Hycult生物技术/中性粒细胞，小鼠，单克隆抗体NIMP-R14，功能研究抗体，低内毒素/HM1039-FS/0.5 mg
商品编号：
HM1039-FS
品牌：
hycultbiotech
市场价：
¥20832.00
美元价：
12499.20
产品分类：
标签抗体
公司分类：
Tag_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:Neutrophils,Mouse,mAbNIMP-R14,functionalstudyantibody,lowendotoxin
ThemonoclonalantibodyNIMP-R14ishighlyspecificformurineLy-6GandLy-6C.TheLy-6G/-6ClocusencodesafamilyofLy-6proteinsincludingLy-6GandLy-6C.Ly-6antigenshaveamolecularweightbetween15,000and18,000.Ly6GistogetherwithLy6cacomponentofthemyeloiddifferentiationantigenGr-1.Ly6GaGPI-anchoredproteinandisagoodMarkerofperipheralneutrophils.Althoughpredominantlypresentsonneutrophils,itisalsoexpressedonasubsetofeosinophils,differentiatingpre-monocytesandplasmacytoiddendriticcells.Ly6Cisamonocyte/macrophageandendothelialcelldifferentiationantigenregulatedbyinterferongamma,andmayplayaroleinthedevelopmentandmaturationoflymphocytes.Itisexpressedonbonemarrowcells,monocytes/macrophages,neutrophils,endothelialcells,andTcellsubsets.ExpressionofGr-1inbonemarrowcorrelateswithgranulocytedifferentiationandmaturation.However,thephysiologicalroleofLy6Galoneremainsstillunclear.ThemonoclonalantibodyNIMP-R14hasbeensuccessfullyusedtostainpolymorphonuclear(PMN)cellsandmonocytesforfluorescentactivatedcellsortingandinfrozenandparaffinsections.Treatmentwithantibodiesinvivoleadstoneutropeniaandhasinhibitoryeffectonlocalimmuneresponses.FurThermore,ithasbeenshowntobeusefulfordepletionofneutrophilsinmice.Itdepletesneutrophilsassoonas6hoursafterinjectionandupto6days.
Specifications:
| Catalognumber | HM1039-FS |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 0.5mg |
| Formulation | 0.5mgof0.2μmfilteredproteinGpurifiedantibodysolutioninPBSwithaconcentrationofatleast0.5mg/ml(exactconcentrationisindicatedonthelabel).Theendotoxinconcentrationis<24EU/mg,determinedwithHIT302LALAssay. |
| Application | Flowcytometry,Frozensections,Functionalstudies,Immunoassays,Paraffinsections |
| ApplicationNotes | IHC-F:Tissuewasfixedwithacetone(Ref6) IHC-P:Blockingwith20%normalrabbitserum(Ref4)FC:5x105cellswereincubatedwith10μg/mlantibody(Ref3) FS:Neutrophildepletion.MiceweretreatedwithNIMP-R14givenintraperitoneallyatadoseof1mg,6hbeforeinfection(Ref5) |
| Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
| Immunogen | PurifiedBALB/cmouseneutrophils |
| Isotype | RatIgG2b |
| Species | Mouse |
| Alias | Lymphocyteantigen6complexlocusproteinG6c,myeloiddifferentiationantigenGr1 Genename:Ly6g |
| PositiveControl | Mouseneutrophils |
| NegativeControl | Mousethymocytes |
| References | 1.Lopez,Aetal;Differentiationantigensonmouseeosinophilsandneutrophilsidentifiedbymonoclonalantibodies,BritJHaematology1984,57:489 2.McLaren,Detal;Schistosomamansoni:evidencethatimmunityinvaccinatedandchronicallyinfectedCBA/Camiceissensitivetotreatmentwithamonoclonalantibodythatdepletescutaneouseffectorcells.ParasiteImmunol1987,9:6673.VanLent,Petal;Monocytes/macrophagesratherthanPMNareinvolvedinearlycartilagedegradationincationicimmunecomplexarthrititsinmice.JLeukocBiol1997,61:267 4.Lubberts,Eetal;Adenoviralvector-mediatedoverexpressionofIL-4inthekneejointofmicewithcollagen-inducedarthritispreventscartilagedestruction.JImmunol1999,163:45465.Tacchini-Cottier,Fetal;AnimmunomodulatoryfunctionforneutrophilsduringtheinductionofaCD4+Th4responseinBALB/cmiceinfectedwithLeishmaniamajor.JImmunol2000,165:628 6.Vriesde,Betal;ComplementfactorC5amediatesrenalischemia-reperfusioninjuryindependentfromneutrophils.JImmunol2003,170:38837.Nagendra,Setal;Absenceofcross-reactivitybetweenmurineLy-6CandLy-6G.CytometryA2004,58:195 8.Navarini,Aetal;InnateImmune-induceddepletionofbonemarrowneutrophilsaggravatessystemicbacterialinfections.PNAS2009,106:71079.Paust,Hetal;Chemokinesplayacriticalroleinthecross-regulationofTh1andTh17immuneresponsesinmurinecrescenticglomerulonephritis.KidneyInternational,2012. 10.Gao,Netal;MatrixMetalloproteinase-13asaTargetforSuppressingCornealUlcerationCausedbyPseudomonasaeruginosaInfection.JInfectDis.2015212:116 |
| StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
| Disease | Autoimmunity,Infectiousdiseases |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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