Hycult Biotech/CD36，人，单克隆抗体FA6-152，功能研究抗体，低内毒素/HM2122-FS/0.5 mg
商品编号：
HM2122-FS
品牌：
hycultbiotech
市场价：
¥20832.00
美元价：
12499.20
产品分类：
标签抗体
公司分类：
Tag_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:CD36,Human,mAbFA6-152,functionalstudyantibody,lowendotoxin
MonoclonalantibodyFA6-152recognizeshumanCD36(88-kDa),acellsurfaceclassBscavengerreceptor,alsoknownasthrombospondinreceptorCD36isaheavilyN-glycosylatedtransmembraneproteinof~88kDawithtwoshortintracellulardomainsandalargeextracellulardomain.Theproteinissensitiveforneuroaminidase,resultinginashiftfrom88to85kDa.CD36isexpressedonplatelets,maturemonocytesandmacrophages,microvascularendothelialcells,mammaryendothelialcells,duringstagesoferythroidcelldevelopmentandonsomemacrophagederiveddendriticcells.Theantibodyrecognizesadultandfetalmonocytes,plateletsandreticulocytes,butdoesn’tstainlymphocytesandgranulocytes.Reactivityhasalsobeenfoundinsmallintestine,kidney,liverandthyroid.CD36expressionisprimarilycontrolledbythetranscriptionheterodimerPPARg-RXR(peroxisomeproliferator-activatedreceptor-g-retinoid-X-receptor).CD36ispreferentiallyfoundwithinlipidrafts,whichfacilitatesitsassociationwithreceptors,signalingandadaptormolecules.Itisareceptorandtransporterofoxidizedlipidsandlongchainfattyacids.CD36hasbeenimplicatedinmanyBIOLOGicalprocessesincludingangiogenesis,phagocytosis,inflammation,andlipidandglucosemetabolism.Severalinvivomodelssupporttheroleofthethrombospondin/CD36systeminangiogenesisandtumorgrowth.AnimportantroleforCD36hasbeenfoundinMalariaasmajorreceptorforP.falciparum-infectedredbloodcells.CD36isassociatedwithSrc-familykinasesandwiththeintegrinsα3β1andα6β1.Recently,CD36hasbeenidentifiedasaproteinthatisrequiredfortolllikereceptor(TLR2)recognitionofdi-acylatedbacteriallipopeptidesandlipoteichoicacid4.FurThermore,CD36hasbeenshowntofunctionasphagocyticreceptorforapoptoticcells.ManydifferentligandshavebeenreportedtointeractwithCD36,suggestingthatCD36couldrecognizeastructure-baseddomainratherthanspecificcontactresidues.MonoclonalantibodyFA6-152blocksthebiologicalactivityofCD36byblockingcollagen/thrombospondinbinding.Theantibodyagglutinatesfetalbutnotadulterythrocytes.
Specifications:
| Catalognumber | HM2122-FS |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 0.5mg |
| Formulation | 0.5mgof0.2μmfilteredproteinGpurifiedantibodysolutioninPBSwithaconcentrationofatleast0.5mg/ml(exactconcentrationisindicatedonthelabel).Theendotoxinconcentrationis<24EU/mg,determinedwithHIT302LALAssay. |
| Application | Flowcytometry,Frozensections,Functionalstudies,Immunoassays,Immunofluorescence,Immunoprecipitation |
| ApplicationNotes | F:Tissueembeddedintissue-tek(forinstanceaortictissue)followedbyfreezinginliquidnitrogen;7-8 µmsections;air-dried;aceton-fixed;10%NGSasblock(Ref4). FC:AntibodyFA6-152stainstheextracellulardomainofCD36.Unfixedcells;2 µgper100.000cells.Positiveongranulocytes(Ref1). FS:Plateletaggregationandsecretionwasinducedby;1 µg/mlantibody(Ref2). IA:10 µg/mlantibodyascoatdilutedinTris-bufferedsaline;100 µm;l/well;o/natRT(Ref3). IF:unfixedcellswereincubatedfor30minutesat4 °CfollowedbyasecondaryFITCpolyclonalantibody;one-minutemethanolfixationbeforeanalysis(Ref1). IP:88kDasialoglycoproteininplatelets;85and88kDainHELcells.10 µgantibody/200 µgprotein |
| Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
| Immunogen | 20-Weeks-oldfetalerythrocytes |
| Isotype | MouseIgG1 |
| Species | Human |
| Alias | GPIV,Fattyacidtranslocase(FAT),plateletglycoprotein4,PASIV,Plateletcollagenreceptor,thrombospondinreceptor Genename:CD36 |
| PositiveControl | THP-1cells |
| References | 1.EdelmanPetal;AmonoclonalantibodyagainstanerythrocyteontogenicantigenidentifiesfetalandadulterythroidProgenitors.Blood1986,67:56. 2.KiefferNetal;Developmentregulatedexpressionofa78kDaerythroblastmembraneglycoproteinimmunologicallyrelatedtotheplateletthrombospondinreceptor.BiochemJ1989,262:8353.ThibertVetal;QuantitationofplateletglycoproteinIV(CD36)inhealthysubjectsandinpatientswithessentialthrombocythemiausinganimmunocaptureassay.ThrombHaemost1992,68:600 4.Nakata,Aetal;CD36,anovelreceptorforoxidizedlow-densitylipoproteins,ishighlyexpressedonlidip-ladenmacrophagesinhumanatheroscleroticaorta.ArteriosclerThrombVascBiol1999,19:13335.Ehara,Setal;Pathophysiologicalroleofoxidezedlow-densitylipoproteininplaqueinstABIlityincoronaryarterydiseases.JDiabComplic2002,16:60 6.Leonarduzzi,Getal;Oxidationasacrucialreactionforcholesteroltoinducetissuedegeneration:CD36overexpressioninhumanpromonocyticcellstreatedwithabiologicallyrelevantoxysterolmixture,AgingCell2008,7:3757.Leonarduzzi,Getal;Molecularsignalingoperatedbyadiet-compatIBLemixtureofoxysterolsinup-regulatingCD36receptorinCD68positivecells,Mol.Nutr.FoodRes.2010,54:S31 8.Finn,Aetal;HemoglobinDirectsMacrophageDifferentiationandPreventsFoamCellFormationinHumanAtheroscleroticPlaques,JAmCollCardiol.2012,59:1669.Gamba,P.etal.Interactionbetween24-hydroxycholesterol,oxidativestress,andamyloid-βinamplifyingneuronaldamageinAlzheimer’sdisease:threepartnersincrime.AgingCell2011,10:40310.Grange,P.A.etalProductionofSuperoxideAnionsbyKeratinocytesInitiatesP.acnes-InducedInflammationoftheSkin.PlosPathogens2009,5:100052711.Barth,Hetal.ScavengerReceptorClassBIsRequiredforHepatitisCVirusUptakeandCross-PresentationbyHumanDendriticCells.JofVirol.2008,82:346612.Nilsen,Netal.CellulartraffickingoflipoteichoicacidandToll-likereceptor2inrelationtosignaling;roleofCD14andCD36.JournalofLeukocyteBiology2008,84:280 |
| Storageandstability | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforatleastoneyear.Theexactexpirydateisindicatedonthelabel. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsibleforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
| Disease | Cardiologyandmetabolism,Infectiousdiseases |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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