Hycult Biotech/CD34, Mouse, mAb MEC14.7/HM1015/100 µg
商品编号：
HM1015
品牌：
hycultbiotech
市场价：
¥5424.00
美元价：
3254.40
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:CD34,Mouse,mAbMEC14.7
ThemonoclonalantibodyMEC14.7recognizesmouseCD34,asingle-passtypeImembraneglycophosphoproteinpresentonsmallvesselendothelialcellsandhematopoieticProgenitorcells.TheapparentmolecularmassofCD34isheterogeneous,dependingontheglycosylationstateindifferentcelltypes.Inculturedendotheliomacelllysate,CD34hasamolecularweightof~100kDa,whereasinlunglysatesitis~80kDa.2IsoformsofCD34exist,bothareexpressedonthecellsurface.CD34isanadhesionmoleculeperformingaroleinearlyhematopoiesisbymediatingtheattachmentofstemcellstothebonemarrowextracellularmatrixordirectlytostromalcells.CD34actsasascaffoldfortheattachmentoflineagespecificglycans,allowingstemcellstobindtolectinsexpressedbystromalcellsorothermarrowcomponents.CD34presentscarbohydrateligandstoselectins.CD34iswidelyusedasaMarkertoselectearlyhematopoieticstemandprogenitorcellsinexperimentalandclinicalhematopoiesis.ThemonoclonalantibodyMEC14.7recognizesaneuraminidasesensitiveepitopeonendotheliuminvivo,particularlyonsmallvesselsandneoformedcapillariesanddevelopingvascularstructuresinembryonalstructures.ThemonoclonalantibodyMEC14.7canbeusedforidentificationandcharacterizationofcapillaryendothelialcells.FurThermore,theantibodyisusefulforisolationandcharacterizationofhematopoieticprogenitorcells,particularlyofmyelomonocyticcolonyformingcells.MonoclonalantibodyMEC14.7isalsousefulforimmunopurificationandcellseparation
Specifications:
| Catalognumber | HM1015 |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 100µg |
| Formulation | 1ml(100µg/ml)0.2µmfilteredantibodysolutioninPBS,containing0.1%bovineserumalbuminand0.02%sodiumazide. |
| Application | Flowcytometry,Frozensections,Immunofluorescence,Immunoprecipitation,Paraffinsections,Westernblot |
| ApplicationNotes | W:bothreduced/non-reduced;70-105kDa,dependingonglycosylationstateandcelltype(Ref1) P:Formalinfixed;boiledtwicefor5minutesincitrate(pH6.0)asantigenretrieval(inhousetested;Ref4,6)or0.03%trypsintreatmentfor10minutesat37 °C(Ref3) FC:micebloodcells;0.1 µgMEC14.7/106cells(Ref5) IP:~100kDaproteininH5Vcells(Ref1)CD34inmouseglioma.StainingofparaffintissuesectionwithantibodyMEC14.7(Cat.#HM1015).Anti-mouseCD34at5 µg/ml(o/n,4 °C)resultedinthespecificstainingofendothelialcells. |
| Use | Forimmunohistology,flowcytometryandWesternblotting,dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.Forfunctionalstudies,invitrodilutionshavetobeoptimizedinuser"sexperimentalsetting. |
| Immunogen | MurinetransformedECt-end.1 |
| Isotype | RatIgG2a |
| Species | Mouse |
| Alias | HematopoieticprogenitorcellantigenCD34 |
| PositiveControl | H5Vcells |
| References | 1.Garlanda,Cetal;CharacterizationofMEC14.7,anewmonoclonalantibodyrecognizingmouseCD34,ausefulreagentforidentifyingandcharacterizingbloodvesselsandhematopoieticprecursors.EurJCellBiol1997,73:368 2.Dong,Qetal;Ageneralstrategyforisolationofendothelialcellsfrommurinetissues:Characterizationoftwoendothelialcelllinesfromthemurinelungandsubcutaneousspongeimplants.ArteriosclerThrombVascBiol1997,17:1599 3.Solberg,Hetal;AfunctionaloverlapofplasminogenandMMPsregulatesvascularizationduringplacentaldevelopment.Development2003,130:4439 4.Almholt,Ketal;Metastasisoftransgenicbreastcancerinplasminogenactivatorinhibitor-1gene-deficientmice.Oncogene2003,22:4389 5.Sho,Eetal;HemodynamicregulationofCD34+celllocalizationanddifferentiationinexperimentalaneurysms.ArteriosclerThrombVascBiol2004,24:1916 6.Suganuma,Tetal;FunctionalexpressionoftheangiotensinIItype1receptorinhumanovariancarcinomacellsanditsblockadetherapyresultinginsuppressionoftumorinvasion,angiogenesis,andperitonealdissemination.ClinCancerRes2005,11:2686 |
| StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
| Disease | Tumorimmunology |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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