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Hycult Biotech/LL-37/CAP-18，人，单克隆抗体3D11，功能研究抗体，低内毒素/HM2070-FS/0.5 mg
Hycult Biotech/LL-37/CAP-18，人，单克隆抗体3D11，功能研究抗体，低内毒素/HM2070-FS/0.5 mg
商品编号：
HM2070-FS
品牌：
hycultbiotech
市场价：
¥26232.00
美元价：
15739.20
产品分类：
标签抗体
公司分类：
Tag_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:LL-37/CAP-18,Human,mAb3D11,functionalstudyantibody,lowendotoxin
Cationicantimicrobialpeptides,suchascathelicidinsplayimportantrolesinthedefenseagainstinfectionsbyeliminatingawiderangeofpathogens.hCAP-18/LL-37istheonlycathelicidinidentifiedinhumansanditisproducedbyneutrophils,monocytes,mastcells,andepithelialcells.Itisstoredinthesecellsasapropeptide,whichcanbecleavedextracellularlybyenzymeslikeproteinase3,resultingintheformationofLL-37andacathelinpart.LL-37isanamphipathica-helicalpeptidethatcanaffectbothplanktonicbacteriaandthoseresidinginbiofilms,virusessuchasHIVandfungi,anditcanneutralizeLPSandlipoteichoicacid(LTA).Inadditiontoitsantimicrobialactions,LL-37participatesattheinterfaceofinnateandadaptiveimmunitybymodulatingcytokineandchemokineproductionbyarangeofcelltypes,chemoattractingvariousimmuneeffectorcellsandmesenchymalstemcells,regulatingautophagyinconjunctionwithvitaminD,andstimulatingangiogenesisandwoundhealing.LL-37doesnotonlykillbacteria,butcanalsomodulate(suppress)neutrophilapoptosisviatheactivationofFPRL1andP2X7inbacterialinfections.Suppressionofneutrophilapoptosisresultsintheprolongationoftheirlifespan,andmaybeadvantageousforhostdefenseagainstbacterialinvasion.Moreover,itfunctionsasachemotacticagentforneutrophils,monocytesandTcells.LL-37ismarkedlyresistanttoproteolyticdegradationandtoalimitedextentalsocytotoxictowardsmammaliancells.TheantibodydetectsbothfreeLL37(MW4kDa)andcompletehCAP18(MW19kDa).Themonoclonalantibody3D11canbeusedforneutralizingthefunctionofLL-37.TheABIlityofmastcellstokillpneumococciwassignificantattenuatedusingthemonoclonalantibody3D11.
Specifications:
| Catalognumber | HM2070-FS |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 0.5mg |
| Formulation | 0.5mg(100µg/ml)0.2µmfilteredantibodysolutioninPBS(exactconcentrationisindicatedonthelabel) |
| Application | Functionalstudies,Immunofluorescence,Paraffinsections,Westernblot |
| ApplicationNotes | FS:Additionoftheantibodyreduces,after3hincubation,thecytotoxicityofD39pneumococciinducedbyHumanlungmastcells(HLMCs). IF:AntibodywasusedonisolatedneutrophilsfixedwithPMAonlysinatedglassslidesandblockedwithPBS-5%goatserum-1%BSA. P:Paraffinsectionsweredeparaffinized,rehydratedandendogenousperoxidasewasquenchedusing0.3%H2O2inmethanolfor20min.Afterantigenretrievalsectionswereincubatedwith1.5ug/mlantibody. |
| Use | Forimmunohistology,dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
| Immunogen | HumanLL-37peptide |
| Isotype | MouseIgG1 |
| Species | Human |
| Alias | Cathelicidinantimicrobialpeptide,hCAP-18,AntibacterialproteinLL-37,Cap-18,CRAMP;HSD26;FALL39;FALL-39;CAMP |
| PositiveControl | Neutrophils |
| References | 1.Sørensen,Oetal;Humancathelicidin,hCAP-18,isprocessedtotheantimicrobialpeptideLL-37byextracellularcleavagewithproteinase3.Blood2001,97:3951 2.Nell,Metal;BacterialproductsincreaseexpressionofthehumancathelicidinhCAP-18/LL-37inculturedhumansinusepithelialcells.FEMSImmunolMedMicrobiol2004,42:225 3.Kessenbrock,Ketal;Nettingneutrophilsinautoimmunesmall-vesselvasculitis.Naturemed2009,15:623 4.Cruse,Getal;Humanlungmastcellsmediatepneumococcalcelldeathinresponsetoactivationbypneumolysin.JImmunol2010,184:7108 5.Coffelt,Setal;Thepro-inflammatorypeptideLL-31promotesovariantumorprogressionthroughrecruitmentofmultipotentmesenchymalstromalcells.PNAS2009,106(10):p3806-3811 |
| Storageandstability | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
| Disease | Autoimmunity,Infectiousdiseases |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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