Hycult Biotech/C3b/iC3b/C3c，小鼠，单克隆抗体2/11，免疫分析抗体/HM1065-IA/0.5mg
商品编号：
HM1065-IA
品牌：
hycultbiotech
市场价：
¥26232.00
美元价：
15739.20
产品分类：
标签抗体
公司分类：
Tag_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:C3b/iC3b/C3c,Mouse,mAb2/11,immunoassayantibody
Themonocolonalantibody2/11recognizesactivatedfragmentsofthemousecomplementproteinC3.Thecomplementsystemisanimportantpartofthehumoralresponseininnateimmunity,consistingofthreedifferentpathways.Thethirdcomplementcomponent,C3,iscentraltotheclassical,alternativeandlectinpathwaysofcomplementactivation.Activationproductsofthecomplementcascadecontainneo-epitopesthatarenotpresentintheindividualnativecomponents.
ThesynthesisofC3istissue-specificandismodulatedinresponsetoavarietyofstimulatoryagents.C3isthemostabundantproteinofthecomplementsystemwithserumproteinlevelsofabout1.3mg/ml.AninheriteddeficiencyofC3predisposesthepersontofrequentassaultsofbacterialinfections.Inulcerativecolitis,andidiopathicchronicinflammatoryboweldisease,thedepositionofC3inthediseasedmucosahasbeenreported.
ProteolysisbycertainenzymesresultsinthecleavageofC3intoC3aandC3b.C3bbecomesattachedtoimmunecomplexesandisfurthercleavedintoiC3b,C3c,C3dgandC3f.Themonoclonalantibody2/11isspecificforcleavedC3fragmentsC3b,iC3b,andC3c,andforactivatedC3.Therefore,positivereactivityintissuesisassociatedwithactivationofhecomplementcascadeandC3cleavage.IncaseofanacuteinflammatoryreactionlotsofC3areprocessedintotheproductsrecognizedby2/11andisassuchusefulasMarkerforinflammatoryreaction.Inchronicinflammatoryconditionsminimalreactivitywith2/11mayobserved.InsuchcasesprimarilytheC3dgproductresidesattheplaceofinflammation(C3cbeingcleared)whichisnotrecognizedbyantibody2/11.Thechronicprocessing/activationofC3istakingplaceatalowerlevel,whichwouldreducedetectionoftheC3fragmentsC3b,iC3b,andC3c.
NexttodetectionofC3fragmentsC3b,iC3b,andC3c,themonoclonalantibody2/11inhibitsthehemolyticactivityofmousecomplementinadose-dependentmanner.
ThesynthesisofC3istissue-specificandismodulatedinresponsetoavarietyofstimulatoryagents.C3isthemostabundantproteinofthecomplementsystemwithserumproteinlevelsofabout1.3mg/ml.AninheriteddeficiencyofC3predisposesthepersontofrequentassaultsofbacterialinfections.Inulcerativecolitis,andidiopathicchronicinflammatoryboweldisease,thedepositionofC3inthediseasedmucosahasbeenreported.
ProteolysisbycertainenzymesresultsinthecleavageofC3intoC3aandC3b.C3bbecomesattachedtoimmunecomplexesandisfurthercleavedintoiC3b,C3c,C3dgandC3f.Themonoclonalantibody2/11isspecificforcleavedC3fragmentsC3b,iC3b,andC3c,andforactivatedC3.Therefore,positivereactivityintissuesisassociatedwithactivationofhecomplementcascadeandC3cleavage.IncaseofanacuteinflammatoryreactionlotsofC3areprocessedintotheproductsrecognizedby2/11andisassuchusefulasMarkerforinflammatoryreaction.Inchronicinflammatoryconditionsminimalreactivitywith2/11mayobserved.InsuchcasesprimarilytheC3dgproductresidesattheplaceofinflammation(C3cbeingcleared)whichisnotrecognizedbyantibody2/11.Thechronicprocessing/activationofC3istakingplaceatalowerlevel,whichwouldreducedetectionoftheC3fragmentsC3b,iC3b,andC3c.
NexttodetectionofC3fragmentsC3b,iC3b,andC3c,themonoclonalantibody2/11inhibitsthehemolyticactivityofmousecomplementinadose-dependentmanner.
Specifications:
| Catalognumber | HM1065-IA |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 0.5mg |
| Formulation | 0.5mgof0.2μmfilteredproteinGpurifiedantibodysolutioninPBSwithaconcentrationofatleast0.5mg/ml(exactconcentrationisindicatedonthelabel). |
| Application | Flowcytometry,Frozensections,Functionalstudies,Immunoassays,Immunofluorescence,Westernblot |
| ApplicationNotes | F:Fixationofsectionsinacetonefor5‘,pretreatmentwith3%hydrogenperoxideinmethanolatRTtoquenchendogenousperoxidases.Asnegativecontrol,sectionsfromC3-deficientmicewereused.(Ref.1) FC:Mousesplenocyteswereincubatedwithautologous,freshlydrawnmouseserumtofixtheC3fragmentsbeforestaining.AsnegativecontrolsplenocytesofC3deficientmicewasused.Antibody2/11stainstheneoantigenicsiteontheC3activationfragments.(Ref.1) FS:Antibody2/11inhibitshemolysisinadose-dependentmanner.(Ref.1) W:Anon-reducedsampletreatmentandSDS-PAGEwasused.Thebandsizeis150kDa(Ref.1). IA:Supernatantofantibody2/11wasincubatedonanti-ratIgGFccoatedplates.Afterincubationwithsample,HRP-conjugatedpolyclonalgoatanti-mouseC3wasusedfordetection.(Ref.1) |
| Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
| Immunogen | MouseC3 |
| Isotype | RatIgG1 |
| Species | Mouse |
| PositiveControl | Mousesplenocytespreincubatedwithserumfromthesameanimal. |
| NegativeControl | C3-deficientmousecells. |
| References | 1.Mastellos,Detal;NovelmonoclonalantibodiesagainstmouseC3interferingwithcomplementactivation:descriptionoffinespecificityandapplicationstovariousimmunoassays.MolImmunol2004,40:1213 2.Markiewski,Metal;C3aandC3bactivationproductsofthethirdcomponentofcomplement(C3)arecriticalfornormalliverrecoveryaftertoxicinjury.JImmunol2004,173:747 3.Markiewski,Metal;Modulationoftheantitumorimmuneresponsebycomplement.NatImmunol2008,9:1225 |
| StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
| Disease | Infectiousdiseases,Nephrology |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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