Hycult生物技术/脂多糖核心，单克隆抗体WN1 222-5，功能研究抗体，低内毒素/HM6011-FS/0.5 mg
商品编号：
HM6011-FS
品牌：
hycultbiotech
市场价：
¥18020.00
美元价：
10812.00
产品分类：
标签抗体
公司分类：
Tag_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:LipopolysaccharideCore,mAbWN1222-5,functionalstudyantibody,lowendotoxin
ThemousemonoclonalantibodycloneWN1222-5recognizesthecoreregionoflipopolysaccharide(LPS),whereasitlacksreactivitywithfreelipidAandRd2orsmallerLPS.LPSisaconstituentoftheoutermembraneofthecellwallofcertaintypesofGram-negativebacteria,suchasE.coli,Salmonella,Shigella,Pseudomonas,Neisseria,Haemophilus,andsomeotherlesserknownpathogens.TheendotoxinsofGram-negativebacteriaareLPSmoleculeswiththreedistinctdomainsreferredtoaslipidA,coreoligosaccharideandO-polysaccharide.ThelipidAandcoreoligosaccharidecomprisetheendotoxincoreandarerelativelyconservedamongdifferentGram-negativebacterialspecies.TheO-polysaccharidesshowwidestructuraldiversityandgiverisetotheO-specificityofdifferentstrainandspeciesSEROtypes.LipidAisthetoxicmoietyofendotoxin,andiscovalentlylinkedtocoreoligosaccharideinallLPS.Rough(R)bacterialacktheO-polysaccharideoftheirsmooth(S)strainbacteriacounterparts,anddifferentroughmutantbacteriahavebeenisolatedexpressingarangeofincompletecoreoligosaccharidestructuresrangingfromcompletecore(Ra)todeep-rough(Re)mutantsexpressingonlylipidAlinkedtoinner-coreKDOresidues.
Duringlife,humansaswellasothervertebrates,areoftenexposedtoLPS,forinstancebyenterobacteria.Whilegrowing,enterobacteriareleasessmallamountsofendotoxins.Predominantpartoftheendotoxinsstayonthecellwalluntilthebacteriumdisintegrates.Endotoxinsareheatstable,soevenboilingtheinfectedmaterialfor30minuteswillnotdenatureit.WaterydiarrheaiscausedbyreleasedLPSthatinteractswiththedigestivetrackintestine.CellsandtissuecanbedestroyedbytheLPSresultingininflammation.FurThermore,LPSisacausalfactorofNEC(necrotizingenterocolitis),adisorderfoundmostlyinnewborninfants.IfLPSgainsentrytothebloodstream,itcanbindthehostcells,suchasmacrophages.LPSresponsesarecomplicatedandinvolvecomplexesofLPS-bindingprotein(LBP),solubleand/ormembraneboundCD14andTolllikereceptor4(TLR4).LBPisaserumproteinthatcatalyzesLPSrecognitionbyCD14.RecognitionofLPStriggersacascadeofadversesystemicresponsesandorganfailure(septicshock).
Duringlife,humansaswellasothervertebrates,areoftenexposedtoLPS,forinstancebyenterobacteria.Whilegrowing,enterobacteriareleasessmallamountsofendotoxins.Predominantpartoftheendotoxinsstayonthecellwalluntilthebacteriumdisintegrates.Endotoxinsareheatstable,soevenboilingtheinfectedmaterialfor30minuteswillnotdenatureit.WaterydiarrheaiscausedbyreleasedLPSthatinteractswiththedigestivetrackintestine.CellsandtissuecanbedestroyedbytheLPSresultingininflammation.FurThermore,LPSisacausalfactorofNEC(necrotizingenterocolitis),adisorderfoundmostlyinnewborninfants.IfLPSgainsentrytothebloodstream,itcanbindthehostcells,suchasmacrophages.LPSresponsesarecomplicatedandinvolvecomplexesofLPS-bindingprotein(LBP),solubleand/ormembraneboundCD14andTolllikereceptor4(TLR4).LBPisaserumproteinthatcatalyzesLPSrecognitionbyCD14.RecognitionofLPStriggersacascadeofadversesystemicresponsesandorganfailure(septicshock).
Specifications:
| Catalognumber | HM6011-FS |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 0.5mg |
| Formulation | 0.5mgof0.2μmfilteredproteinGpurifiedantibodysolutioninPBSwithaconcentrationofatleast0.5mg/ml(exactconcentrationisindicatedonthelabel).Theendotoxinconcentrationis<24EU/mg,determinedwithHIT302LALAssay. |
| Application | Functionalstudies,Immunoassays,Paraffinsections,Westernblot |
| ApplicationNotes | W:Anon-reducedsampletreatmentandSDS-PAGEwasused(Ref.1and5). IHC-P:Antigenretrievalwasperformedbyheatingsectionsin1xDIVADecloakerreagent.AspositivecontrolgutorlymphnodeofSIV-infectedrhesusmacaqueswasusedandasnegativecontrolgutorlymphnodeofnon-SIV-infectedrhesusmacaques(Ref.6). |
| Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
| Immunogen | 10E8heat-killedbacteria |
| Isotype | MouseIgG2a |
| Species | N/A |
| Alias | LPScore |
| PositiveControl | LPSE.coli |
| NegativeControl | Normallymphnode(non-infected) |
| References | 1.DiPadova,FEetal;Identificationofwidelycross-reactiveandcross-protectiveanti-LPScoremonoclonalantibodies(Mabs).InfectImmun1993,61:3863 2.Bahrami,Setal;Monoclonalantibodytoendotoxinattenuateshemorrhage-inducedlunginjuryandmortalityinrats.CritCareMed1997,25:10303.Pollack,Metal;Dualeffectsoflipopolysaccharide(LPS)antibodiesoncellularuptakeofLPSandLPS-inducedproinflammatoryfunctions.JImmunol1997,151:3519 4.Müller-Loennies,Setal;Identificationofacross-reactiveepitopewidelypresentinlipopolysaccharidefromenterobacteriaandrecognizedbythecrossprotectivemonoclonalantibodyWN1222-5.JBiolChem2003,278:256185.Tsuneyoshi,Netal;ThefunctionalandstructuralpropertiesofMD-2requiredforlipopolysaccharidebindingareabsentinMD-1.JImmunol2005,174:340 6.Estes,Jetal;Damagedintestinalepithelialintegritylinkedtomicrobialtranslocationinpathogenicsimianimmunodeficiencyvirusinfections.PLOSpathogens2010,6:e10010527.Wang,Aetal;Anti-LPSTestStripfortheDetectionofFoodContaminatedwithSalmonellaandE.coli.MicrobialBiochemTechnol2011,3:2 8.Barclay,Getal;MonoclonalAntibodiestoEndotoxinCoreSegregateintoFamiliesofSpecificityandCross-ReactivitytoLipopolysaccharidesinELISAbyCorrelationClusterAnalysis.9.Cosgrove,Cetal;EarlyandnonreversIBLedecreaseofCD161++/MAITcellsinHIVinfection.Blood2013,121:951 10.Ruan,Xetal.InVitroO-AntigenLigaseAssay.MethodsMolBiol2013,1022:18511.Belcher,Jeta;HemetriggersTLR4signalingleADIngtoendothelialcellactivationandvaso-occlusioninmurinesicklecelldisease.Blood2014,123:377 |
| StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforatleastoneyear.Theexactexpirydateisindicatedonthelabel |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsibleforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
| Disease | Infectiousdiseases |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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