人功能性抗体，HyFs/20mg甘露糖受体，低分子量甘露糖研究
商品编号：
HM2056-FS
品牌：
hycultbiotech
市场价：
¥26232.00
美元价：
15739.20
产品分类：
标签抗体
公司分类：
Tag_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:Mannosereceptor,Human,mAb15-2,functionalstudyantibody,lowendotoxin
Themonoclonalantibody15-2recognizesthemannosereceptor(MR),alsoknownasCD206,amemberofthevertebrateC-typelectinfamily.Themannosereceptor,isapatternrecognitionreceptorthatisinvolvedinbothinnateandadaptiveimmunity.The175kDasingle-passtypeItransmembranereceptorconsistsof5domains:anamino-terminalcysteine-richregion,afibronectintypeIIrepeat,aseriesofeighttandemlectin-likecarbohydraterecognitiondomains(responsIBLefortherecognitionofmannoseandfucose),atransmembranedomain,andanintracellularcarboxy-terminaltail.
Thestructureissharedbythefamilyofmultilectinmannosereceptors:thephospholipaseA2-receptor,DEC205andthenovelC-typelectinreceptor(mannosereceptorX).TheMRbindshigh-mannosestructureson-awiderangeofgrampositiveandgramnegativebacteria,yeasts,parasitesandmycobacteria.TheMRhasalsobeenshowntobindandinternalizetissue-typeplasminogenactivator.
MR"sarepresentonmonocytesanddendriticcells(DC)andarepresumedtoplayaroleininnateandadaptiveimmunity,thelatterviaprocessingbyDC.TheexpressionofMRasobservedinimmunohistologyispresentontissuemacrophages,dendriticcells,asubpopulationofendothelialcells,Kupffercellsandspermcells.TheexpressionofMRonmonocytesincreasesduringcultureandcanbeenhancedbycytokinesasIFN-gamma.LabelingofMRexpressingmonocytes/macrophagesincreaseswithprolongedincubationtimeprobablyduetointernalizationoftheMR-antibody-complex.Themonoclonalantibody15-2preventsbindingofglycoproteinsincludingt-PAtoMR.
DetectionoftheMRwithanti-MRmonoclonalantibody15-2cansubstitutestainingformannosecontainingprobesaslabeledmannosylatedBSA,atechniquewhichismorecumbersomeandlessspecific.
Thestructureissharedbythefamilyofmultilectinmannosereceptors:thephospholipaseA2-receptor,DEC205andthenovelC-typelectinreceptor(mannosereceptorX).TheMRbindshigh-mannosestructureson-awiderangeofgrampositiveandgramnegativebacteria,yeasts,parasitesandmycobacteria.TheMRhasalsobeenshowntobindandinternalizetissue-typeplasminogenactivator.
MR"sarepresentonmonocytesanddendriticcells(DC)andarepresumedtoplayaroleininnateandadaptiveimmunity,thelatterviaprocessingbyDC.TheexpressionofMRasobservedinimmunohistologyispresentontissuemacrophages,dendriticcells,asubpopulationofendothelialcells,Kupffercellsandspermcells.TheexpressionofMRonmonocytesincreasesduringcultureandcanbeenhancedbycytokinesasIFN-gamma.LabelingofMRexpressingmonocytes/macrophagesincreaseswithprolongedincubationtimeprobablyduetointernalizationoftheMR-antibody-complex.Themonoclonalantibody15-2preventsbindingofglycoproteinsincludingt-PAtoMR.
DetectionoftheMRwithanti-MRmonoclonalantibody15-2cansubstitutestainingformannosecontainingprobesaslabeledmannosylatedBSA,atechniquewhichismorecumbersomeandlessspecific.
Specifications:
| Catalognumber | HM2056-FS |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 0.5mg |
| Formulation | 0.5mgof0.2μmfilteredproteinGpurifiedbiotinylatedantibodysolutioninPBSwithaconcentrationofatleast0.5mg/ml(exactconcentrationisindicatedonthelabel).Theendotoxinconcentrationis<24EU/mg,determinedwithHIT302LALAssay. |
| Application | Flowcytometry,Frozensections,Functionalstudies,Westernblot |
| ApplicationNotes | F:Tissuesectionswerefixedinacetone/chloroform.Sectionswerepreincubatedwithnormalrabbitserum(Ref.3). FC:Antibody15-2stainstheextracellulardomainofMR.W:Anon-reducedsampletreatmentand6%SDS-Pagewasused.Thebandsizeis175kDa(Ref.1). FS:Antibody15-2functionsasaninhibitorofbinding.Theantibodywasfunctionallytestedbypreincubationofimmobilizedreceptorwithantibody.Thispreventedbindingoft-PAtoimmobilizedreceptor.(Ref.1). |
| Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
| Immunogen | Purifiedhumanmannosereceptorfromhumanplacentaltissue |
| Isotype | MouseIgG1 |
| Species | Human |
| Alias | MachrophageMannoseReceptor1,MRC1,CLEC13D,MMR,C-typelectindomainfamily13memberD Genename:MRC1 |
| PositiveControl | Macrophages(incoronaryatheroscleroticplaque),placentatissue,Hofbauercells(fetalmacrophages),livertissue |
| NegativeControl | Lymphocytes,Monocytes |
| References | 1.Barret-Berghoeff,Metal;Monoclonalantibodiesagainstthehumanmannosereceptorthatinhibitthebindingoftissue-typeplasminogenactivator.ThrombHaemostas1997,77:718 2.Noorman,Fetal;MonoclonalantibodiesagainstthehumanmannosereceptorasaspecificMarkerinflowcytometryandimmunohistochemistryformacrophages.JLeukocyteBiol1997,61:633.Noorman,Fetal;Clustermannosidescaninhibitmannosereceptor-mediatedtissue-typeplasminogenactivatordegradationbybothratandhumancells.Hepatology1997,26:1303 4.Tailleux,Letal;DC-SIGNisthemajormycobacteriumtuberculosisreceptoronhumandendriticcells.JExpMed2003,197:1215.Pressice,Petal;Keyholelimpethemocyanininducestheactivationandmaturationofhumandendriticcellsthroughtheinvolvementofmannosereceptor.MolImmunol2008,45:1136 6.Tahara,Netal;2-deoxy-2-[(18)F]fluoro-d-mannosepositronemissiontomographyimaginginatherosclerosis.NatMed2014,20:215 |
| StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsibleforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
| Disease | Cardiologyandmetabolism,Infectiousdiseases |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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