecule(PECAM)-1(CD31).PECAM-1isamemberoftheimmunoglobulinsuperfamily.Thisheavilyglycosylatedproteinisfoundintheentirevascularendotheliumofadultmiceandfunctionsinmediatingcellularadhesionbyheterophilicandhomophilicmechanisms.PECAM-1isdetectedwithinthelymphopoieticislandsinthespleenofnewborn(day12)andinthebonemarrowofadultmice.CapillaryendothelialcellsofadultmicealsoexpressPECAM-1.
ThereactivityofthemonoclonalantibodyMEC7.46isrestrictedtotheisoformofthemoleculethatisselectivelyexpressedbyendothelialcells.Theantibodyprecipitatesa130kDamoleculepresentonthemembraneofendothelialcellsofallmousebloodvesselsbothinnormal,inflamedandtumortissues.TheantigenispredominantlypresentatthelateralbordersofendothelialcellsasdescribedforhumanPECAM-1.StainingofMEC7.46canbeseenoncapillaries,veins,arteriesandliversinusoids.
ThereactivityofthemonoclonalantibodyMEC7.46isrestrictedtotheisoformofthemoleculethatisselectivelyexpressedbyendothelialcells.Theantibodyprecipitatesa130kDamoleculepresentonthemembraneofendothelialcellsofallmousebloodvesselsbothinnormal,inflamedandtumortissues.TheantigenispredominantlypresentatthelateralbordersofendothelialcellsasdescribedforhumanPECAM-1.StainingofMEC7.46canbeseenoncapillaries,veins,arteriesandliversinusoids.
Specifications:
| Catalognumber | HM1014 |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 50µg |
| Formulation | 0.5ml(100μg/ml)0.2μmfilteredproteinGpurifiedbiotinylatedantibodysolutioninPBS,containing0.1%bovineserumalbuminand0.02%sodiumazide. |
| Conjugate | Biotin |
| Application | Flowcytometry,Frozensections,Immunofluorescence,Immunoprecipitation,Paraffinsections |
| ApplicationNotes | FC:AntibodyMEC7.46stainstheextracellulardomainofmousePECAM-1;106cellswereusedpersample.NofixationorpermeABIlizationneeded.Epitopeissensitivetotrypsin!(Ref.1) F:Tissuesectionswerefixedinacetonefor10minatRTandpretreatedwith0.01%hydrogenperoxidetoquenchendogenousperoxidases.Normalrabbitserumwasusedasblockingagent.As(Ref.1).P:Serialparaffin-embedded4μmsectionswerestainedbyH&E,PASandAzanHistochemistry.Thedistributionofendothelial(Mec7.46),matrix(laminin)andmacrophage(FA/11)Markerswasevaluatedusingastandardthree-stepABCmethodanddevelopmentin3-amino-9-ethylcarbazolesolution.Sectionswerecounterstainedfor45secondswithHarris"haematoxylinatroomtemperatureandmountedinImsol-mountmedium(ref.3). IP:Lysateof35S-Methioninelabeled5HVcellswasused.Moleculeof~130kDa. |
| Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
| Immunogen | Mouset.end.1cells(polyomamiddleT(PmT)-transformedEC) |
| Isotype | RatIgG1 |
| Species | Mouse |
| Alias | CD31,platelet-endothelialcelladhesionmolecule-1 Genename:Pecam1 |
| PositiveControl | Mouset-end.1cells |
| NegativeControl | L929cells(Fibroblasts) |
| References | 1.Vecchi,Aetal;Monoclonalantibodiesspecificforendothelialcellsofmousebloodvessels.Theirapplicationintheidentificationofadultandembryonicendothelium.EurJCellBiol1994,63:247 2.Kusters,Betal.VascularEndothelialGrowthFactor-A165InducesProgressionofMelanomaBrainMetastaseswithoutInductionofSproutingAngiogenesis.CancerRes2002,62:3413.Clarijs,Retal;Lymphaticsandlymphatic-likestructuresinmelanoma.Thesis2003 4.Xu,Qetal;CirculatingProgenitorcellsregenerateendotheliumofveingraftatherosclerosis,whichisdiminishedinApoE-deficientmice.CircRes2003,93:e765.Smetsers,Tetal;Localizationandcharacterizationofmelanoma-associatedglycosaminoglycans:Differentialexpressionofchondroitinandheparansulfateepitopesinmelanoma.CancRes2003,63:2965 |
| Storageandstability | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
| Disease | Cardiologyandmetabolism |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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