Hycult Biotech/CD46，人，单克隆抗体M177，功能研究抗体，低内毒素/HM2103-FS/0.5 mg
商品编号：
HM2103-FS
品牌：
hycultbiotech
市场价：
¥26232.00
美元价：
15739.20
产品分类：
标签抗体
公司分类：
Tag_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:CD46,Human,mAbM177,functionalstudyantibody,lowendotoxin
ThemonoclonalantibodyM177recognizesCD46,alsodesignatedmembranecofactorprotein(MCP).CD46isa45-70kDaproteinwithgeneticandtissue-specificheterogeneity.-Itisexpressedoneverycellandtissue,withtheexceptionoferythrocytes.CD46servestoinhibitcomplementactivationonhosttissue.ItperformsthisfunctionbyservingasacofactorwhichbindstoC3bandC4b.ThisbindingispermittedbyfactorI,aserineproteaseofplasma,todegradeC3bandC4bandservestoprotectthehostcellagainstautologousattack.Italsoservesasareceptorformeaslesvirus.
FourisoformsofCD46predominateandarisebyalternativesplicingofasingleCD46gene.CD46CDNAencodesasignalsequencefollowedbyfourcomplementcontrolproteindomains(alsocalledshortconsensusrepeats(SCR)).ThemonoclonalantibodyM177reactswiththeSCR2domain.
FourisoformsofCD46predominateandarisebyalternativesplicingofasingleCD46gene.CD46CDNAencodesasignalsequencefollowedbyfourcomplementcontrolproteindomains(alsocalledshortconsensusrepeats(SCR)).ThemonoclonalantibodyM177reactswiththeSCR2domain.
Specifications:
| Catalognumber | HM2103-FS |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 0.5mg |
| Formulation | 0.5mgof0.2μmproteinGpurifiedantibodysolutioninPBS(exactconcentrationisindicatedonthelabel). |
| Application | Flowcytometry,Frozensections,Functionalstudies,Immunoassays,Immunoprecipitation,Westernblot |
| ApplicationNotes | FC:AntibodyM177stainstheextracellulardomainofCD46. IP:107cellswerelysedandimmunoprecipitatedwith25 µgM177antibodyand25 µgproteinG-sepharose W:Anon-reducedsampletreatmentandSDS-pagewasused.Thebandsizeis45-70kDa(Ref1and2). |
| Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.Forfunctionalstudies,invitrodilutionshavetobeoptimizedinuser’sexperimentalsetting. |
| Immunogen | PurifiedMCP |
| Isotype | MouseIgG1 |
| Species | Human |
| Alias | Membranecofactorprotein,TLX,trophoblastleucocytecommonantigen Genename:CD46 |
| PositiveControl | CD46isexpressedoneverycellandtissue |
| NegativeControl | Erythrocytes |
| References | 1.Seya,Tetal;Quantitativeanalysisofmembranecofactorprotein(MCP)ofcomplement.HighexpressionofMCPonhumanleukemiacelllines,whichisdown-regulatedduringcelldifferentiation.JImmunol1990,145:238 2.Iwata,Ketal;DiversityofsitesformeaslesvirusbindingandforinactivationofcomplementC3bandC4bonmembranecofactorproteinCD46.JBiolChem1995,270:15148 3.Kurita-Taniguchi,Metal;FunctionalmodulationofhumanmacrophagesthroughCD46(Measlesvirusreceptor):productionofIL-12p40andnitricoxideinassociationwithrecruitmentofprotein-tyrosinephosphataseSHP-1toCD46.JImmunol2000,165:5143 4.Kurita-Taniguchi,Metal;MolecularassemblyofCD46withCD9,alpha3-beta1integrinandproteintyrosinephosphataseSHP-1inhumanmacrophagesthroughdifferentiationbyGM-CSF.MolImmunol2001,38:689 5.Loré,Ketal;MyeloidandplasmacytoiddentriticcellsaresusceptIBLetorecombinantadenovirusvectorsandstimulatepolyfunctionalmemoryTcellresponses.JImmunol2007,179:1721 6.Adams,Wetal;AttenuationofCD4+T-cellfunctionbyhumanadenovirustype35ismediatedbytheknobprotein.JGenVirol2012,93:1339 7.Johnson,Metal;TypeIIFNInducedbyAdenovirusSEROtypes28and35HasMultipleEffectsonTCellImmunogenicity.JImmunol2012,188:6109 8.Teigler,Jetal;VaccinationwithAdenovirusSerotypes35,26,and48ElicitsHigherLevelsofInnateCytokineResponsesthanAdenovirusSerotype5inRhesusMonkeys.JVirol2012,86:9590 9.Abbink,Petal;ConstructionandEvaluationofNovelRhesusMonkeyAdenovirusVaccineVectors.JVirol2015,89:1512 10.Fujiyuki,Tetal;Ameaslesvirusselectivelyblindtosignalinglymphocyticactivationmoleculeshowsanti-tumoractivityagainstlungcancercells.Oncotarget2015,6:24895 |
| StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsibleforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
| Disease | Infectiousdiseases,Tumorimmunology |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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