Hycult Biotech/E-selectin，人，单克隆抗体ENA1，免疫分析抗体/HM4001-IA/0.5mg
商品编号：
HM4001-IA
品牌：
hycultbiotech
市场价：
¥19640.00
美元价：
11784.00
产品分类：
标签抗体
公司分类：
Tag_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:E-selectin,Human,mAbENA1,immunoassayantibody
ENA1reactswithE-selectinCD62-E,previousdesignatedtheEndothelialLeucocyteAdhesionMolecule-1(ELAM-1).TheantibodyreactswithhumanendothelialcellsactivatedwithTNF-alpha,IL-1orendotoxin.TheantibodywasfoundtoreactalsowithcellstransfectedwiththeE-selectingene.Theantibodyinhibitstheadhesionofgranulocytesbothneutrophilicandeosinophilic.
Specifications:
| Catalognumber | HM4001-IA |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 0.5mg |
| Formulation | 0.5mgof0.2μmfilteredantibodysolutioninPBS(exactconcentrationisindicatedonthelabel). |
| Application | Frozensections,Functionalstudies,Immunoassays,Immunoprecipitation |
| Use | Forimmunohistologydilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:10.Invitroculturedcellscanbefixedwith1%paraformaldehydeandkeptinPBSplusazidebeforestaining.Tissuesectionsareadvisedtobefixedfor10mininpureacetoneandfollowedbyincubationfor10mininchloroform.Incubationwithapretesteddilutionoftheantibodyisadvisedtobefollowedbyabiotinconjugatedanti-murineIgandafurtherincubationwithanenzyme(alkalinephosphatase)conjugatedstreptavidin.Forselectionofthemostusefuldilutioninagivensituationateststainingwithcellsortissueknowntoexpresstheantigenshouldbeperformed.Tothisendeitherculturedendothelialcellsorasmallfreshskinbiopsycanbeincubatedfor4hourswithTNF-alpha(1ng/ml),IL-1(100U/ml)orLPS(1µg/ml)intissueculturemediumat37°C.AsnegativecontrolitisadvisedtouseacontrolmurineIgGantibody. |
| Isotype | MouseIgG1 |
| Species | Human |
| Crossreactivity | Baboon-Yes,Chimpanzee-Yes,Cynomolgusmonkey-Yes,Rhesusmonkey-Yes |
| References | 1.Leeuwenberg,JFMetal;Inductionofanactivationantigenonhumanendothelialcellsinvitro.EurJImmunol1989,19:715 2.Leeuwenberg,JFMetal;Adhesionofpolymorphonuclearcellstohumanendothelialcells.Adhesionmoleculedependent,andFc-receptor-mediatedadhesion-molecule-independentmechanisms.ClinandExpImmunol1990,81:496 3.Leeuwenberg,JFMetal;FunctionalpolymorphismofIgGFcRII(CD32)onhumanneutrophils.Immunology1990,71:301 4.Leeuwenberg,JFMetal;Interferon-gammaregulatestheexpressionofanadhesionmoleculeELAM-1andIL-6productionbyhumanendothelialcellsinvitro.JImmunology1990,145:2110 |
| StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
| Disease | Cardiologyandmetabolism,Neurologicaldisorders |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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